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SORBS2 (NM_021069) Human Tagged ORF Clone

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SKU
RC221118
SORBS2 (Myc-DDK-tagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $957.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SORBS2
Synonyms ARGBP2; PRO0618
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC221118 representing NM_021069
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTTACTATCAGAGGCCGTTTTCCCCCTCGGCATATTCTCTCCCAGCCTCACTCAACTCCAGCATTG
TCATGCAGCACGGCACATCCCTCGATTCCACAGACACATATCCCCAGCATGCGCAGTCTCTGGATGGCAC
CACCAGCAGCTCTATCCCCCTGTACCGATCCTCAGAGGAAGAGAAGAGAGTGACAGTCATCAAAGCCCCG
CATTACCCAGGGATCGGGCCCGTGGATGAATCCGGAATCCCCACAGCAATTAGAACGACAGTCGACCGGC
CCAAGGACTGGTACAAGACGATGTTTAAGCAAATTCACATGGTGCACAAGCCGGATGATGACACAGACAT
GTATAATACTCCTTATACATACAATGCAGGTCTGTACAACCCACCCTACAGTGCTCAGTCACACCCTGCT
GCAAAGACCCAAACCTACAGACCTCTTTCCAAAAGCCACTCCGACAACAGCCCCAATGCCTTTAAGGATG
CGTCCTCCCCAGTGCCTCCCCCACATGTTCCACCTCCAGTCCCGCCGCTTCGACCAAGAGATCGGTCTTC
AACAGAAAAGCATGACTGGGATCCTCCAGACAGAAAAGTGGACACAAGAAAATTTCGGTCTGAGCCAAGG
AGTATTTTTGAATATGAACCTGGCAAGTCATCAATTCTTCAGCATGAAAGACCAGCCTCCTTGTATCAGT
CCTCTATAGACAGAAGCCTGGAAAGACCCATGAGTTCTGCAAGCATGGCCAGTGACTTCAGGAAGCGGAG
GAAGAGCGAGCCTGCAGTGGGTCCACCACGGGGCTTGGGAGATCAAAGTGCGAGCAGGACTAGCCCAGGC
CGAGTGGACCTCCCAGGATCAAGCACCACTCTTACAAAGTCTTTCACTAGCTCTTCTCCTTCTTCCCCAT
CAAGAGCAAAAGGTGGGGATGATAGCAAAATATGTCCATCCCTTTGCAGTTACTCAGGGCTCAACGGCAA
CCCCTCCAGTGAGTTAGATTACTGTAGCACTTACAGACAGCACTTGGATGTCCCTCGGGACTCACCAAGA
GCCATTAGTTTCAAGAACGGCTGGCAAATGGCCCGGCAAAACGCAGAAATCTGGAGCAGCACGGAAGAAA
CCGTCTCTCCCAAAATCAAATCCCGGAGCTGTGACGATCTCCTAAACGACGACTGCGATAGCTTCCCGGA
CCCGAAAGTCAAGTCGGAAAGCATGGGCTCCCTGTTATGTGAGGAGGACTCCAAGGAGAGCTGCCCCATG
GCGTGGGGGTCCCCCTACGTCCCGGAGGTTCGCAGTAACGGCAGATCAAGGATCAGACACAGGTCAGCCC
GCAACGCCCCGGGGTTCCTGAAGATGTACAAGAAAATGCACCGCATCAACCGCAAGGACCTGATGAACTC
CGAGGTGATCTGCTCCGTGAAGTCCAGGATTCTGCAGTACGAGAGCGAGCAGCAGCACAAGGACCTGCTG
CGCGCCTGGAGCCAGTGCTCCACGGAGGAGGTGCCCCGGGACATGGTGCCCACACGCATCTCCGAATTCG
AAAAGCTGATCCAGAAGTCCAAATCCATGCCAAATTTAGGGGATGACATGCTGTCTCCCGTAACCCTAGA
ACCACCGCAAAATGGCCTATGTCCCAAGAGGAGGTTTTCCATTGAGTATTTGCTGGAGGAAGAAAATCAA
AGCGGCCCCCCCGCTCGGGGCCGGCGAGGCTGCCAGTCTAACGCCCTGGTGCCCATTCACATTGAAGTCA
CCAGCGATGAGCAGCCCCGAGCACACGTGGAGTTTTCCGACAGCGACCAGGACGGGGTTGTGTCCGACCA
CAGTGACTACATTCACCTAGAGGGGTCATCCTTCTGCAGTGAAAGTGACTTTGATCACTTTTCCTTCACA
TCCTCCGAAAGCTTTTACGGATCCAGCCACCACCACCACCATCACCACCACCACCACCACCGCCACCTCA
TCAGCTCCTGCAAAGGCAGGTGCCCGGCCTCCTACACTCGATTTACCACAATGTTAAAACACGAAAGAGC
CAGACACGAAAACACCGAGGAGCCCAGAAGGCAAGAAATGGACCCTGGCCTTTCTAAACTTGCTTTTCTA
GTCAGTCCTGTGCCTTTCCGGAGGAAAAAAAATTCGGCTCCTAAGAAACAGACTGAAAAGGCAAAATGTA
AAGCATCTGTGTTTGAGGCTCTGGACTCTGCCCTTAAAGACATCTGTGACCAAATTAAAGCTGAAAAGAA
AAGGGGGAGCTTGCCGGACAACAGCATCCTGCACCGCCTCATCAGTGAGCTGCTGCCAGATGTTCCCGAG
AGGAACTCATCCCTGAGAGCGCTGAGGAGGAGCCCCCTGCACCAGCCTCTCCACCCACTGCCTCCCGATG
GTGCTATTCATTGTCCACCCTACCAGAATGACTGCGGGAGAATGCCCCGCAGTGCCTCTTTCCAAGACGT
GGACACAGCCAACAGCAGCTGCCACCACCAAGACCGTGGCGGTGCACTCCAAGACCGTGAGTCCCCTAGA
AGTTACTCATCCACTTTGACTGACATGGGGAGAAGTGCACCAAGGGAAAGAAGAGGAACTCCAGAAAAAG
AGAAATTGCCTGCAAAAGCTGTTTATGATTTTAAGGCTCAGACATCTAAGGAGTTGTCATTTAAGAAAGG
AGATACTGTCTACATCCTCAGGAAAATTGATCAAAATTGGTATGAGGGAGAACACCACGGGAGAGTGGGC
ATCTTCCCGATCTCATACGTAGAGAAACTCACACCTCCTGAGAAAGCACAGCCTGCAAGACCACCTCCGC
CAGCCCAGCCCGGAGAAATCGGAGAAGCTATAGCCAAATACAACTTCAACGCAGACACAAATGTGGAGCT
GTCACTGAGAAAGGGAGATAGAGTTATTCTTCTTAAAAGAGTTGATCAAAACTGGTATGAAGGTAAAATC
CCAGGAACCAACAGACAAGGCATCTTCCCTGTTTCCTATGTGGAGGTCGTCAAGAAGAACACAAAAGGTG
CTGAGGACTACCCTGACCCTCCAATACCCCACAGCTATTCTAGTGATAGGATTCACAGCTTGAGCTCAAA
TAAGCCACAGCGTCCTGTGTTTACTCATGAAAATATTCAAGGTGGGGGGGAACCGTTTCAGGCTCTGTAT
AACTATACTCCCAGGAATGAAGATGAGCTGGAGCTCAGAGAAAGTGATGTCATTGATGTCATGGAAAAGT
GTGATGACGGCTGGTTTGTGGGGACCTCAAGAAGAACCAAATTCTTTGGTACTTTCCCCGGAAACTACGT
CAAGAGGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC221118 representing NM_021069
Red=Cloning site Green=Tags(s)

