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SYCE1 (NM_130784) Human Tagged ORF Clone

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SKU
RC220187
SYCE1 (Myc-DDK-tagged)-Human synaptonemal complex central element protein 1 (SYCE1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SYCE1
Synonyms C10orf94; CT76; POF12; SPGF15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220187 representing NM_130784
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATGGTGCAAAAGCTGCAGAAAGTGGGAAGCCTAGAGCCCCGAGTTGAGGTCCTGATTAACCGGA
TTAATGAGGTCCAGCAAGCAAAAAAGAAAGCCAATAAAGACCTAGGAGAGGCCCGGACCATCTGTGAGGC
CCTGCAGAAGGAACTGGACTCGCTGCATGGAGAGAAAGTACACCTGAAGGAGATCTTGAGCAAAAAACAA
GAGACCCTGAGGATCCTCCGGCTGCATTGCCAGGAAAAGGAAAGTGAGGCACACAGGAAGCACACCATGT
TGCAGGAGTGCAAGGAGAGAATTTCTGCCCTGAACTTGCAGATTGAAGAAGAGAAGAACAAACAGAGACA
GCTGAGGTTGGCATTCGAGGAACAGCTGGAAGATCTGATGGGCCAGCACAAGGACCTCTGGGACTTCCAC
ATGCCAGAGCGGCTGGCAAAGGAGATTTGTGCCCTGGACAGCAGCAAGGAGCAGCTGCTCAAGGAAGAGA
AGCTGGTCAAGGCGACACTGGAAGACGTGAAGCATCAGCTGTGCTCCCTGTGTGGGGCTGAGGGCCCCTC
CACCCTTGATGAGGGACTCTTTCTCCGCAGCCAGGAGGCTGCAGCCACAGTGCAGCTGTTTCAGGAAGAG
CACAGGAAGGCTGAGGAGCTCCTAGCAGCTGCTGCCCAGCGCCACCAGCAGCTGCAGCAGAAGTGCCAAC
AACAGCAGCAGAAGCGGCAGAGGCTGAAGGAAGAGCTGGAAAAGCATGGAATGCAAGTCCCTGCCCAAGC
CCAGAGCACACAAGAGGAAGAGGCTGGCCCAGGAGATGTGGCTCCCAGACCAGGTAGACCAGTGACATGG
TGGAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220187 representing NM_130784
Red=Cloning site Green=Tags(s)

MEMVQKLQKVGSLEPRVEVLINRINEVQQAKKKANKDLGEARTICEALQKELDSLHGEKVHLKEILSKKQ
ETLRILRLHCQEKESEAHRKHTMLQECKERISALNLQIEEEKNKQRQLRLAFEEQLEDLMGQHKDLWDFH
MPERLAKEICALDSSKEQLLKEEKLVKATLEDVKHQLCSLCGAEGPSTLDEGLFLRSQEAAATVQLFQEE
HRKAEELLAAAAQRHQQLQQKCQQQQQKRQRLKEELEKHGMQVPAQAQSTQEEEAGPGDVAPRPGRPVTW
WS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_130784
ORF Size 846 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_130784.3
RefSeq Size 1400 bp
RefSeq ORF 849 bp
Locus ID 93426
UniProt ID Q8N0S2
Cytogenetics 10q26.3
MW 32.4 kDa
Summary This gene encodes a member of the synaptonemal complex, which links homologous chromosomes during prophase I of meiosis. The tripartite structure of the complex is highly conserved amongst metazoans. It consists of two lateral elements and a central region formed by transverse elements and a central element. The protein encoded by this gene localizes to the central element and is required for initiation and elongation of the synapsis. Allelic variants of this gene have been associated with premature ovarian failure and spermatogenic failure. Alternative splicing results in multiple transcript variants. provided by RefSeq, Nov 2016
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"NM_130784" in other vectors (4)
SKU Description Size Price
RC220187L3 Lenti ORF clone of Human synaptonemal complex central element protein 1 (SYCE1), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC220187L4 Lenti ORF clone of Human synaptonemal complex central element protein 1 (SYCE1), transcript variant 1, mGFP tagged 10 ug
$600.00
RG220187 SYCE1 (tGFP-tagged) - Human synaptonemal complex central element protein 1 (SYCE1), transcript variant 1 10 ug
$500.00
SC305905 SYCE1 (untagged)-Human synaptonemal complex central element protein 1 (SYCE1), transcript variant 1 10 ug
$330.00

Plasmid Map for RC220187

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220187
Circular map for RC220187

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