SIDT2 (NM_001040455) Human Tagged ORF Clone
$589.00
$762.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | SIDT2 |
| Synonyms | CGI-40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001040455 |
| ORF Size | 2496 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001040455.2 |
| RefSeq Size | 3919 bp |
| RefSeq ORF | 2499 bp |
| Locus ID | 51092 |
| UniProt ID | Q8NBJ9 |
| Cytogenetics | 11q23.3 |
| Protein Families | Transmembrane |
| MW | 94.3 kDa |
| Summary | Mediates the translocation of RNA and DNA across the lysosomal membrane during RNA and DNA autophagy (RDA), a process in which RNA or DNA is directly imported into lysosomes in an ATP-dependent manner, and degraded (PubMed:27046251, PubMed:27846365). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis (PubMed:28277980). Involved in the uptake of single-stranded oligonucleotides by living cells, a process called gymnosis. In vitro, mediates the uptake of linear DNA more efficiently than that of circular DNA, but exhibits similar uptake efficacy toward RNA and DNA. Binds long double-stranded RNA (dsRNA) (500 - 700 base pairs), but not dsRNA shorter than 100 bp (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001040455" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC220528L3 | Lenti-ORF clone of SIDT2 (Myc-DDK-tagged)-Human SID1 transmembrane family, member 2 (SIDT2) | 10 ug |
$1,062.00
|
|
| RC220528L4 | Lenti-ORF clone of SIDT2 (mGFP-tagged)-Human SID1 transmembrane family, member 2 (SIDT2) | 10 ug |
$1,062.00
|
|
| RG220528 | SIDT2 (tGFP-tagged) - Human SID1 transmembrane family, member 2 (SIDT2) | 10 ug |
$789.00
$962.00
|
|
| SC316840 | SIDT2 (untagged)-Human SID1 transmembrane family, member 2 (SIDT2) | 10 ug |
$763.00
|
|
Citations
A C. elegans model of human α1-antitrypsin deficiency links components of the RNAi pathway to misfolded protein turnover
Long, Benson, Kwak et al
Hum. Mol. Genet. (2014)
Long, Benson, Kwak et al
Hum. Mol. Genet. (2014)
Cited by 48
AI Summary: OriGene's SIDT1 and SIDT2 expression vectors were used in this study to investigate the role of these proteins in misfolded protein turnover related to α1-antitrypsin deficiency.
Snippet: Small interfering RNAs (siRNAs) against SIDT1 and 2 as well as SIDT1/2 expression vectors were transfected with Lipofectamine 2000 (Invitrogen, Carlsbad, CA) following the manufacturer’s instructions. SIDT siRNAs were purchased from QIAGEN. SIDT1/2 vectors were purchased from Origene. Antibodies to SIDT2 were purchased from Abcam
Showing 1-1 of 1 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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