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Semaphorin 5A (SEMA5A) (NM_003966) Human Untagged Clone

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SKU
SC303407
SEMA5A (untagged)-Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A)
  $936.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Semaphorin 5A
Synonyms SEMAF; semF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC303407 representing NM_003966.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAGGGAACCTGTGTTATAGCATGGCTGTTCTCAAGCCTGGGGCTGTGGAGACTCGCCCACCCAGAG
GCCCAGGGTACGACTCAGTGCCAGAGAACCGAGCATCCAGTCATCTCCTATAAAGAAATTGGCCCCTGG
TTACGGGAGTTCAGAGCGAAGAATGCTGTGGATTTCTCGCAGTTAACATTTGACCCAGGACAGAAAGAA
CTTGTTGTAGGAGCAAGAAACTACCTCTTCAGGTTACAGCTTGAGGATCTGTCTCTTATCCAGGCTGTG
GAATGGGAGTGTGATGAAGCTACCAAAAAGGCCTGTTACAGCAAAGGCAAATCAAAGGAGGAATGTCAG
AACTACATCCGGGTGCTTCTGGTGGGTGGCGACCGGTTATTCACCTGTGGGACCAATGCATTCACGCCT
GTCTGCACCAACCGCTCGTTGAGCAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGT
CCCTACAGTCCCCAGCACAATTCCACAGCGCTCCTCACAGCTGGTGGGGAGCTCTATGCTGCTACAGCC
ATGGATTTTCCAGGACGTGATCCTGCCATTTACCGAAGCCTAGGCATTTTACCTCCTCTCCGCACGGCG
CAGTACAACTCCAAATGGCTCAATGAGCCAAACTTTGTGTCATCTTATGACATCGGAAATTTTACCTAC
TTCTTTTTCCGAGAAAATGCAGTAGAGCATGACTGTGGGAAAACAGTGTTCTCCAGAGCTGCCCGGGTG
TGCAAGAACGATATTGGTGGGCGCTTCCTGCTGGAAGACACCTGGACCACATTCATGAAGGCTCGCCTG
AACTGCTCCCGTCCTGGGGAAGTCCCCTTTTACTACAACGAATTGCAGAGTACTTTCTTCCTGCCTGAG
CTGGATTTGATCTATGGCATCTTTACCACCAATGTGAACAGCATTGCGGCCTCAGCTGTGTGCGTCTTC
AACCTGAGCGCCATCGCGCAGGCCTTCTCTGGGCCCTTCAAGTACCAAGAAAACTCGCGCTCGGCCTGG
CTACCGTATCCCAACCCAAACCCCCACTTCCAGTGTGGCACCGTGGACCAGGGCCTGTACGTGAACCTG
ACCGAGAGAAATCTGCAGGATGCTCAGAAGTTCATTCTGATGCATGAGGTGGTACAGCCAGTGACCACA
GTGCCCTCCTTCATGGAGGACAATAGCCGCTTTTCCCACGTGGCAGTCGACGTGGTGCAGGGCAGAGAA
GCGCTCGTCCACATCATCTATTTGGCCACAGATTACGGAACCATTAAGAAAGTGCGGGTACCCCTGAAT
CAGACCTCAAGCAGCTGTTTGCTGGAAGAGATTGAGCTCTTCCCTGAGAGGCGGAGGGAGCCCATCAGG
AGCCTGCAGATCCTGCACAGCCAGAGTGTCCTGTTCGTGGGCCTGCGGGAGCACGTGGTCAAGATCCCC
CTGAAGAGGTGCCAGTTCTACCGCACACGCAGCACCTGCATTGGGGCCCAGGACCCTTACTGTGGCTGG
GATGTGGTAATGAAGAAATGCACAAGCCTGGAGGAGAGCCTGAGCATGACGCAGTGGGAACAGAGCATC
TCTGCGTGTCCGACCAGGAATCTCACCGTGGATGGGCACTTTGGTGTGTGGTCTCCGTGGACGCCTTGC
ACGCACACAGATGGCAGCGCCGTGGGATCCTGCCTCTGTCGAACCCGCTCCTGCGACAGCCCGGCCCCG
CAGTGTGGTGGCTGGCAGTGCGAGGGCCCTGGCATGGAGATCGCCAACTGTTCCAGGAACGGAGGCTGG
ACTCCCTGGACCTCGTGGTCTCCCTGCAGCACTACCTGTGGGATCGGCTTCCAGGTGCGGCAGCGCTCC
