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SLC30A10 (NM_018713) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG220219
SLC30A10 (tGFP-tagged) - Human solute carrier family 30, member 10 (SLC30A10)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLC30A10
Synonyms HMDPC; HMNDYT1; ZnT-10; ZNT8; ZNT10; ZRC1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018713
ORF Size 1455 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018713.3
RefSeq Size 2869 bp
RefSeq ORF 1458 bp
Locus ID 55532
UniProt ID Q6XR72
Cytogenetics 1q41
Protein Families Transmembrane
Summary This gene is highly expressed in the liver and is inducible by manganese. Its protein product appears to be critical in maintaining manganese levels, and has higher specificity for manganese than zinc. Loss of function mutations appear to result in a pleomorphic phenotype, including dystonia and adult-onset parkinsonism. Alternatively spliced transcript variants have been observed for this gene. provided by RefSeq, Mar 2012
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"NM_018713" in other vectors (6)
SKU Description Size Price
RC220219 SLC30A10 (Myc-DDK-tagged)-Human solute carrier family 30, member 10 (SLC30A10) 10 ug
$457.00
RC220219L1 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), Myc-DDK-tagged 10 ug
$757.00
RC220219L2 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), mGFP tagged 10 ug
$757.00
RC220219L3 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), Myc-DDK-tagged 10 ug
$757.00
RC220219L4 Lenti ORF clone of Human solute carrier family 30, member 10 (SLC30A10), mGFP tagged 10 ug
$757.00
SC304629 SLC30A10 (untagged)-Human solute carrier family 30, member 10 (SLC30A10) 10 ug
$503.00

Plasmid Map for RG220219

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Cloning scheme for RG220219
Circular map for RG220219

file-alt Citations

Forced Overexpression and Knockout Analysis of SLC30A and SLC39A Family Genes Suggests Their Involvement in Establishing Resistance to Cisplatin in Human Cancer Cells
Kamynina, Rozenberg, Kushchenko et al
Int J Mol Sci (2024) 25 (22) DOI: 10.3390/ijms252212049
Cited by 7
AI Summary: OriGene’s SLC39A14, SLC39A8, SLC30A3, and SLC30A10 ORF clones were used to construct lentiviral vectors for gene overexpression studies, enabling the investigation of these genes’ roles in cisplatin resistance in human cancer cells.
Snippet: I, USA) in which the GFP was replaced by the puromycin resistance selection marker and the SLC39A8 (NM_022154.5), SLC30A3 (NM_003459.5), SLC30A10 (NM_018713.3), and SLC39A14 (NM_015359.6) ORF clones (Origene) were utilized for lentiviral vector construction. The genes SLC39A14, SLC39A8, SLC30A3, and SLC30A10 were amplified using high-fidelity DNA polymerase with primers designed to include EcoRI a
FigureFigureFigure
Showing 1-1 of 1 papers.
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