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BAG2 (NM_004282) Human Tagged ORF Clone

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SKU
RC220122
BAG2 (Myc-DDK-tagged)-Human BCL2-associated athanogene 2 (BAG2)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BAG2
Synonyms BAG-2; dJ417I1.2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC220122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGGCGAAGATCAACGCTAAAGCCAACGAGGGGCGCTTCTGCCGCTCCTCCTCCATGGCTGACC
GCTCCAGCCGCCTGCTGGAGAGCCTGGACCAGCTGGAGCTCAGGGTTGAAGCTTTGAGAGAAGCAGCAAC
TGCTGTTGAGCAAGAGAAAGAAATCCTTCTGGAAATGATCCACAGTATCCAAAATAGCCAGGACATGAGG
CAGATCAGTGACGGAGAAAGAGAAGAATTAAATCTGACTGCAAACCGTTTGATGGGAAGAACTCTCACCG
TTGAAGTGTCAGTAGAAACAATTAGAAACCCCCAGCAGCAAGAATCCCTAAAGCATGCCACAAGGATTAT
TGATGAGGTGGTCAATAAGTTTCTGGATGATTTGGGAAATGCCAAGAGTCATTTAATGTCGCTCTACAGT
GCATGTTCATCTGAGGTGCCACATGGGCCAGTTGATCAGAAGTTTCAATCCATAGTAATTGGCTGTGCTC
TTGAAGATCAGAAGAAAATTAAGAGAAGATTAGAGACTCTGCTTAGAAATATTGAAAACTCTGACAAGGC
CATCAAGCTATTAGAGCATTCTAAAGGAGCTGGTTCCAAAACTCTGCAACAAAATGCTGAAAGCAGATTC
AAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC220122 protein sequence
Red=Cloning site Green=Tags(s)

MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMR
QISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSHLMSLYS
ACSSEVPHGPVDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSKTLQQNAESRF
N

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004282
ORF Size 633 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004282.4
RefSeq Size 2068 bp
RefSeq ORF 636 bp
Locus ID 9532
UniProt ID O95816
Cytogenetics 6p12.1
Protein Families Druggable Genome
MW 23.8 kDa
Summary BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_004282" in other vectors (6)
SKU Description Size Price
RC220122L1 Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), Myc-DDK-tagged 10 ug
$600.00
RC220122L2 Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), mGFP tagged 10 ug
$600.00
RC220122L3 Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), Myc-DDK-tagged 10 ug
$600.00
RC220122L4 Lenti ORF clone of Human BCL2-associated athanogene 2 (BAG2), mGFP tagged 10 ug
$600.00
RG220122 BAG2 (tGFP-tagged) - Human BCL2-associated athanogene 2 (BAG2) 10 ug
$489.00 $500.00
SC303461 BAG2 (untagged)-Human BCL2-associated athanogene 2 (BAG2) 10 ug
$300.00

Plasmid Map for RC220122

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC220122
Circular map for RC220122

file-alt Citations

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