PCID2 (NM_018386) Human Tagged ORF Clone

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SKU
RC221802
PCID2 (Myc-DDK-tagged)-Human PCI domain containing 2 (PCID2), transcript variant 3
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PCID2
Synonyms F10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221802 representing NM_018386
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCACATTACCATTAACCAGTACCTGCAGCAGGTGTACGAAGCCATCGACAGCAGAGATGGAGCAT
CTTGTGCAGAGTTGGTGTCTTTTAAACATCCTCATGTTGCAAACCCACGACTTCAAATGGCCTCTCCAGA
GGAGAAGTGTCAACAAGTCTTGGAACCCCCTTATGATGAAATGTTTGCAGCTCATTTAAGGTGCACTTAT
GCAGTGGGGAATCATGACTTCATAGAGGCATACAAGTGCCAGACCGTGATAGTCCAATCATTCTTGCGAG
CATTCCAGGCCCACAAAGAAGAAAACTGGGCTCTGCCTGTCATGTATGCAGTAGCGCTTGACCTTCGAGT
GTTTGCCAATAATGCAGATCAACAGTTGGTAAAGAAAGGAAAAAGCAAAGTTGGGGACATGTTGGAAAAA
GCAGCAGAGTTACTGATGAGCTGTTTCCGGGTCTGTGCCAGCGACACCCGTGCTGGTATAGAGGACTCTA
AGAAGTGGGGCATGCTGTTTCTGGTGAACCAGCTGTTTAAAATCTACTTCAAGATCAACAAACTCCATTT
ATGTAAACCCCTAATTAGAGCAATTGACAGCTCAAACCTGAAAGACGATTACAGCACTGCACAGAGAGTA
ACATACAAATACTACGTTGGACGCAAGGCTATGTTTGACAGCGATTTTAAGCAAGCTGAGGAGTACCTGT
CATTTGCCTTTGAGCATTGTCACCGTTCTAGTCAGAAGAACAAAAGGATGATTCTGATCTATTTGCTTCC
AGTAAAAATGCTATTGGGTCACATGCCCACTGTGGAGCTCCTGAAAAAGTATCACCTGATGCAGTTTGCG
GAAGTAACCAGAGCTGTGAGCGAGGGCAACCTGCTGCTGCTGCACGAGGCGCTGGCGAAGCACGAGGCCT
TCTTCATTCGCTGCGGAATCTTCCTCATCCTGGAGAAGCTGAAGATCATCACCTACAGGAACCTCTTTAA
GAAAGTGTATTTGTTACTGAAAACACACCAGCTGTCTCTGGATGCTTTTCTGGTTGCCTTGAAGTTCATG
CAGGTGGAGGACGTGGACATTGACGAAGTTCAGTGTATTCTGGCTAACTTGATATACATGGGACACGTCA
AAGGCTACATATCGCATCAGCATCAGAAGCTGGTGGTCAGCAAGCAGAACCCATTTCCTCCCCTGTCCAC
GGTGTGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC221802 representing NM_018386
Red=Cloning site Green=Tags(s)

MAHITINQYLQQVYEAIDSRDGASCAELVSFKHPHVANPRLQMASPEEKCQQVLEPPYDEMFAAHLRCTY
AVGNHDFIEAYKCQTVIVQSFLRAFQAHKEENWALPVMYAVALDLRVFANNADQQLVKKGKSKVGDMLEK
AAELLMSCFRVCASDTRAGIEDSKKWGMLFLVNQLFKIYFKINKLHLCKPLIRAIDSSNLKDDYSTAQRV
TYKYYVGRKAMFDSDFKQAEEYLSFAFEHCHRSSQKNKRMILIYLLPVKMLLGHMPTVELLKKYHLMQFA
EVTRAVSEGNLLLLHEALAKHEAFFIRCGIFLILEKLKIITYRNLFKKVYLLLKTHQLSLDAFLVALKFM
QVEDVDIDEVQCILANLIYMGHVKGYISHQHQKLVVSKQNPFPPLSTVC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018386
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018386.5
RefSeq Size 1786 bp
RefSeq ORF 1200 bp
Locus ID 55795
UniProt ID Q5JVF3
Cytogenetics 13q34
MW 45.8 kDa
Summary This gene encodes a component of the TREX-2 complex (transcription and export complex 2), which regulates mRNA export from the nucleus. This protein regulates expression of Mad2 mitotic arrest deficient-like 1, a cell division checkpoint protein. This protein also interacts with and stabilizes Brca2 (breast cancer 2) protein. Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2016
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Documents
Resources
"NM_018386" in other vectors (4)
SKU Description Size Price
RC221802L3 Lenti ORF clone of Human PCI domain containing 2 (PCID2), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC221802L4 Lenti ORF clone of Human PCI domain containing 2 (PCID2), transcript variant 3, mGFP tagged 10 ug
$757.00
RG221802 PCID2 (tGFP-tagged) - Human PCI domain containing 2 (PCID2), transcript variant 3 10 ug
$657.00
SC313541 PCID2 (untagged)-Human PCI domain containing 2 (PCID2), transcript variant 3 10 ug
$503.00

Plasmid Map for RC221802

Download sequence for

Cloning scheme for RC221802

Circular map for RC221802

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