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CDCP1 (NM_022842) Human Tagged ORF Clone

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SKU
RC220633
CDCP1 (Myc-DDK-tagged)-Human CUB domain containing protein 1 (CDCP1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $765.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDCP1
Synonyms CD318; SIMA135; TRASK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220633 representing NM_022842
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCCTGAACTGCGGGGTCTCTATCGCACTGCTAGGGGTTCTGCTGCTGGGTGCGGCGCGCCTGC
CGCGCGGGGCAGAAGCTTTTGAGATTGCTCTGCCACGAGAAAGCAACATTACAGTTCTCATAAAGCTGGG
GACCCCGACTCTGCTGGCAAAACCCTGTTACATCGTCATTTCTAAAAGACATATAACCATGTTGTCCATC
AAGTCTGGAGAAAGAATAGTCTTTACCTTTAGCTGCCAGAGTCCTGAGAATCACTTTGTCATAGAGATCC
AGAAAAATATTGACTGTATGTCAGGCCCATGTCCTTTTGGGGAGGTTCAGCTTCAGCCCTCGACATCGTT
GTTGCCTACCCTCAACAGAACTTTCATCTGGGATGTCAAAGCTCATAAGAGCATCGGTTTAGAGCTGCAG
TTTTCCATCCCTCGCCTGAGGCAGATCGGTCCGGGTGAGAGCTGCCCAGACGGAGTCACTCACTCCATCA
GCGGCCGAATCGATGCCACCGTGGTCAGGATCGGAACCTTCTGCAGCAATGGCACTGTGTCCCGGATCAA
GATGCAAGAAGGAGTGAAAATGGCCTTACACCTCCCATGGTTCCACCCCAGAAATGTCTCCGGCTTCAGC
ATTGCAAACCGCTCATCTATAAAACGTCTGTGCATCATCGAGTCTGTGTTTGAGGGTGAAGGCTCAGCAA
CCCTGATGTCTGCCAACTACCCAGAAGGCTTCCCTGAGGATGAGCTCATGACGTGGCAGTTTGTCGTTCC
TGCACACCTGCGGGCCAGCGTCTCCTTCCTCAACTTCAACCTCTCCAACTGTGAGAGGAAGGAGGAGCGG
GTTGAATACTACATCCCGGGCTCCACCACCAACCCCGAGGTGTTCAAGCTGGAGGACAAGCAGCCTGGGA
ACATGGCGGGGAACTTCAACCTCTCTCTGCAAGGCTGTGACCAAGATGCCCAAAGTCCAGGGATCCTCCG
GCTGCAGTTCCAAGTTTTGGTCCAACATCCACAAAATGAAAGCAATAAAATCTACGTGGTTGACTTGAGT
AATGAGCGAGCCATGTCACTCACCATCGAGCCACGGCCCGTCAAACAGAGCCGCAAGTTTGTCCCTGGCT
GTTTCGTGTGTCTAGAATCTCGGACCTGCAGTAGCAACCTCACCCTGACATCTGGCTCCAAACACAAAAT
CTCCTTCCTTTGTGATGATCTGACACGTCTGTGGATGAATGTGGAAAAAACCATAAGCTGCACAGACCAC
CGGTACTGCCAAAGGAAATCCTACTCACTCCAGGTGCCCAGTGACATCCTCCACCTGCCTGTGGAGCTGC
ATGACTTCTCCTGGAAGCTGCTGGTGCCCAAGGACAGGCTCAGCCTGGTGCTGGTGCCAGCCCAGAAGCT
GCAGCAGCATACACACGAGAAGCCCTGCAACACCAGCTTCAGCTACCTCGTGGCCAGTGCCATACCCAGC
CAGGACCTGTACTTCGGCTCCTTCTGCCCGGGAGGCTCTATCAAGCAGATCCAGGTGAAGCAGAACATCT
CGGTGACCCTTCGCACCTTTGCCCCCAGCTTCCGACAAGAGGCCTCCAGGCAGGGTCTGACGGTGTCCTT
TATACCTTATTTCAAAGAGGAAGGCGTTTTCACGGTGACCCCTGACACAAAAAGCAAGGTCTACCTGAGG
ACCCCCAACTGGGACCGGGGCCTGCCATCCCTCACCTCTGTGTCCTGGAACATCAGCGTGCCCAGAGACC
AGGTGGCCTGCCTGACTTTCTTTAAGGAGCGGAGCGGCGTGGTCTGCCAGACAGGGCGCGCATTCATGAT
CATCCAGGAGCAGCGGACCCGGGCTGAGGAGATCTTCAGCCTGGACGAGGATGTGCTCCCCAAGCCAAGC
TTCCACCATCACAGCTTCTGGGTCAACATCTCTAACTGCAGCCCCACGAGCGGCAAGCAGCTAGACCTGC
TCTTCTCGGTGACACTTACCCCAAGGACTGTGGACTTGACTGTCATCCTCATCGCAGCGGTGGGAGGTGG
AGTCTTACTGCTGTCTGCCCTCGGGCTCATCATTTGCTGTGTGAAAAAGAAGAAAAAGAAGACAAACAAG
GGCCCCGCTGTGGGTATCTACAATGGCAACATCAATACTGAGATGCCGAGGCAGCCAAAAAAGTTTCAGA
AAGGGCGAAAGGACAATGACTCCCATGTGTATGCAGTCATCGAGGACACCATGGTATATGGGCATCTGCT
ACAGGATTCCAGCGGCTCCTTCCTGCAGCCAGAGGTGGACACCTACCGGCCGTTCCAGGGCACCATGGGG
GTCTGTCCTCCCTCCCCACCCACCATATGCTCCAGGGCCCCAACTGCAAAGTTGGCCACTGAGGAGCCAC
CTCCTCGCTCCCCTCCTGAGTCTGAGAGTGAACCGTACACCTTCTCCCATCCCAACAATGGGGATGTAAG
CAGCAAGGACACAGACATTCCCTTACTGAACACTCAGGAGCCCATGGAGCCAGCAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220633 representing NM_022842
Red=Cloning site Green=Tags(s)

MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSI
KSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQ
FSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFS
IANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEER
VEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLS
NERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDH
RYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPS
QDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFRQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLR
TPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPS
FHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNK
GPAVGIYNGNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMG
VCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022842
ORF Size 2508 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022842.5
RefSeq Size 6017 bp
RefSeq ORF 2511 bp
Locus ID 64866
UniProt ID Q9H5V8
Cytogenetics 3p21.31
Protein Families ES Cell Differentiation/IPS, Transmembrane
MW 92.8 kDa
Summary This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. provided by RefSeq, May 2013
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"NM_022842" in other vectors (6)
SKU Description Size Price
RC220633L1 Lenti ORF clone of Human CUB domain containing protein 1 (CDCP1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,065.00
RC220633L2 Lenti ORF clone of Human CUB domain containing protein 1 (CDCP1), transcript variant 1, mGFP tagged 10 ug
$1,065.00
RC220633L3 Lenti ORF clone of Human CUB domain containing protein 1 (CDCP1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,065.00
RC220633L4 Lenti ORF clone of Human CUB domain containing protein 1 (CDCP1), transcript variant 1, mGFP tagged 10 ug
$1,065.00
RG220633 CDCP1 (tGFP-tagged) - Human CUB domain containing protein 1 (CDCP1), transcript variant 1 10 ug
$789.00 $965.00
SC305059 CDCP1 (untagged)-Human CUB domain containing protein 1 (CDCP1), transcript variant 1 10 ug
$766.00

Plasmid Map for RC220633

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220633
Circular map for RC220633

file-alt Citations

FBXL14 abolishes breast cancer progression by targeting CDCP1 for proteasomal degradation
Cui, Kim, Lee et al
Oncogene (2018)
AI Summary: OriGene’s pCMV6-AC-CDCP1 mammalian expression vector was used in this study to investigate the role of CDCP1 in breast cancer progression and its regulation by FBXL14.
Snippet: Mammalian expression vectors pCMV6-AC-CDCP1 (OriGene, Korea);pCMV6 empty vector, pCMV6-FBXL14 and pCMV6-STAT3 were kindly provided by Hee Yong Chung (Department of Medicine, Hanyang University, Korea). His-Ubiquitin DNA plasmid was gifted by Seunghee Bae (Konkuk University, Korea)
Glucocorticoids and histone deacetylase inhibitors cooperate to block the invasiveness of basal-like breast cancer cells through novel mechanisms
Law, Corsino, Jahn et al
Oncogene (2013) 32 (10), 1316-29 DOI: 10.1038/onc.2012.138
Cited by 81
AI Summary: OriGene contributed to this study by providing the human CDCP1 cDNA clone (cat. # RC220633), which was used to construct adenoviral vectors for investigating the role of CDCP1 in breast cancer cell invasiveness.
Snippet: 6 . Construction of CDCP1 adenoviral vectors. cDNA encoding human CDCP1 (accession no. NM_002842) cloned into the SgfI and MluI sites of pCMV6 were purchased from Origene (Rockville, MD, USA) (cat. # RC220633).
Showing 1-2 of 2 papers.
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