TGCAGCAACCCCACTCCCAGGCACGGGGGCCGGGTGTGCGTGGGACAGAACCGCGAGGAAAGATACTGC
AATGAACATTTGCTATGTCCCCCACACATGTTCTGGACAGGCTGGGGTCCTTGGGAACGGTGCACAGCC
CAATGCGGGGGTGGCATTCAAGCTCGCCGCAGGATCTGTGAGAATGGGCCTGACTGTGCAGGCTGCAAT
GTGGAGTACCAGTCTTGCAACACCAACCCGTGTCCTGAGCTGAAGAAGACCACGCCCTGGACACCCTGG
ACACCTGTCAACATCTCTGACAACGGCGGCCACTATGAGCAACGATTCCGATACACATGCAAAGCCCGC
CTGGCTGATCCGAATTTGCTGGAAGTGGGAAGACAGAGAATCGAAATGCGGTACTGTTCTAGCGACGGC
ACCAGTGGCTGCTCCACAGATGGGCTTTCTGGGGATTTCCTGCGTGCTGGGAGATACTCTGCCCACACG
GTCAACGGGGCTTGGTCAGCCTGGACGTCGTGGTCACAGTGCAGCCGTGACTGCAGCAGGGGCATTCGG
AACCGGAAGCGTGTTTGCAACAACCCCGAACCCAAGTATGGGGGAATGCCTTGCCTTGGCCCATCTCTG
GAATACCAGGAATGCAACATTTTGCCCTGCCCAGTGGATGGCGTGTGGTCTTGCTGGTCCCCCTGGACA
AAATGTTCAGCAACATGCGGCGGTGGACACTATATGAGGACCCGCTCTTGCTCCAATCCAGCCCCGGCC
TATGGAGGGGACATCTGCCTGGGGCTGCACACAGAAGAGGCACTCTGCAACACGCAGCCCTGCCCAGAG
AGCTGGTCGGAGTGGTCGGACTGGTCTGAGTGTGAAGCCTCTGGCGTCCAAGTCCGCGCCCGCCAGTGC
ATCCTCCTGTTCCCCATGGGCAGCCAGTGCTCCGGGAACACCACGGAGAGCCGGCCGTGTGTGTTTGAC
TCTAATTTCATCCCAGAAGTATCTGTGGCAAGATCCAGTAGCGTAGAAGAGAAAAGGTGTGGAGAGTTC
AACATGTTCCACATGATCGCCGTGGGGCTGAGCAGCTCCATCCTCGGCTGCCTCCTCACCCTGCTCGTC
TATACTTACTGCCAGCGGTACCAGCAGCAATCCCACGATGCGACTGTCATCCACCCCGTCTCACCTGCC
CCCCTTAATACCAGCATAACCAACCACATCAACAAACTGGACAAGTACGACTCGGTGGAGGCCATCAAG
GCATTTAACAAAAACAACTTGATCCTAGAGGAAAGAAACAAATACTTCAACCCACATCTCACTGGGAAG
ACCTATTCTAATGCCTACTTTACAGATCTCAATAATTATGATGAGTACTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_003966
Insert Size 3225 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003966.1
RefSeq Size 11825 bp
RefSeq ORF 3225 bp
Locus ID 9037
UniProt ID Q13591
Cytogenetics 5p15.31
Protein Families Transmembrane
Protein Pathways Axon guidance
MW 120.6 kDa
Summary This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.provided by RefSeq, Jan 2010
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"NM_003966" in other vectors (4)
SKU Description Size Price
RC221685 SEMA5A (Myc-DDK-tagged)-Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A) 10 ug
$935.00
RC221685L3 Lenti ORF clone of Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), Myc-DDK-tagged 10 ug
$1,235.00
RC221685L4 Lenti ORF clone of Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A), mGFP tagged 10 ug
$1,235.00
RG221685 SEMA5A (tGFP-tagged) - Human sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A) 10 ug
$1,135.00

file-alt Citations

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