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NVL (NM_206840) Human Tagged ORF Clone

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SKU
RC219929
NVL (Myc-DDK-tagged)-Human nuclear VCP-like (NVL), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $687.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NVL
Synonyms NVL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219929 representing NM_206840
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGACTACCCAGATCCACAGTCAGCAAATCACATGAACAGTTCCCTGCTGTCTTTATATCGGAAAG
GAAATCCTGATTCTGTTTCAAATACTCCTGAGATGGAGCAAAGAGAAACCACCTCTTCAACACCACGGAT
AAGTTCCAAAACAGGCTCCATTCCCTTGAAGACCCCTGCCAAAGATTCTGAAGGAGGATGGTTTATTGAC
AAAACCCCAAGTGTAAAGAAAGACAGTTTTTTCTTGGACCTGTCATGTGAGAAAAGTAATCCTAAGAAGC
CAATAACTGAGATACAGGATTCAAAAGATTCTTCTCTTTTGGAGAGTGATATGAAACGGAAAGGCAAGCT
AAAGAATAAAGGAAGCAAAAGGAAGAAAGAAGATCTTCAGGAAGTAGATGGAGAAATTGAAGCTGTCCTG
CAAAAGAAAGCTAAAGCCAGGGGGTTAGAATTCCAGATCTCCAACGTGAAGTTTGAAGATGTGGGAGGCA
ATGATATGACATTAAAAGAGGTCTGCAAGATGCTCATACACATGCGTCACCCGGAGGTGTACCACCACCT
GGGCGTCGTGCCCCCTCGTGGAGTTCTCCTTCATGGACCACCAGGCTGTGGGAAGACATTACTTGCACAT
GCAATTGCTGGGGAACTTGACCTGCCAATTTTGAAAGTGGCTGCTCCAGAGATTGTGTCTGGAGTATCCG
GAGAGTCTGAGCAGAAGCTGAGAGAACTATTTGAGCAAGCTGTGTCAAATGCACCATGTATCATTTTCAT
TGATGAAATTGATGCTATTACCCCCAAAAGAGAAGTGGCTTCAAAAGATATGGAACGAAGAATTGTAGCC
CAACTCCTAACCTGCATGGATGATCTGAATAATGTGGCTGCTACAGCCCGGGTCCTAGTTATTGGAGCTA
CTAATCGACCAGACTCGTTAGACCCTGCTTTGAGACGTGCGGGAAGGTTCGACCGAGAAATATGCCTAGG
TATCCCAGATGAAGCATCCAGGGAAAGAATACTTCAAACATTGTGCAGAAAACTGAGGCTTCCTCAAGCT
TTTGATTTCTGTCACTTAGCACACCTAACTCCAGGCTTTGTTGGTGCTGATCTCATGGCACTGTGCCGAG
AGGCAGCAATGTGTGCAGTCAATAGAGTCTTAATGAAGCTACAGGAACAGCAGAAGAAAAATCCTGAAAT
GGAAGATTTGCCATCTAAAGGAGTCCAGGAGGAAAGGCTGGGAACTGAGCCCACTTCTGAAACACAGGAT
GAATTACAAAGGCTGCTGGGGTTGCTAAGAGACCAAGATCCCCTCTCAGAGGAGCAGATGCAAGGACTGT
GCATTGAACTGAATGATTTCATTGTTGCTCTATCCTCAGTCCAACCCTCTGCCAAAAGGGAAGGCTTTGT
CACTGTCCCTAATGTGACATGGGCAGATATTGGTGCCCTGGAAGACATTAGAGAGGAGCTCACCATGGCA
ATATTGGCACCAGTACGCAACCCAGACCAGTTCAAAGCTCTTGGATTGGTGACTCCAGCTGGGGTCCTCC
TTGCTGGTCCTCCTGGCTGTGGGAAGACTCTGCTGGCGAAGGCTGTTGCAAATGAGTCCGGACTAAATTT
TATATCTGTCAAGGGCCCCGAATTACTAAACATGTATGTTGGTGAGAGTGAACGTGCTGTGCGACAAGTT
TTTCAACGAGCCAAGAACTCAGCACCCTGTGTGATATTCTTTGATGAAGTGGATGCTTTATGTCCTCGAA
GATCAGACCGAGAGACAGGGGCAAGTGTCCGAGTGGTGAATCAGCTACTTACAGAGATGGATGGTCTGGA
AGCACGCCAGCAGGTTTTTATTATGGCAGCCACTAACAGGCCAGATATAATTGACCCTGCAATCCTGCGC
CCGGGCCGCCTGGACAAAACACTGTTTGTGGGTTTACCGCCCCCTGCAGATCGCCTTGCCATCTTAAAAA
CTATCACAAAAAATGGTACCAAACCACCACTGGATGCAGATGTAAATTTGGAAGCAATTGCTGGTGACCT
TCGCTGTGATTGCTATACGGGCGCAGATCTCTCTGCTTTGGTACGAGAAGCTTCTATCTGTGCCCTGAGA
CAGGAAATGGCAAGACAGAAGAGTGGAAATGAAAAAGGTGAACTCAAGGTTAGTCATAAGCATTTTGAAG
AAGCTTTCAAGAAAGTAAGATCATCTATATCAAAAAAGGATCAAATCATGTATGAACGTTTGCAGGAGTC
CCTCAGCCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219929 representing NM_206840
Red=Cloning site Green=Tags(s)

