Wilms Tumor Protein (WT1) (NM_000378) Human Tagged ORF Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Wilms Tumor Protein |
| Synonyms | AWT1; GUD; NPHS4; WAGR; WIT-2; WT33 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000378 |
| ORF Size | 1491 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000378.6 |
| RefSeq Size | 2977 bp |
| RefSeq ORF | 1509 bp |
| Locus ID | 7490 |
| UniProt ID | P19544, Q6PI38 |
| Cytogenetics | 11p13 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 54.3 kDa |
| Summary | This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. provided by RefSeq, Mar 2015 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000378" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC220079L1 | Lenti ORF clone of Human Wilms tumor 1 (WT1), transcript variant A, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC220079L2 | Lenti ORF clone of Human Wilms tumor 1 (WT1), transcript variant A, mGFP tagged | 10 ug |
$757.00
|
|
| RC220079L3 | Lenti ORF clone of Human Wilms tumor 1 (WT1), transcript variant A, Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC220079L4 | Lenti ORF clone of Human Wilms tumor 1 (WT1), transcript variant A, mGFP tagged | 10 ug |
$757.00
|
|
| RG220079 | WT1 (tGFP-tagged) - Human Wilms tumor 1 (WT1), transcript variant A | 10 ug |
$489.00
$657.00
|
|
| SC300060 | WT1 (untagged)-Human Wilms tumor 1 (WT1), transcript variant A | 10 ug |
$457.00
|
|
Citations
TNFα-dependent modulation of WT1-MMP9 regulatory axis links developmental and inflammatory pathways in glaucoma
Ahmed, Sarma, Kabra et al
iScience (2026) 29 (9), 117176-117176 DOI: 10.1016/j.isci.2026.117176
Ahmed, Sarma, Kabra et al
iScience (2026) 29 (9), 117176-117176 DOI: 10.1016/j.isci.2026.117176
AI Summary: OriGene’s pCMV6-WT1 expression plasmid (Cat #RC220079) was used in this study to investigate the regulatory role of WT1 in human trabecular meshwork cells, enabling functional analysis of WT1’s effect on MMP9 expression relevant to glaucoma pathogenesis.
Snippet: pCMV6-WT1 expression plasmid OriGene Cat #RC220079 pGL3-Basic vector Promega 212936
The CUG-translated WT1, not AUG-WT1, is an oncogene
Lee, Jeon, Kim et al
Carcinogenesis (2017)
Lee, Jeon, Kim et al
Carcinogenesis (2017)
AI Summary: OriGene’s pCMV6-cugWT1 (Type B; (+) 17 aa/(-) KTS) plasmid was used in this study to express the CUG-translated WT1 protein, enabling the authors to investigate its oncogenic function in cancer cells.
Snippet: 6 Materials and Methods Reagents, cells and antibodies The pCMV6-cugWT1 (Type B;(+) 17 aa/ (-) KTS) was purchased from OriGene Technologies (Rockville, MD). The cugWT1 and augWT1 genes were re-cloned into the pCB6 and pcDNA4-Xpress/His vectors. ...;... 6 Materials and Methods Reagents, cells and antibodies The pCMV6-cugWT1 (Type B;(+) 17 aa/ (-) KTS) was purchased from OriGene Technologies (Rockville, MD). The cugWT1 and augWT1 genes were re-cloned into the pCB6 and pcDNA4-Xpress/His vectors.
Showing 1-2 of 2 papers.
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