AMCase (CHIA) (NM_201653) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC221549L1
Lenti ORF clone of Human chitinase, acidic (CHIA), transcript variant 4, Myc-DDK-tagged
  $986.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AMCase
Synonyms AMCASE; CHIT2; TSA1902
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC221549).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201653
ORF Size 1428 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201653.2
RefSeq Size 1631 bp
RefSeq ORF 1431 bp
Locus ID 27159
UniProt ID Q9BZP6
Cytogenetics 1p13.2
Protein Families Secreted Protein
Protein Pathways Amino sugar and nucleotide sugar metabolism
MW 52.1 kDa
Summary The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene. provided by RefSeq, Apr 2012
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Documents
Resources
"NM_201653" in other vectors (7)
SKU Description Size Price
RC221549 CHIA (Myc-DDK-tagged)-Human chitinase, acidic (CHIA), transcript variant 4 10 ug
$686.00
RC221549L2 Lenti ORF clone of Human chitinase, acidic (CHIA), transcript variant 4, mGFP tagged 10 ug
$986.00
RC221549L3 Lenti ORF clone of Human chitinase, acidic (CHIA), transcript variant 4, Myc-DDK-tagged 10 ug
$986.00
RC221549L4 Lenti ORF clone of Human chitinase, acidic (CHIA), transcript variant 4, mGFP tagged 10 ug
$986.00
RG221549 CHIA (tGFP-tagged) - Human chitinase, acidic (CHIA), transcript variant 4 10 ug
$886.00
SC308086 CHIA (untagged)-Human chitinase, acidic (CHIA), transcript variant 4 10 ug
$686.00
SC317787 CHIA (untagged)-Human chitinase, acidic (CHIA), transcript variant 4 10 ug
$686.00

Plasmid Map for RC221549L1

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Cloning scheme for RC221549L1

Circular map for RC221549L1

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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