OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

UPF3B (NM_080632) Human Tagged ORF Clone

  • Product Brand Image
Be the first to review this product
SKU
RC221466
UPF3B (Myc-DDK-tagged)-Human UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA343922 Rabbit Polyclonal Anti-UPF3B Antibody
100 ul
$525.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "UPF3B or NM_080632"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UPF3B
Synonyms HUPF3B; MRX62; MRX82; MRXS14; RENT3B; UPF3BP1; UPF3BP2; UPF3BP3; Upf3p-X; UPF3X
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221466 representing NM_080632
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGAAGAGAAGGAGCACAGGCCTAAGGAGAAGCGAGTAACCCTGTTAACCCCCGCCGGGGCCACAG
GCAGCGGTGGTGGGACCTCGGGGGACAGCTCCAAGGGGGAAGATAAGCAGGATCGCAACAAGGAGAAGAA
AGAAGCGCTGAGCAAGGTGGTAATTCGAAGATTACCTCCCACTTTGACCAAGGAGCAGCTTCAGGAACAT
CTTCAACCTATGCCTGAGCATGATTATTTTGAGTTTTTTTCTAATGATACGAGTTTGTATCCTCATATGT
ATGCCAGAGCATACATCAACTTTAAAAACCAAGAGGACATTATTTTGTTCAGGGATCGCTTTGATGGTTA
TGTATTCCTTGACAATAAAGGTCAGGAATATCCCGCTATAGTAGAATTTGCACCTTTTCAAAAAGCTGCA
AAAAAGAAGACTAAGAAAAGAGATACCAAAGTCGGGACTATCGATGATGATCCAGAATATAGAAAGTTTT
TGGAAAGTTATGCCACAGATAATGAGAAAATGACATCTACTCCAGAGACACTGCTAGAGGAAATAGAAGC
AAAAAATAGAGAATTAATAGCTAAAAAGACAACCCCACTTTTGAGCTTCCTGAAAAACAAGCAGAGAATG
AGAGAAGAAAAGAGAGAAGAAAGGAGGAGGAGAGAAATAGAAAGAAAAAGACAAAGAGAAGAAGAGAGGA
GGAAATGGAAAGAAGAAGAGAAACGAAAAAGGAAAGATATAGAAAAGCTAAAGAAGATAGACAGAATTCC
AGAAAGGGACAAATTAAAGGATGAACCAAAGATTAAGGTACACAGGTTTCTGTTACAAGCTGTGAATCAG
AAAAATCTGCTCAAGAAGCCAGAAAAAGGAGATGAAAAAGAATTGGACAAAAGAGAAAAAGCCAAGAAAT
TGGACAAAGAGAATCTCAGTGATGAAAGAGCCAGTGGGCAAAGTTGTACATTGCCCAAGCGTTCTGATAG
CGAACTTAAAGATGAAAAACCAAAGAGACCTGAAGATGAGAGCGGCAGAGACTATAGGGAGAGGGAACGG
GAATATGAACGAGATCAGGAGCGCATACTTCGAGAAAGAGAGAGGCTGAAGCGGCAAGAAGAAGAGCGCC
GTAGGCAGAAGGAGCGCTATGAGAAAGAGAAGACTTTTAAGAGAAAAGAAGAAGAAATGAAAAAAGAGAA
AGACACACTTCGGGATAAAGGAAAGAAGGCTGAAAGTACAGAATCAATAGGCAGCTCAGAAAAAACTGAA
AAGAAAGAAGAAGTGGTCAAGAGAGATCGAATAAGAAACAAGGATCGTCCAGCGATGCAGCTTTACCAAC
CAGGAGCTCGAAGCCGAAATCGACTCTGTCCCCCTGATGACAGCACCAAGTCTGGAGATTCAGCAGCAGA
AAGGAAGCAGGAAAGTGGTATTAGCCATAGAAAAGAAGGAGGAGAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221466 representing NM_080632
Red=Cloning site Green=Tags(s)

MKEEKEHRPKEKRVTLLTPAGATGSGGGTSGDSSKGEDKQDRNKEKKEALSKVVIRRLPPTLTKEQLQEH
LQPMPEHDYFEFFSNDTSLYPHMYARAYINFKNQEDIILFRDRFDGYVFLDNKGQEYPAIVEFAPFQKAA
KKKTKKRDTKVGTIDDDPEYRKFLESYATDNEKMTSTPETLLEEIEAKNRELIAKKTTPLLSFLKNKQRM
REEKREERRRREIERKRQREEERRKWKEEEKRKRKDIEKLKKIDRIPERDKLKDEPKIKVHRFLLQAVNQ
KNLLKKPEKGDEKELDKREKAKKLDKENLSDERASGQSCTLPKRSDSELKDEKPKRPEDESGRDYRERER
EYERDQERILRERERLKRQEEERRRQKERYEKEKTFKRKEEEMKKEKDTLRDKGKKAESTESIGSSEKTE
KKEEVVKRDRIRNKDRPAMQLYQPGARSRNRLCPPDDSTKSGDSAAERKQESGISHRKEGGEE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080632
ORF Size 1449 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080632.3
RefSeq Size 2381 bp
RefSeq ORF 1452 bp
Locus ID 65109
UniProt ID Q9BZI7
Cytogenetics Xq24
MW 57.6 kDa
Summary This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_080632" in other vectors (4)
SKU Description Size Price
RC221466L3 Lenti ORF clone of Human UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC221466L4 Lenti ORF clone of Human UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 1, mGFP tagged 10 ug
$757.00
RG221466 UPF3B (tGFP-tagged) - Human UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 1 10 ug
$657.00
SC309687 UPF3B (untagged)-Human UPF3 regulator of nonsense transcripts homolog B (yeast) (UPF3B), transcript variant 1 10 ug
$503.00

Plasmid Map for RC221466

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221466
Circular map for RC221466

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products