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PDE11A (NM_016953) Human Tagged ORF Clone

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SKU
RC220304
PDE11A (Myc-DDK-tagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $855.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDE11A
Synonyms PPNAD2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220304 representing NM_016953
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCTCCCGCCTGGACTTTGGGGAGGTGGAAACTTTCCTGGACAGGCACCCAGAGTTGTTTGAAG
ATTACTTGATGCGGAAGGGGAAGCAGGAGATGGTTGAAAAGTGGCTGCAGAGGCACAGTCAGGGTCAGGG
GGCTTTAGGTCCAAGGCCCTCTTTGGCTGGTACCAGCAGCTTGGCTCACAGCACCTGCAGAGGTGGCAGC
AGCGTTGGTGGTGGCACTGGACCAAATGGCTCTGCCCACAGCCAGCCCCTTCCCGGTGGCGGGGACTGTG
GTGGGGTTCCCTTGAGTCCCAGCTGGGCCGGTGGCAGCAGGGGCGATGGGAACCTGCAGCGGAGAGCTTC
TCAGAAAGAGCTAAGGAAGAGTTTTGCCCGCTCCAAGGCCATCCACGTGAACAGGACCTACGATGAACAG
GTGACCTCCCGGGCTCAGGAACCCCTGAGTAGTGTACGACGGAGGGCACTTCTCCGGAAGGCAAGCTCCC
TGCCCCCCACCACAGCCCATATTCTCAGTGCGCTGCTGGAATCGAGAGTGAATCTGCCTCAGTATCCCCC
TACAGCCATCGACTACAAGTGCCATCTGAAAAAGCATAATGAGCGTCAGTTCTTTCTGGAATTGGTCAAA
GATATCTCCAATGACCTTGACCTCACCAGCCTGAGCTACAAGATTCTCATCTTTGTCTGCCTTATGGTGG
ATGCTGACCGCTGCTCTCTTTTCCTGGTGGAAGGGGCAGCTGCTGGCAAGAAGACCTTGGTCTCCAAATT
CTTTGATGTGCATGCAGGAACCCCTCTGCTGCCTTGCAGCAGCACAGAGAACTCAAATGAGGTGCAGGTC
CCCTGGGGCAAAGGTATCATTGGCTATGTCGGGGAGCATGGAGAAACGGTCAACATTCCTGATGCCTACC
AGGATCGACGATTCAATGATGAAATCGACAAGCTAACTGGATACAAGACAAAATCATTATTGTGCATGCC
TATCCGAAGCAGTGATGGTGAGATTATTGGTGTGGCCCAAGCGATAAATAAGATTCCTGAAGGAGCTCCA
TTTACTGAAGATGATGAAAAAGTTATGCAGATGTATCTTCCATTTTGTGGAATCGCCATATCTAACGCTC
AGCTCTTTGCTGCCTCAAGGAAAGAATATGAAAGAAGCAGAGCTTTGCTAGAGGTGGTTAATGACCTCTT
TGAAGAACAGACTGACCTGGAGAAAATTGTCAAGAAAATAATGCATCGGGCCCAAACTCTGCTGAAATGT
GAACGCTGTTCTGTTTTACTCCTAGAGGACATCGAATCACCAGTGGTGAAATTTACCAAATCCTTTGAAT
TGATGTCCCCAAAGTGCAGTGCTGATGCTGAGAACAGTTTCAAAGAAAGCATGGAGAAATCATCATACTC
CGACTGGCTAATAAATAACAGCATTGCTGAGCTGGTTGCTTCAACAGGCCTTCCAGTGAACATCAGTGAT
GCCTACCAGGATCCGCGCTTTGATGCAGAGGCAGACCAGATATCTGGTTTTCACATAAGATCTGTTCTTT
GTGTCCCTATTTGGAATAGCAACCACCAAATAATTGGAGTGGCTCAAGTGTTAAACAGACTTGATGGGAA
ACCTTTTGATGATGCAGATCAACGACTTTTTGAGGCTTTTGTCATCTTTTGTGGACTTGGCATCAACAAC
ACAATTATGTATGATCAAGTGAAGAAGTCCTGGGCCAAGCAGTCTGTGGCTCTTGATGTGCTATCATACC
ATGCAACATGTTCAAAAGCTGAAGTTGACAAGTTTAAGGCAGCCAACATCCCTCTGGTGTCAGAACTTGC
CATCGATGACATTCATTTTGATGACTTTTCTCTCGACGTTGATGCCATGATCACAGCTGCTCTCCGGATG
TTCATGGAGCTGGGGATGGTACAGAAATTTAAAATTGACTATGAGACACTGTGTAGGTGGCTTTTGACAG
TGAGGAAAAACTATCGGATGGTTCTATACCACAACTGGAGACATGCCTTCAACGTGTGTCAGCTGATGTT
CGCGATGTTAACCACTGCTGGGTTTCAAGACATTCTGACCGAGGTGGAAATTTTAGCGGTGATTGTGGGA
TGCCTGTGTCATGACCTCGACCACAGGGGAACCAACAATGCCTTCCAAGCTAAGAGTGGCTCTGCCCTGG
CCCAACTCTATGGAACCTCTGCTACCTTGGAGCATCACCATTTCAACCACGCCGTGATGATCCTTCAAAG
TGAGGGTCACAATATCTTTGCTAACCTGTCCTCCAAGGAATATAGTGACCTTATGCAGCTTTTGAAGCAG
TCAATATTGGCAACAGACCTCACGCTGTACTTTGAGAGGAGAACTGAATTCTTTGAACTTGTCAGTAAAG
GAGAATACGATTGGAACATCAAAAACCATCGTGATATATTTCGATCAATGTTAATGACAGCCTGTGACCT
TGGAGCCGTGACCAAACCGTGGGAGATCTCCAGACAGGTGGCAGAACTTGTAACCAGTGAGTTCTTCGAA
CAAGGAGATCGGGAGAGATTAGAGCTCAAACTCACTCCTTCAGCAATTTTTGATCGGAACCGGAAGGATG
AACTGCCTCGGTTGCAACTGGAGTGGATTGATAGCATCTGCATGCCTTTGTATCAGGCACTGGTGAAGGT
CAACGTGAAACTGAAGCCGATGCTAGATTCAGTAGCTACAAACAGAAGTAAGTGGGAAGAGCTACACCAA
AAACGACTGCTGGCCTCAACTGCCTCATCCTCCCCTGCCAGTGTTATGGTAGCCAAGGAAGACAGGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220304 representing NM_016953
Red=Cloning site Green=Tags(s)