MEDYPDPQSANHMNSSLLSLYRKGNPDSVSNTPEMEQRETTSSTPRISSKTGSIPLKTPAKDSEGGWFID
KTPSVKKDSFFLDLSCEKSNPKKPITEIQDSKDSSLLESDMKRKGKLKNKGSKRKKEDLQEVDGEIEAVL
QKKAKARGLEFQISNVKFEDVGGNDMTLKEVCKMLIHMRHPEVYHHLGVVPPRGVLLHGPPGCGKTLLAH
AIAGELDLPILKVAAPEIVSGVSGESEQKLRELFEQAVSNAPCIIFIDEIDAITPKREVASKDMERRIVA
QLLTCMDDLNNVAATARVLVIGATNRPDSLDPALRRAGRFDREICLGIPDEASRERILQTLCRKLRLPQA
FDFCHLAHLTPGFVGADLMALCREAAMCAVNRVLMKLQEQQKKNPEMEDLPSKGVQEERLGTEPTSETQD
ELQRLLGLLRDQDPLSEEQMQGLCIELNDFIVALSSVQPSAKREGFVTVPNVTWADIGALEDIREELTMA
ILAPVRNPDQFKALGLVTPAGVLLAGPPGCGKTLLAKAVANESGLNFISVKGPELLNMYVGESERAVRQV
FQRAKNSAPCVIFFDEVDALCPRRSDRETGASVRVVNQLLTEMDGLEARQQVFIMAATNRPDIIDPAILR
PGRLDKTLFVGLPPPADRLAILKTITKNGTKPPLDADVNLEAIAGDLRCDCYTGADLSALVREASICALR
QEMARQKSGNEKGELKVSHKHFEEAFKKVRSSISKKDQIMYERLQESLSR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_206840
ORF Size 2250 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_206840.2, NP_996671.1
RefSeq Size 2847 bp
RefSeq ORF 2253 bp
Locus ID 4931
UniProt ID O15381, B4DF43, O15381-2
Cytogenetics 1q42.11
MW 82.6 kDa
Summary This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin. provided by RefSeq, Aug 2011
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Documents
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"NM_206840" in other vectors (6)
SKU Description Size Price
RC219929L1 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, Myc-DDK-tagged 10 ug
$987.00
RC219929L2 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, mGFP tagged 10 ug
$987.00
RC219929L3 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, Myc-DDK-tagged 10 ug
$987.00
RC219929L4 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, mGFP tagged 10 ug
$987.00
RG219929 NVL (tGFP-tagged) - Human nuclear VCP-like (NVL), transcript variant 2 10 ug
$887.00
SC308282 NVL (untagged)-Human nuclear VCP-like (NVL), transcript variant 2 10 ug
$756.00

Plasmid Map for RC219929

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219929
Circular map for RC219929

file-alt Citations

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