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TMEM185A (NM_032508) Human Tagged ORF Clone

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SKU
RC220454
TMEM185A (Myc-DDK-tagged)-Human transmembrane protein 185A (TMEM185A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM185A
Synonyms CXorf13; ee3; FAM11A; FRAXF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220454 representing NM_032508
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCTGAGGGGCCTCTTCCAGGACTTCAACCCGAGTAAATTCCTCATCTATGCCTGTCTGCTGCTGT
TCTCTGTGCTGCTGGCCCTTCGTTTGGATGGCATCATACAGTGGAGTTACTGGGCTGTCTTTGCTCCAAT
ATGGCTGTGGAAGTTAATGGTCATTGTTGGAGCCTCAGTTGGAACTGGAGTCTGGGCACGAAATCCTCAA
TATCGAGCAGAAGGAGAAACGTGTGTGGAGTTTAAAGCCATGTTGATTGCAGTGGGCATCCACTTGCTCT
TGTTGATGTTTGAAGTTCTGGTCTGTGACAGAATCGAGAGAGGAAGCCATTTCTGGCTCCTGGTCTTCAT
GCCGCTGTTCTTTGTTTCCCCGGTGTCTGTTGCAGCTTGCGTTTGGGGCTTTCGACATGACAGGTCACTA
GAGTTAGAAATCCTGTGTTCTGTCAACATTCTCCAGTTTATATTCATTGCCTTAAGACTGGACAAGATCA
TCCACTGGCCCTGGCTTGTTGTGTGTGTCCCGCTGTGGATTCTCATGTCCTTTCTGTGCCTGGTGGTCCT
CTACTACATTGTGTGGTCCGTCTTGTTCTTGCGCTCTATGGATGTGATTGCGGAGCAGCGCAGGACACAC
ATAACCATGGCCCTGAGCTGGATGACCATCGTCGTGCCCCTTCTTACATTTGAGATTCTGCTGGTTCACA
AACTGGATGGCCACAACGCCTTCTCCTGCATCCCGATCTTTGTCCCCCTTTGGCTCTCGTTGATCACGCT
GATGGCAACCACATTTGGACAGAAGGGAGGAAACCACTGGTGGTTTGGTATCCGCAAAGATTTCTGTCAG
TTTCTGCTTGAAATCTTCCCATTTCTACGAGAATATGGAAACATTTCCTATGATCTCCATCACGAAGATA
ATGAAGAAACCGAAGAGACCCCAGTTCCGGAGCCCCCTAAAATCGCACCCATGTTTCGAAAGAAGGCCAG
GGTGGTCATTACCCAGAGCCCTGGGAAGTATGTGCTCCCACCTCCCAAATTAAATATCGAAATGCCAGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220454 representing NM_032508
Red=Cloning site Green=Tags(s)

MNLRGLFQDFNPSKFLIYACLLLFSVLLALRLDGIIQWSYWAVFAPIWLWKLMVIVGASVGTGVWARNPQ
YRAEGETCVEFKAMLIAVGIHLLLLMFEVLVCDRIERGSHFWLLVFMPLFFVSPVSVAACVWGFRHDRSL
ELEILCSVNILQFIFIALRLDKIIHWPWLVVCVPLWILMSFLCLVVLYYIVWSVLFLRSMDVIAEQRRTH
ITMALSWMTIVVPLLTFEILLVHKLDGHNAFSCIPIFVPLWLSLITLMATTFGQKGGNHWWFGIRKDFCQ
FLLEIFPFLREYGNISYDLHHEDNEETEETPVPEPPKIAPMFRKKARVVITQSPGKYVLPPPKLNIEMPD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032508
ORF Size 1050 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032508.4
RefSeq Size 2850 bp
RefSeq ORF 1053 bp
Locus ID 84548
UniProt ID Q8NFB2
Cytogenetics Xq28
Protein Families Transmembrane
MW 40.5 kDa
Summary The protein encoded by this gene is predicted to be a transmembrane protein. This gene is best known for localizing to the CpG island of the fragile site FRAXF. The 5' untranslated region of this gene contains a CGG trinucleotide repeat sequence that normally consists of 7-40 tandem CGG repeats but which can expand to greater than 300 repeats. Methylation of the CpG island leads to transcriptional silencing of this gene, but neither the silencing nor an expanded repeat region appear to manifest itself in a clear phenotypic manner. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been defined on the X chromosome. provided by RefSeq, Aug 2013
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"NM_032508" in other vectors (4)
SKU Description Size Price
RC220454L3 Lenti ORF clone of Human transmembrane protein 185A (TMEM185A), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC220454L4 Lenti ORF clone of Human transmembrane protein 185A (TMEM185A), transcript variant 1, mGFP tagged 10 ug
$757.00
RG220454 TMEM185A (tGFP-tagged) - Human transmembrane protein 185A (TMEM185A), transcript variant 1 10 ug
$489.00 $657.00
SC127146 TMEM185A (untagged)-Human transmembrane protein 185A (TMEM185A), transcript variant 1 10 ug
$457.00

Plasmid Map for RC220454

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220454
Circular map for RC220454

file-alt Citations

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