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REV1 (NM_016316) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC221742L1
Lenti ORF clone of Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1, Myc-DDK-tagged
ORF Plasmid:
DDK DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,389.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol REV1
Synonyms AIBP80; REV1L
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC221742).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016316
ORF Size 3753 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016316.1
RefSeq Size 4276 bp
RefSeq ORF 3756 bp
Locus ID 51455
UniProt ID Q9UBZ9
Cytogenetics 2q11.2
Domains BRCT, IMS
Protein Families Druggable Genome
MW 138.1 kDa
Summary This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. provided by RefSeq, Mar 2016
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"NM_016316" in other vectors (6)
SKU Description Size Price
RC221742 REV1 (Myc-DDK-tagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1 10 ug
$1,089.00
RC221742L2 Lenti ORF clone of Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1, mGFP tagged 10 ug
$1,389.00
RC221742L3 Lenti ORF clone of Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,389.00
RC221742L4 Lenti ORF clone of Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1, mGFP tagged 10 ug
$1,389.00
RG221742 REV1 (tGFP-tagged) - Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1 10 ug
$789.00 $1,289.00
SC114312 REV1 (untagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 1 10 ug
$1,090.00

Plasmid Map for RC221742L1

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Cloning scheme for RC221742L1
Circular map for RC221742L1

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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