B3GNT8 (NM_198540) Human Tagged ORF Clone

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SKU
RC221160
B3GNT8 (Myc-DDK-tagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (B3GNT8)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol B3GNT8
Synonyms B3GALT7; beta3Gn-T8; BGALT15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221160 representing NM_198540
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCTGCCCCAAGTGCCTTCTCTGCCTGTCAGCACTGCTCACACTCCTGGGCCTCAAAGTGTACATCG
AGTGGACATCCGAGTCCCGGCTCAGCAAGGCCTACCCCAGCCCTCGGGGCACCCCGCCAAGCCCCACGCC
AGCCAACCCTGAGCCCACCCTACCTGCCAACCTCTCCACCCGCCTGGGCCAGACTATCCCGCTGCCCTTT
GCTTACTGGAACCAGCAGCAGTGGCGGCTGGGGTCCCTGCCCAGTGGGGACAGCACTGAAACGGGGGGCT
GCCAGGCTTGGGGGGCCGCCGCCGCCACCGAGATCCCTGACTTCGCCTCCTACCCCAAGGACCTCCGCCG
CTTCTTGCTGTCAGCAGCCTGCCGGAGCTTCCCACAGTGGCTGCCTGGAGGTGGTGGCAGCCAAGTCTCC
AGCTGCTCAGATACTGATGTCCCCTACCTGCTGTTGGCCGTCAAGTCAGAACCAGGGCGCTTTGCAGAAC
GACAGGCCGTGAGAGAGACGTGGGGCAGTCCAGCTCCAGGGATCCGGCTGCTCTTCCTGCTAGGGTCTCC
GGTGGGTGAGGCGGGGCCTGACCTAGACTCACTAGTGGCCTGGGAGAGCCGTCGCTACAGTGACCTGCTG
CTCTGGGACTTCCTCGACGTCCCATTCAACCAGACGCTCAAAGACCTGCTGCTGCTGGCCTGGCTGGGCC
GCCACTGCCCCACCGTGAGTTTTGTCTTGCGAGCTCAGGACGATGCCTTTGTACACACCCCTGCCCTGCT
GGCTCACCTGCGGGCCCTGCCACCTGCCTCGGCCCGAAGCCTCTACCTGGGTGAGGTCTTTACCCAGGCC
ATGCCTCTCCGGAAGCCAGGAGGACCCTTCTATGTGCCCGAGTCCTTCTTCGAAGGTGGCTACCCAGCCT
ATGCAAGCGGGGGTGGCTACGTCATTGCCGGGCGCCTGGCACCCTGGCTGCTGCGGGCGGCAGCCCGTGT
GGCACCCTTCCCCTTTGAGGACGTCTACACTGGCCTTTGCATCCGAGCCCTGGGCCTGGTGCCCCAGGCC
CACCCAGGCTTCCTCACAGCCTGGCCAGCAGACCGCACTGCGGACCACTGTGCTTTCCGCAACCTGCTGC
TGGTACGGCCCCTGGGCCCCCAGGCCAGCATTCGGCTCTGGAAACAACTGCAAGACCCAAGGCTCCAGTG
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC221160 representing NM_198540
Red=Cloning site Green=Tags(s)

MRCPKCLLCLSALLTLLGLKVYIEWTSESRLSKAYPSPRGTPPSPTPANPEPTLPANLSTRLGQTIPLPF
AYWNQQQWRLGSLPSGDSTETGGCQAWGAAAATEIPDFASYPKDLRRFLLSAACRSFPQWLPGGGGSQVS
SCSDTDVPYLLLAVKSEPGRFAERQAVRETWGSPAPGIRLLFLLGSPVGEAGPDLDSLVAWESRRYSDLL
LWDFLDVPFNQTLKDLLLLAWLGRHCPTVSFVLRAQDDAFVHTPALLAHLRALPPASARSLYLGEVFTQA
MPLRKPGGPFYVPESFFEGGYPAYASGGGYVIAGRLAPWLLRAAARVAPFPFEDVYTGLCIRALGLVPQA
HPGFLTAWPADRTADHCAFRNLLLVRPLGPQASIRLWKQLQDPRLQC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198540
ORF Size 1191 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198540.2, NP_940942.1
RefSeq Size 1894 bp
RefSeq ORF 1194 bp
Locus ID 374907
UniProt ID Q7Z7M8
Cytogenetics 19q13.2
MW 43.2 kDa
Summary Beta-1,3-N-acetylglucosaminyltransferase that plays a role in the elongation of specific branch structures of multiantennary N-glycans. Has strong activity towards tetraantennary N-glycans and 2,6 triantennary glycans.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_198540" in other vectors (4)
SKU Description Size Price
RC221160L3 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (B3GNT8), Myc-DDK-tagged 10 ug
$986.00
RC221160L4 Lenti ORF clone of Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (B3GNT8), mGFP tagged 10 ug
$986.00
RG221160 B3GNT8 (tGFP-tagged) - Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (B3GNT8) 10 ug
$886.00
SC307743 B3GNT8 (untagged)-Human UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 (B3GNT8) 10 ug
$732.00

Plasmid Map for RC221160

Download sequence for

Cloning scheme for RC221160

Circular map for RC221160

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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