MAASRLDFGEVETFLDRHPELFEDYLMRKGKQEMVEKWLQRHSQGQGALGPRPSLAGTSSLAHSTCRGGS
SVGGGTGPNGSAHSQPLPGGGDCGGVPLSPSWAGGSRGDGNLQRRASQKELRKSFARSKAIHVNRTYDEQ
VTSRAQEPLSSVRRRALLRKASSLPPTTAHILSALLESRVNLPQYPPTAIDYKCHLKKHNERQFFLELVK
DISNDLDLTSLSYKILIFVCLMVDADRCSLFLVEGAAAGKKTLVSKFFDVHAGTPLLPCSSTENSNEVQV
PWGKGIIGYVGEHGETVNIPDAYQDRRFNDEIDKLTGYKTKSLLCMPIRSSDGEIIGVAQAINKIPEGAP
FTEDDEKVMQMYLPFCGIAISNAQLFAASRKEYERSRALLEVVNDLFEEQTDLEKIVKKIMHRAQTLLKC
ERCSVLLLEDIESPVVKFTKSFELMSPKCSADAENSFKESMEKSSYSDWLINNSIAELVASTGLPVNISD
AYQDPRFDAEADQISGFHIRSVLCVPIWNSNHQIIGVAQVLNRLDGKPFDDADQRLFEAFVIFCGLGINN
TIMYDQVKKSWAKQSVALDVLSYHATCSKAEVDKFKAANIPLVSELAIDDIHFDDFSLDVDAMITAALRM
FMELGMVQKFKIDYETLCRWLLTVRKNYRMVLYHNWRHAFNVCQLMFAMLTTAGFQDILTEVEILAVIVG
CLCHDLDHRGTNNAFQAKSGSALAQLYGTSATLEHHHFNHAVMILQSEGHNIFANLSSKEYSDLMQLLKQ
SILATDLTLYFERRTEFFELVSKGEYDWNIKNHRDIFRSMLMTACDLGAVTKPWEISRQVAELVTSEFFE
QGDRERLELKLTPSAIFDRNRKDELPRLQLEWIDSICMPLYQALVKVNVKLKPMLDSVATNRSKWEELHQ
KRLLASTASSSPASVMVAKEDRN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016953
ORF Size 2799 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016953.4
RefSeq Size 9305 bp
RefSeq ORF 2802 bp
Locus ID 50940
UniProt ID Q9HCR9
Cytogenetics 2q31.2
Domains GAF, HDc, PDEase
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
MW 104.6 kDa
Summary The 3',5'-cyclic nucleotides cAMP and cGMP function as second messengers in a wide variety of signal transduction pathways. 3',5'-cyclic nucleotide phosphodiesterases (PDEs) catalyze the hydrolysis of cAMP and cGMP to the corresponding 5'-monophosphates and provide a mechanism to downregulate cAMP and cGMP signaling. This gene encodes a member of the PDE protein superfamily. Mutations in this gene are a cause of Cushing disease and adrenocortical hyperplasia. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_016953" in other vectors (6)
SKU Description Size Price
RC220304L1 Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, Myc-DDK-tagged 10 ug
$1,155.00
RC220304L2 Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, mGFP tagged 10 ug
$1,155.00
RC220304L3 Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, Myc-DDK-tagged 10 ug
$1,155.00
RC220304L4 Lenti ORF clone of Human phosphodiesterase 11A (PDE11A), transcript variant 4, mGFP tagged 10 ug
$1,155.00
RG220304 PDE11A (tGFP-tagged) - Human phosphodiesterase 11A (PDE11A), transcript variant 4 10 ug
$1,055.00
SC125346 PDE11A (untagged)-Human phosphodiesterase 11A (PDE11A), transcript variant 4 10 ug
$856.00

Plasmid Map for RC220304

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220304
Circular map for RC220304

file-alt Citations

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