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LRRC15 (NM_130830) Human Tagged ORF Clone

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SKU
RC221437
LRRC15 (Myc-DDK-tagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $811.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRRC15
Synonyms LIB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221437 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACTGAAGCATTATCTCCTTTTGCTGGTGGGCTGCCAAGCCTGGGGTGCAGGGTTGGCCTACCATG
GCTGCCCTAGCGAGTGTACCTGCTCCAGGGCCTCCCAGGTGGAGTGCACCGGGGCACGCATTGTGGCAGT
GCCCACCCCTCTGCCCTGGAACGCCATGAGCCTGCAGATCCTCAACACGCACATCACTGAACTCAATGAG
TCCCCGTTCCTCAATATCTCAGCCCTCATCGCCCTGAGGATTGAGAAGAATGAGCTGTCGCGCATCACGC
CTGGGGCCTTCCGAAACCTGGGCTCGCTGCGCTATCTCAGCCTCGCCAACAACAAGCTGCAGGTTCTGCC
CATCGGCCTCTTCCAGGGCCTGGACAGCCTCGAGTCTCTCCTTCTGTCCAGTAACCAGCTGTTGCAGATC
CAGCCGGCCCACTTCTCCCAGTGCAGCAACCTCAAGGAGCTGCAGTTGCACGGCAACCACCTGGAATACA
TCCCTGACGGAGCCTTCGACCACCTGGTAGGACTCACGAAGCTCAATCTGGGCAAGAATAGCCTCACCCA
CATCTCACCCAGGGTCTTCCAGCACCTGGGCAACCTCCAGGTCCTCCGGCTGTATGAGAACAGGCTCACG
GATATCCCCATGGGCACTTTTGATGGGCTTGTTAACCTGCAGGAACTGGCTCTGCAGCAGAACCAGATTG
GACTGCTCTCCCCTGGTCTCTTCCACAACAACCACAACCTCCAGAGACTCTACCTGTCCAACAACCACAT
CTCCCAGCTGCCACCCAGCGTCTTCATGCAGCTGCCCCAGCTCAACCGTCTTACTCTCTTTGGGAATTCC
CTGAAGGAGCTCTCTCCGGGGATCTTCGGGCCCATGCCCAACCTGCGGGAGCTTTGGCTCTATGACAACC
ACATCTCTTCTCTACCCGACAATGTCTTCAGCAACCTCCGCCAGTTGCAGGTCCTGATTCTTAGCCGCAA
TCAGATCAGCTTCATCTCCCCGGGTGCCTTCAACGGGCTAACGGAGCTTCGGGAGCTGTCCCTCCACACC
AACGCACTGCAGGACCTGGACGGGAACGTCTTCCGCATGTTGGCCAACCTGCAGAACATCTCCCTGCAGA
ACAACCGCCTCAGACAGCTCCCAGGGAATATCTTCGCCAACGTCAATGGCCTCATGGCCATCCAGCTGCA
GAACAACCAGCTGGAGAACTTGCCCCTCGGTATCTTCGATCACCTGGGGAAACTGTGTGAGCTGCGGCTG
TATGACAATCCCTGGAGGTGTGACTCAGACATCCTTCCGCTCCGCAACTGGCTCCTGCTCAACCAGCCTA
GGTTAGGGACGGACACTGTACCTGTGTGTTTCAGCCCAGCCAATGTCCGAGGCCAGTCCCTCATTATCAT
CAATGTCAACGTTGCTGTTCCAAGCGTCCATGTCCCCGAGGTGCCTAGTTACCCAGAAACACCATGGTAC
CCAGACACACCCAGTTACCCTGACACCACATCCGTCTCTTCTACCACTGAGCTAACCAGCCCTGTGGAAG
ACTACACTGATCTGACTACCATTCAGGTCACTGATGACCGCAGCGTTTGGGGCATGACCCAGGCCCAGAG
CGGGCTGGCCATTGCCGCCATTGTAATTGGCATTGTCGCCCTGGCCTGCTCCCTGGCTGCCTGCGTCGGC
TGTTGCTGCTGCAAGAAGAGGAGCCAAGCTGTCCTGATGCAGATGAAGGCACCCAATGAGTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221437 protein sequence
Red=Cloning site Green=Tags(s)

