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Semaphorin 5A (SEMA5A) (NM_003966) Human Tagged ORF Clone

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SKU
RC221685
SEMA5A (Myc-DDK-tagged)-Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $935.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Semaphorin 5A
Synonyms SEMAF; semF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221685 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGAACCTGTGTTATAGCATGGCTGTTCTCAAGCCTGGGGCTGTGGAGACTCGCCCACCCAGAGG
CCCAGGGTACGACTCAGTGCCAGAGAACCGAGCATCCAGTCATCTCCTATAAAGAAATTGGCCCCTGGTT
ACGGGAGTTCAGAGCGAAGAATGCTGTGGATTTCTCGCAGTTAACATTTGACCCAGGACAGAAAGAACTT
GTTGTAGGAGCAAGAAACTACCTCTTCAGGTTACAGCTTGAGGATCTGTCTCTTATCCAGGCTGTGGAAT
GGGAGTGTGATGAAGCTACCAAAAAGGCCTGTTACAGCAAAGGCAAATCAAAGGAGGAATGTCAGAACTA
CATCCGGGTGCTTCTGGTGGGTGGCGACCGGTTATTCACCTGTGGGACCAATGCATTCACGCCTGTCTGC
ACCAACCGCTCGTTGAGCAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGTCCCTACA
GTCCCCAGCACAATTCCACAGCGCTCCTCACAGCTGGTGGGGAGCTCTATGCTGCTACAGCCATGGATTT
TCCAGGACGTGATCCTGCCATTTACCGAAGCCTAGGCATTTTACCTCCTCTCCGCACGGCGCAGTACAAC
TCCAAATGGCTCAATGAGCCAAACTTTGTGTCATCTTATGACATCGGAAATTTTACCTACTTCTTTTTCC
GAGAAAATGCAGTAGAGCATGACTGTGGGAAAACAGTGTTCTCCAGAGCTGCCCGGGTGTGCAAGAACGA
TATTGGTGGGCGCTTCCTGCTGGAAGACACCTGGACCACATTCATGAAGGCTCGCCTGAACTGCTCCCGT
CCTGGGGAAGTCCCCTTTTACTACAACGAATTGCAGAGTACTTTCTTCCTGCCTGAGCTGGATTTGATCT
ATGGCATCTTTACCACCAATGTGAACAGCATTGCGGCCTCAGCTGTGTGCGTCTTCAACCTGAGCGCCAT
CGCGCAGGCCTTCTCTGGGCCCTTCAAGTACCAAGAAAACTCGCGCTCGGCCTGGCTACCGTATCCCAAC
CCAAACCCCCACTTCCAGTGTGGCACCGTGGACCAGGGCCTGTACGTGAACCTGACCGAGAGAAATCTGC
AGGATGCTCAGAAGTTCATTCTGATGCATGAGGTGGTACAGCCAGTGACCACAGTGCCCTCCTTCATGGA
GGACAATAGCCGCTTTTCCCACGTGGCAGTCGACGTGGTGCAGGGCAGAGAAGCGCTCGTCCACATCATC
TATTTGGCCACAGATTACGGAACCATTAAGAAAGTGCGGGTACCCCTGAATCAGACCTCAAGCAGCTGTT
TGCTGGAAGAGATTGAGCTCTTCCCTGAGAGGCGGAGGGAGCCCATCAGGAGCCTGCAGATCCTGCACAG
CCAGAGTGTCCTGTTCGTGGGCCTGCGGGAGCACGTGGTCAAGATCCCCCTGAAGAGGTGCCAGTTCTAC
CGCACACGCAGCACCTGCATTGGGGCCCAGGACCCTTACTGTGGCTGGGATGTGGTAATGAAGAAATGCA
CAAGCCTGGAGGAGAGCCTGAGCATGACGCAGTGGGAACAGAGCATCTCTGCGTGTCCGACCAGGAATCT
CACCGTGGATGGGCACTTTGGTGTGTGGTCTCCGTGGACGCCTTGCACGCACACAGATGGCAGCGCCGTG
GGATCCTGCCTCTGTCGAACCCGCTCCTGCGACAGCCCGGCCCCGCAGTGTGGTGGCTGGCAGTGCGAGG
GCCCTGGCATGGAGATCGCCAACTGTTCCAGGAACGGAGGCTGGACTCCCTGGACCTCGTGGTCTCCCTG
CAGCACTACCTGTGGGATCGGCTTCCAGGTGCGGCAGCGCTCCTGCAGCAACCCCACTCCCAGGCACGGG
GGCCGGGTGTGCGTGGGACAGAACCGCGAGGAAAGATACTGCAATGAACATTTGCTATGTCCCCCACACA
TGTTCTGGACAGGCTGGGGTCCTTGGGAACGGTGCACAGCCCAATGCGGGGGTGGCATTCAAGCTCGCCG
CAGGATCTGTGAGAATGGGCCTGACTGTGCAGGCTGCAATGTGGAGTACCAGTCTTGCAACACCAACCCG
TGTCCTGAGCTGAAGAAGACCACGCCCTGGACACCCTGGACACCTGTCAACATCTCTGACAACGGCGGCC
ACTATGAGCAACGATTCCGATACACATGCAAAGCCCGCCTGGCTGATCCGAATTTGCTGGAAGTGGGAAG
ACAGAGAATCGAAATGCGGTACTGTTCTAGCGACGGCACCAGTGGCTGCTCCACAGATGGGCTTTCTGGG
GATTTCCTGCGTGCTGGGAGATACTCTGCCCACACGGTCAACGGGGCTTGGTCAGCCTGGACGTCGTGGT
CACAGTGCAGCCGTGACTGCAGCAGGGGCATTCGGAACCGGAAGCGTGTTTGCAACAACCCCGAACCCAA
GTATGGGGGAATGCCTTGCCTTGGCCCATCTCTGGAATACCAGGAATGCAACATTTTGCCCTGCCCAGTG
GATGGCGTGTGGTCTTGCTGGTCCCCCTGGACAAAATGTTCAGCAACATGCGGCGGTGGACACTATATGA
GGACCCGCTCTTGCTCCAATCCAGCCCCGGCCTATGGAGGGGACATCTGCCTGGGGCTGCACACAGAAGA
GGCACTCTGCAACACGCAGCCCTGCCCAGAGAGCTGGTCGGAGTGGTCGGACTGGTCTGAGTGTGAAGCC
TCTGGCGTCCAAGTCCGCGCCCGCCAGTGCATCCTCCTGTTCCCCATGGGCAGCCAGTGCTCCGGGAACA
CCACGGAGAGCCGGCCGTGTGTGTTTGACTCTAATTTCATCCCAGAAGTATCTGTGGCAAGATCCAGTAG
CGTAGAAGAGAAAAGGTGTGGAGAGTTCAACATGTTCCACATGATCGCCGTGGGGCTGAGCAGCTCCATC
CTCGGCTGCCTCCTCACCCTGCTCGTCTATACTTACTGCCAGCGGTACCAGCAGCAATCCCACGATGCGA
CTGTCATCCACCCCGTCTCACCTGCCCCCCTTAATACCAGCATAACCAACCACATCAACAAACTGGACAA
GTACGACTCGGTGGAGGCCATCAAGGCATTTAACAAAAACAACTTGATCCTAGAGGAAAGAAACAAATAC
TTCAACCCACATCTCACTGGGAAGACCTATTCTAATGCCTACTTTACAGATCTCAATAATTATGATGAGT
AC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221685 protein sequence
Red=Cloning site Green=Tags(s)

