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SLC30A10 (NM_018713) Human Tagged ORF Clone

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SKU
RC220219
SLC30A10 (Myc-DDK-tagged)-Human solute carrier family 30, member 10 (SLC30A10)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC30A10
Synonyms HMDPC; HMNDYT1; ZnT-10; ZNT8; ZNT10; ZRC1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220219 representing NM_018713
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCGCTACTCTGGCAAGACGTGCCGGCTGCTCTTCATGCTGGTGCTCACCGTCGCCTTCTTCGTGG
CGGAGCTGGTCTCCGGCTACCTGGGCAACTCCATCGCGCTGCTCTCCGACTCCTTCAACATGCTCTCCGA
CCTGATCTCGCTGTGCGTGGGCCTGAGCGCCGGCTACATCGCCCGGCGCCCCACCCGGGGCTTCAGCGCC
ACCTACGGCTACGCCCGCGCCGAGGTGGTGGGCGCGCTGAGCAACGCGGTCTTCCTCACCGCGCTCTGCT
TCACCATCTTCGTGGAGGCCGTGCTGCGCCTGGCCCGGCCCGAGCGCATCGATGACCCCGAGCTGGTGCT
CATCGTCGGCGTCCTGGGGCTGTTGGTCAACGTGGTGGGGCTGCTCATCTTCCAGGACTGCGCCGCCTGG
TTCGCGTGCTGCCTCCGGGGACGCAGTCGCCGCCTGCAGCAGCGGCAGCAGCTGGCGGAGGGCTGTGTCC
CCGGCGCTTTCGGGGGGCCTCAGGGCGCGGAGGACCCGCGGCGCGCGGCGGACCCGACAGCCCCAGGCTC
GGACTCGGCCGTAACCCTCCGGGGGACCTCGGTGGAAAGGAAGCGGGAGAAGGGGGCGACCGTGTTCGCA
AACGTAGCAGGTGATTCCTTCAACACCCAGAATGAGCCAGAAGACATGATGAAAAAAGAGAAAAAGTCTG
AAGCTCTGAATATCAGAGGTGTACTTTTGCATGTGATGGGAGATGCCCTGGGGTCCGTGGTTGTGGTCAT
CACGGCCATCATATTCTATGTGCTTCCCCTGAAGAGTGAGGACCCGTGTAACTGGCAGTGTTACATTGAC
CCCAGCCTGACTGTCCTCATGGTCATCATCATTTTGTCATCTGCCTTCCCGCTTATCAAGGAGACCGCTG
CCATTCTGCTACAGATGGTCCCAAAAGGAGTCAACATGGAAGAGCTGATGAGTAAACTCTCTGCTGTGCC
TGGAATTAGCAGTGTACATGAAGTGCACATCTGGGAACTTGTAAGTGGAAAGATTATTGCCACCCTGCAC
ATCAAGTATCCTAAGGACAGGGGATATCAAGATGCCAGCACAAAAATTCGAGAAATCTTCCACCATGCGG
GAATCCACAATGTGACCATCCAGTTTGAAAATGTGGACTTGAAGGAACCCCTGGAGCAGAAGGACTTACT
GTTGCTCTGCAACTCACCCTGCATCTCCAAGGGCTGTGCTAAGCAGCTGTGTTGTCCCCCCGGGGCACTG
CCTCTGGCTCACGTCAATGGCTGTGCTGAGCACAATGGTGGGCCCTCTCTAGACACATACGGAAGTGATG
GCCTCAGTAGAAGAGACGCAAGAGAAGTGGCTATTGAAGTGTCTTTGGATAGCTGTCTGAGTGACCACGG
ACAAAGTCTTAACAAAACTCAGGAGGACCAATGTTATGTCAACAGAACGCATTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220219 representing NM_018713
Red=Cloning site Green=Tags(s)

MGRYSGKTCRLLFMLVLTVAFFVAELVSGYLGNSIALLSDSFNMLSDLISLCVGLSAGYIARRPTRGFSA
TYGYARAEVVGALSNAVFLTALCFTIFVEAVLRLARPERIDDPELVLIVGVLGLLVNVVGLLIFQDCAAW
FACCLRGRSRRLQQRQQLAEGCVPGAFGGPQGAEDPRRAADPTAPGSDSAVTLRGTSVERKREKGATVFA
NVAGDSFNTQNEPEDMMKKEKKSEALNIRGVLLHVMGDALGSVVVVITAIIFYVLPLKSEDPCNWQCYID
PSLTVLMVIIILSSAFPLIKETAAILLQMVPKGVNMEELMSKLSAVPGISSVHEVHIWELVSGKIIATLH
IKYPKDRGYQDASTKIREIFHHAGIHNVTIQFENVDLKEPLEQKDLLLLCNSPCISKGCAKQLCCPPGAL
PLAHVNGCAEHNGGPSLDTYGSDGLSRRDAREVAIEVSLDSCLSDHGQSLNKTQEDQCYVNRTHF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018713
ORF Size 1455 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018713.3
RefSeq Size 2869 bp
RefSeq ORF 1458 bp
Locus ID 55532
UniProt ID Q6XR72
Cytogenetics 1q41
Protein Families Transmembrane
MW 52.5 kDa
Summary This gene is highly expressed in the liver and is inducible by manganese. Its protein product appears to be critical in maintaining manganese levels, and has higher specificity for manganese than zinc. Loss of function mutations appear to result in a pleomorphic phenotype, including dystonia and adult-onset parkinsonism. Alternatively spliced transcript variants have been observed for this gene. provided by RefSeq, Mar 2012
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Documents
Resources
"NM_018713" in other vectors (6)
SKU Description Size Price
RC220219L1 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), Myc-DDK-tagged 10 ug
$757.00
RC220219L2 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), mGFP tagged 10 ug
$757.00
RC220219L3 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), Myc-DDK-tagged 10 ug
$757.00
RC220219L4 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), mGFP tagged 10 ug
$757.00
RG220219 SLC30A10 (tGFP-tagged) - Human solute carrier family 30, member 10 (SLC30A10) 10 ug
$489.00 $657.00
SC304629 SLC30A10 (untagged)-Human solute carrier family 30, member 10 (SLC30A10) 10 ug
$503.00

Plasmid Map for RC220219

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220219
Circular map for RC220219

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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