PDE7A (NM_002604) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC220940
PDE7A (Myc-DDK-tagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDE7A
Synonyms HCP1; PDE7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220940 representing NM_002604
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGTGTGTTACCAGCTGCCGGTACTGCCCCTGGACAGGCCGGTCCCCCAGCACGTCCTCAGCCGCC
GAGGAGCCATCAGCTTCAGCTCCAGCTCCGCTCTCTTCGGCTGCCCCAATCCCCGGCAGCTCTCTCAGAG
GCGTGGAGCTATTTCCTATGACAGTTCTGATCAGACTGCATTATACATTCGTATGCTAGGAGATGTACGT
GTAAGGAGCCGAGCAGGATTTGAATCAGAAAGAAGAGGTTCTCACCCATATATTGATTTTCGTATTTTCC
ACTCTCAATCTGAAATTGAAGTGTCTGTCTCTGCAAGGAATATCAGAAGGCTACTAAGTTTCCAGCGATA
TCTTAGATCTTCACGCTTTTTTCGTGGTACTGCGGTTTCAAATTCCCTAAACATTTTAGATGATGATTAT
AATGGACAAGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTATTTGATAGAC
TAACAAATGGAAATAGTCTAGTAAGCTTAACCTTTCATTTATTTAGTCTTCATGGATTAATTGAGTACTT
CCATTTAGATATGATGAAACTTCGTAGATTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCT
TACCATAACGCAGTCCACGCTGCGGATGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTG
CCAATTCTGTAACTCCTTGGGATATCTTGCTGAGCTTAATTGCAGCTGCCACTCATGATCTGGATCATCC
AGGTGTTAATCAACCTTTCCTTATTAAAACTAACCATTACTTGGCAACTTTATACAAGAATACCTCAGTA
CTGGAAAATCACCACTGGAGATCTGCAGTGGGCTTATTGAGAGAATCAGGCTTATTCTCACATCTGCCAT
TAGAAAGCAGGCAACAAATGGAGACACAGATAGGTGCTCTGATACTAGCCACAGACATCAGTCGCCAGAA
TGAGTATCTGTCTTTGTTTAGGTCCCATTTGGATAGAGGTGATTTATGCCTAGAAGACACCAGACACAGA
CATTTGGTTTTACAGATGGCTTTGAAATGTGCTGATATTTGTAACCCATGTCGGACGTGGGAATTAAGCA
AGCAGTGGAGTGAAAAAGTAACGGAGGAATTCTTCCATCAAGGAGATATAGAAAAAAAATATCATTTGGG
TGTGAGTCCACTTTGCGATCGTCACACTGAATCTATTGCCAACATCCAGATTGGTAACTATACATATTTA
GATATAGCTGGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC220940 representing NM_002604
Red=Cloning site Green=Tags(s)

MEVCYQLPVLPLDRPVPQHVLSRRGAISFSSSSALFGCPNPRQLSQRRGAISYDSSDQTALYIRMLGDVR
VRSRAGFESERRGSHPYIDFRIFHSQSEIEVSVSARNIRRLLSFQRYLRSSRFFRGTAVSNSLNILDDDY
NGQAKCMLEKVGNWNFDIFLFDRLTNGNSLVSLTFHLFSLHGLIEYFHLDMMKLRRFLVMIQEDYHSQNP
YHNAVHAADVTQAMHCYLKEPKLANSVTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNTSV
LENHHWRSAVGLLRESGLFSHLPLESRQQMETQIGALILATDISRQNEYLSLFRSHLDRGDLCLEDTRHR
HLVLQMALKCADICNPCRTWELSKQWSEKVTEEFFHQGDIEKKYHLGVSPLCDRHTESIANIQIGNYTYL
DIAG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002604
ORF Size 1272 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002604.2
RefSeq Size 2990 bp
RefSeq ORF 1274 bp
Locus ID 5150
Cytogenetics 8q13.1
Domains HDc, PDEase
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
MW 48.6 kDa
Summary The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. provided by RefSeq, Jul 2011
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_002604" in other vectors (4)
SKU Description Size Price
RC220940L3 Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC220940L4 Lenti ORF clone of Human phosphodiesterase 7A (PDE7A), transcript variant 2, mGFP tagged 10 ug
$757.00
RG220940 PDE7A (tGFP-tagged) - Human phosphodiesterase 7A (PDE7A), transcript variant 2 10 ug
$489.00 $657.00
SC313399 PDE7A (untagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 2 10 ug
$457.00

Plasmid Map for RC220940

Download sequence for

Cloning scheme for RC220940

Circular map for RC220940

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products