MPLKHYLLLLVGCQAWGAGLAYHGCPSECTCSRASQVECTGARIVAVPTPLPWNAMSLQILNTHITELNE
SPFLNISALIALRIEKNELSRITPGAFRNLGSLRYLSLANNKLQVLPIGLFQGLDSLESLLLSSNQLLQI
QPAHFSQCSNLKELQLHGNHLEYIPDGAFDHLVGLTKLNLGKNSLTHISPRVFQHLGNLQVLRLYENRLT
DIPMGTFDGLVNLQELALQQNQIGLLSPGLFHNNHNLQRLYLSNNHISQLPPSVFMQLPQLNRLTLFGNS
LKELSPGIFGPMPNLRELWLYDNHISSLPDNVFSNLRQLQVLILSRNQISFISPGAFNGLTELRELSLHT
NALQDLDGNVFRMLANLQNISLQNNRLRQLPGNIFANVNGLMAIQLQNNQLENLPLGIFDHLGKLCELRL
YDNPWRCDSDILPLRNWLLLNQPRLGTDTVPVCFSPANVRGQSLIIINVNVAVPSVHVPEVPSYPETPWY
PDTPSYPDTTSVSSTTELTSPVEDYTDLTTIQVTDDRSVWGMTQAQSGLAIAAIVIGIVALACSLAACVG
CCCCKKRSQAVLMQMKAPNEC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_130830
ORF Size 1743 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_130830.5
RefSeq Size 5883 bp
RefSeq ORF 1746 bp
Locus ID 131578
UniProt ID Q8TF66, B3KWI4
Cytogenetics 3q29
Protein Families Transmembrane
MW 64.4 kDa
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Documents
Resources
"NM_130830" in other vectors (6)
SKU Description Size Price
RC221437L1 Lenti-ORF clone of LRRC15 (Myc-DDK-tagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2 10 ug
$1,111.00
RC221437L2 Lenti-ORF clone of LRRC15 (mGFP-tagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2 10 ug
$1,111.00
RC221437L3 Lenti-ORF clone of LRRC15 (Myc-DDK-tagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2 10 ug
$1,111.00
RC221437L4 Lenti-ORF clone of LRRC15 (mGFP-tagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2 10 ug
$1,111.00
RG221437 LRRC15 (tGFP-tagged) - Human leucine rich repeat containing 15 (LRRC15), transcript variant 2 10 ug
$489.00 $1,011.00
SC305914 LRRC15 (untagged)-Human leucine rich repeat containing 15 (LRRC15), transcript variant 2 10 ug
$812.00

Plasmid Map for RC221437

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221437
Circular map for RC221437

file-alt Citations

Fibroblast-expressed LRRC15 is a receptor for SARS-CoV-2 spike and controls antiviral and antifibrotic transcriptional programs
Loo, Waller, Moreno et al
PLoS Biol (2023) 21 (2), e3001967 DOI: 10.1371/journal.pbio.3001967
Cited by 42
AI Summary: OriGene's LRRC15-TurboGFP fusion constructs (RG225990 and RG221437) and LRRC15-myc-DDK fusion constructs (RC225990 and RC221437) were used in this study for flow cytometry, immunoprecipitation, immunocytochemistry, and overexpression experiments to validate the role of LRRC15 as a SARS-CoV-2 spike-binding receptor.
Snippet: …Validation of LRRC15 independent of CRISPR activation LRRC15-TurboGFP fusion constructs (Origene, RG225990 and RG221437) were used for flow cytometry, immunoprecipitation, and immunocytochemistry experiments while LRRC15-myc-DDK fusion constructs (Origene, RC225990 and RC221437) were util;- the inhibitory effect of native overexpression of LRRC15, HEK293T-ACE2-TMPRSS2 cells were transfected with myc-DDK-tagged LRRC15 transcript 1 plasmid (Origene,
FigureFigureFigure
Showing 1-1 of 1 papers.
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