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NFE4 (NM_001085386) Human Tagged ORF Clone

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SKU
RC220641
NFE4 (Myc-DDK-tagged)-Human transcription factor NF-E4 (NFE4)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "NFE4 or NM_001085386"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NFE4
Synonyms NF-E4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220641 representing NM_001085386
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

CTGCCTCGTGTTGTCTGTTGGCACACTCTCAAGAGTTTGAACGGATACAAGAATCTTTCATCTGGTGCCG
AAACCCGGGAGGGGCTCCGGTCTTCGTCCCCCGTGGACCTACCCCTCCGCCCCAGAAAGCAGGCCACAGC
AGCCGGACAAAGGAAGCTCCTCAGCCTCCAGTTGCTTCTCTGTGCATGCACATCAGTCACTGATCTCACC
TACTGGGGCCCTGCAGGCCATGGGGCCACAGCTCCACACAGAAGCCTCCTAGCAATCCACCTCCACCTGG
TGCCTGCTTCAAGTGCGGCAATGAAGGCCACTGGCCCACACAATGCCCAAACCCAGGTAAACCCACGAGG
CCATGCCCCCTCTGCGGAGGACCCCACTGGAAGTTGGACTGTGAGCGGCCCCTGCAAGGACCACCCCCAT
CCCTTCCTGAGCCAATCAAACCCTCCTACTCGGATCTCGTCAGCCTTGCCGCTGAAGACTGATAGTGCCT
TGGAACAGACACCCCAGCAACTACCATCGCTTCATCTGAGCCAAGGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220641 protein sequence
Red=Cloning site Green=Tags(s)

LPRVVCWHTLKSLNGYKNLSSGAETREGLRSSSPVDLPLRPRKQATAAGQRKLLSLQLLLCACTSVTDLT
YWGPAGHGATAPHRSLLAIHLHLVPASSAAMKATGPHNAQTQVNPRGHAPSAEDPTGSWTVSGPCKDHPH
PFLSQSNPPTRISSALPLKTDSALEQTPQQLPSLHLSQG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_001085386
ORF Size 537 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 967 bp
RefSeq ORF 540 bp
Locus ID 58160
Cytogenetics 7q22.1
MW 20.33 kDa
Summary The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. provided by RefSeq, Jul 2008
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"NM_001085386" in other vectors (4)
SKU Description Size Price
RC220641L3 Lenti ORF clone of Human transcription factor NF-E4 (NFE4), Myc-DDK-tagged 10 ug
$600.00
RC220641L4 Lenti ORF clone of Human transcription factor NF-E4 (NFE4), mGFP tagged 10 ug
$600.00
RG220641 NFE4 (tGFP-tagged) - Human transcription factor NF-E4 (NFE4) 10 ug
$500.00
SC316149 NFE4 (untagged)-Human transcription factor NF-E4 (NFE4) 10 ug
$330.00

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