DCDC1 (NM_181807) Human Tagged ORF Clone

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SKU
RC221749
DCDC1 (Myc-DDK-tagged)-Human doublecortin domain containing 1 (DCDC1)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DCDC1
Synonyms DCDC5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221749 representing NM_181807
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAAAACAGGAGCAGAAGATCACAGAGAAGCACTATCTCAGTCTTCCTTATCCCTCTTGACTGAAG
CAATGGAAGTATTACAGCAAAGTAGCCCTGAAGGCACTTTGGATGGGAATACTGTAAACCCAATTTACAA
ATATATTTTGAATGATTTACCAAGAGAGTTTATGTCATCCCAGGCAAAAGCAGTTATTAAAACTACTGAT
GATTATTTGCAGTCTCAGTTTGGCCCCAACAGACTCGTGCATTCAGCAGCAGTATCAGAAGGGTCAGGAC
TTCAAGATTGCTCCACACATCAAACAGCATCAGATCACAGCCATGATGAAATATCAGACCTAGATAGCTA
CAAATCAAACAGTAAAAACAATTCTTGTTCTATATCAGCATCCAAGAGAAACAGACCTGTCAGTGCTCCA
GTGGGTCAACTGAGGGTTGCAGAGTTCTCTTCTTTAAAATTTCAGTCAGCCCGGAATTGGCAGAAATTGT
CTCAAAGACACAAACTTCAACCAAGAGTGATTAAAGTAACAGCTTACAAAAATGGATCTAGAACAGTCTT
TGCCAGAGTTACTGTACCAACCATCACCTTGCTGCTGGAGGAGTGCACAGAAAAGCTGAATCTGAACATG
GCCGCAAGACGAGTGTTCTTGGCAGACGGCAAGGAAGCCCTCGAACCTGAAGATATACCCCATGAAGCCG
ATGTTTATGTTTCAACGGGAGAGCCCTTTTTAAATCCATTCAAAAAAATTAAAGACCATCTGTTGTTAAT
TAAGAAAGTAACTTGGACAATGAATGGGTTGATGCTTCCTACTGATATCAAAAGACGGAAAACCAAGCCT
GTTCTTTCTATTAGAATGAAGAAACTTACTGAGAGGACCTCAGTCCGAATTCTGTTCTTTAAGAATGGCA
TGGGGCAGGATGGGCATGAGATTACAGTGGGAAAAGAAACAATGAAAAAGGTTTTAGATACTTGTACAAT
AAGAATGAATCTAAATTTACCAGCCAGATATTTTTATGATTTGTATGGCAGAAAAATTGAAGATATTTCA
AAAGGAAAGCAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC221749 representing NM_181807
Red=Cloning site Green=Tags(s)

MAKTGAEDHREALSQSSLSLLTEAMEVLQQSSPEGTLDGNTVNPIYKYILNDLPREFMSSQAKAVIKTTD
DYLQSQFGPNRLVHSAAVSEGSGLQDCSTHQTASDHSHDEISDLDSYKSNSKNNSCSISASKRNRPVSAP
VGQLRVAEFSSLKFQSARNWQKLSQRHKLQPRVIKVTAYKNGSRTVFARVTVPTITLLLEECTEKLNLNM
AARRVFLADGKEALEPEDIPHEADVYVSTGEPFLNPFKKIKDHLLLIKKVTWTMNGLMLPTDIKRRKTKP
VLSIRMKKLTERTSVRILFFKNGMGQDGHEITVGKETMKKVLDTCTIRMNLNLPARYFYDLYGRKIEDIS
KGKH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181807
ORF Size 1062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181807.4
RefSeq Size 1719 bp
RefSeq ORF 1065 bp
Locus ID 341019
UniProt ID Q6ZRR9, M0R2J8
Cytogenetics 11p13
MW 39.7 kDa
Summary This gene encodes a member of the doublecortin family. The protein encoded by this gene is a hydrophilic, intracellular protein. It contains a single doublecortin domain and is unable to bind microtubules and to regulate microtubule polymerization. This gene is mainly expressed in adult testis. It does not have a mouse homolog. provided by RefSeq, Sep 2010
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Documents
Resources
"NM_181807" in other vectors (4)
SKU Description Size Price
RC221749L3 Lenti ORF clone of Human doublecortin domain containing 1 (DCDC1), Myc-DDK-tagged 10 ug
$757.00
RC221749L4 Lenti ORF clone of Human doublecortin domain containing 1 (DCDC1), mGFP tagged 10 ug
$757.00
RG221749 DCDC1 (tGFP-tagged) - Human doublecortin domain containing 1 (DCDC1) 10 ug
$657.00
SC307377 DCDC1 (untagged)-Human doublecortin domain containing 1 (DCDC1) 10 ug
$457.00

Plasmid Map for RC221749

Download sequence for

Cloning scheme for RC221749

Circular map for RC221749

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