MSYYQRPFSPSAYSLPASLNSSIVMQHGTSLDSTDTYPQHAQSLDGTTSSSIPLYRSSEEEKRVTVIKAP
HYPGIGPVDESGIPTAIRTTVDRPKDWYKTMFKQIHMVHKPDDDTDMYNTPYTYNAGLYNPPYSAQSHPA
AKTQTYRPLSKSHSDNSPNAFKDASSPVPPPHVPPPVPPLRPRDRSSTEKHDWDPPDRKVDTRKFRSEPR
SIFEYEPGKSSILQHERPASLYQSSIDRSLERPMSSASMASDFRKRRKSEPAVGPPRGLGDQSASRTSPG
RVDLPGSSTTLTKSFTSSSPSSPSRAKGGDDSKICPSLCSYSGLNGNPSSELDYCSTYRQHLDVPRDSPR
AISFKNGWQMARQNAEIWSSTEETVSPKIKSRSCDDLLNDDCDSFPDPKVKSESMGSLLCEEDSKESCPM
AWGSPYVPEVRSNGRSRIRHRSARNAPGFLKMYKKMHRINRKDLMNSEVICSVKSRILQYESEQQHKDLL
RAWSQCSTEEVPRDMVPTRISEFEKLIQKSKSMPNLGDDMLSPVTLEPPQNGLCPKRRFSIEYLLEEENQ
SGPPARGRRGCQSNALVPIHIEVTSDEQPRAHVEFSDSDQDGVVSDHSDYIHLEGSSFCSESDFDHFSFT
SSESFYGSSHHHHHHHHHHHRHLISSCKGRCPASYTRFTTMLKHERARHENTEEPRRQEMDPGLSKLAFL
VSPVPFRRKKNSAPKKQTEKAKCKASVFEALDSALKDICDQIKAEKKRGSLPDNSILHRLISELLPDVPE
RNSSLRALRRSPLHQPLHPLPPDGAIHCPPYQNDCGRMPRSASFQDVDTANSSCHHQDRGGALQDRESPR
SYSSTLTDMGRSAPRERRGTPEKEKLPAKAVYDFKAQTSKELSFKKGDTVYILRKIDQNWYEGEHHGRVG
IFPISYVEKLTPPEKAQPARPPPPAQPGEIGEAIAKYNFNADTNVELSLRKGDRVILLKRVDQNWYEGKI
PGTNRQGIFPVSYVEVVKKNTKGAEDYPDPPIPHSYSSDRIHSLSSNKPQRPVFTHENIQGGGEPFQALY
NYTPRNEDELELRESDVIDVMEKCDDGWFVGTSRRTKFFGTFPGNYVKRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_021069
ORF Size 3300 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_021069.5
RefSeq Size 4435 bp
RefSeq ORF 3303 bp
Locus ID 8470
UniProt ID O94875
Cytogenetics 4q35.1
Domains SH3, Sorb
MW 123.9 kDa
Summary Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. This gene encodes the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology (SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_021069" in other vectors (4)
SKU Description Size Price
RC221118L3 Lenti ORF clone of Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2, Myc-DDK-tagged 10 ug
$1,257.00
RC221118L4 Lenti ORF clone of Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2, mGFP tagged 10 ug
$1,257.00
RG221118 SORBS2 (tGFP-tagged) - Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2 10 ug
$1,157.00
SC315181 SORBS2 (untagged)-Human sorbin and SH3 domain containing 2 (SORBS2), transcript variant 2 10 ug
$1,054.00

Plasmid Map for RC221118

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC221118
Circular map for RC221118

file-alt Citations

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