MKGTCVIAWLFSSLGLWRLAHPEAQGTTQCQRTEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKEL
VVGARNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVC
TNRSLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYN
SKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSR
PGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAIAQAFSGPFKYQENSRSAWLPYPN
PNPHFQCGTVDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGREALVHII
YLATDYGTIKKVRVPLNQTSSSCLLEEIELFPERRREPIRSLQILHSQSVLFVGLREHVVKIPLKRCQFY
RTRSTCIGAQDPYCGWDVVMKKCTSLEESLSMTQWEQSISACPTRNLTVDGHFGVWSPWTPCTHTDGSAV
GSCLCRTRSCDSPAPQCGGWQCEGPGMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHG
GRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQARRRICENGPDCAGCNVEYQSCNTNP
CPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLADPNLLEVGRQRIEMRYCSSDGTSGCSTDGLSG
DFLRAGRYSAHTVNGAWSAWTSWSQCSRDCSRGIRNRKRVCNNPEPKYGGMPCLGPSLEYQECNILPCPV
DGVWSCWSPWTKCSATCGGGHYMRTRSCSNPAPAYGGDICLGLHTEEALCNTQPCPESWSEWSDWSECEA
SGVQVRARQCILLFPMGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMIAVGLSSSI
LGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSITNHINKLDKYDSVEAIKAFNKNNLILEERNKY
FNPHLTGKTYSNAYFTDLNNYDEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003966
ORF Size 3222 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003966.3
RefSeq Size 11825 bp
RefSeq ORF 3225 bp
Locus ID 9037
UniProt ID Q13591, X5DR95
Cytogenetics 5p15.31
Protein Families Transmembrane
Protein Pathways Axon guidance
MW 120.6 kDa
Summary This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.provided by RefSeq, Jan 2010
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Documents
Resources
"NM_003966" in other vectors (4)
SKU Description Size Price
RC221685L3 Lenti ORF clone of Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), Myc-DDK-tagged 10 ug
$1,235.00
RC221685L4 Lenti ORF clone of Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), mGFP tagged 10 ug
$1,235.00
RG221685 SEMA5A (tGFP-tagged) - Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A) 10 ug
$1,135.00
SC303407 SEMA5A (untagged)-Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A) 10 ug
$936.00

Plasmid Map for RC221685

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221685
Circular map for RC221685

file-alt Citations

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