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NVL (NM_206840) Human Untagged Clone

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SKU
SC308282
NVL (untagged)-Human nuclear VCP-like (NVL), transcript variant 2
  $756.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NVL
Synonyms NVL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC308282 representing NM_206840.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAAGACTACCCAGATCCACAGTCAGCAAATCACATGAACAGTTCCCTGCTGTCTTTATATCGGAAA
GGAAATCCTGATTCTGTTTCAAATACTCCTGAGATGGAGCAAAGAGAAACCACCTCTTCAACACCACGG
ATAAGTTCCAAAACAGGCTCCATTCCCTTGAAGACCCCTGCCAAAGATTCTGAAGGAGGATGGTTTATT
GACAAAACCCCAAGTGTAAAGAAAGACAGTTTTTTCTTGGACCTGTCATGTGAGAAAAGTAATCCTAAG
AAGCCAATAACTGAGATACAGGATTCAAAAGATTCTTCTCTTTTGGAGAGTGATATGAAACGGAAAGGC
AAGCTAAAGAATAAAGGAAGCAAAAGGAAGAAAGAAGATCTTCAGGAAGTAGATGGAGAAATTGAAGCT
GTCCTGCAAAAGAAAGCTAAAGCCAGGGGGTTAGAATTCCAGATCTCCAACGTGAAGTTTGAAGATGTG
GGAGGCAATGATATGACATTAAAAGAGGTCTGCAAGATGCTCATACACATGCGTCACCCGGAGGTGTAC
CACCACCTGGGCGTCGTGCCCCCTCGTGGAGTTCTCCTTCATGGACCACCAGGCTGTGGGAAGACATTA
CTTGCACATGCAATTGCTGGGGAACTTGACCTGCCAATTTTGAAAGTGGCTGCTCCAGAGATTGTGTCT
GGAGTATCCGGAGAGTCTGAGCAGAAGCTGAGAGAACTATTTGAGCAAGCTGTGTCAAATGCACCATGT
ATCATTTTCATTGATGAAATTGATGCTATTACCCCCAAAAGAGAAGTGGCTTCAAAAGATATGGAACGA
AGAATTGTAGCCCAACTCCTAACCTGCATGGATGATCTGAATAATGTGGCTGCTACAGCCCGGGTCCTA
GTTATTGGAGCTACTAATCGACCAGACTCGTTAGACCCTGCTTTGAGACGTGCGGGAAGGTTCGACCGA
GAAATATGCCTAGGTATCCCAGATGAAGCATCCAGGGAAAGAATACTTCAAACATTGTGCAGAAAACTG
AGGCTTCCTCAAGCTTTTGATTTCTGTCACTTAGCACACCTAACTCCAGGCTTTGTTGGTGCTGATCTC
ATGGCACTGTGCCGAGAGGCAGCAATGTGTGCAGTCAATAGAGTCTTAATGAAGCTACAGGAACAGCAG
AAGAAAAATCCTGAAATGGAAGATTTGCCATCTAAAGGAGTCCAGGAGGAAAGGCTGGGAACTGAGCCC
ACTTCTGAAACACAGGATGAATTACAAAGGCTGCTGGGGTTGCTAAGAGACCAAGATCCCCTCTCAGAG
GAGCAGATGCAAGGACTGTGCATTGAACTGAATGATTTCATTGTTGCTCTATCCTCAGTCCAACCCTCT
GCCAAAAGGGAAGGCTTTGTCACTGTCCCTAATGTGACATGGGCAGATATTGGTGCCCTGGAAGACATT
AGAGAGGAGCTCACCATGGCAATATTGGCACCAGTACGCAACCCAGACCAGTTCAAAGCTCTTGGATTG
GTGACTCCAGCTGGGGTCCTCCTTGCTGGTCCTCCTGGCTGTGGGAAGACTCTGCTGGCGAAGGCTGTT
GCAAATGAGTCCGGACTAAATTTTATATCTGTCAAGGGCCCCGAATTACTAAACATGTATGTTGGTGAG
AGTGAACGTGCTGTGCGACAAGTTTTTCAACGAGCCAAGAACTCAGCACCCTGTGTGATATTCTTTGAT
GAAGTGGATGCTTTATGTCCTCGAAGATCAGACCGAGAGACAGGGGCAAGTGTCCGAGTGGTGAATCAG
CTACTTACAGAGATGGATGGTCTGGAAGCACGCCAGCAGGTTTTTATTATGGCAGCCACTAACAGGCCA
GATATAATTGACCCTGCAATCCTGCGCCCGGGCCGCCTGGACAAAACACTGTTTGTGGGTTTACCGCCC
CCTGCAGATCGCCTTGCCATCTTAAAAACTATCACAAAAAATGGTACCAAACCACCACTGGATGCAGAT
GTAAATTTGGAAGCAATTGCTGGTGACCTTCGCTGTGATTGCTATACGGGCGCAGATCTCTCTGCTTTG
GTACGAGAAGCTTCTATCTGTGCCCTGAGACAGGAAATGGCAAGACAGAAGAGTGGAAATGAAAAAGGT
GAACTCAAGGTTAGTCATAAGCATTTTGAAGAAGCTTTCAAGAAAGTAAGATCATCTATATCAAAAAAG
GATCAAATCATGTATGAACGTTTGCAGGAGTCCCTCAGCCGGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_206840
Insert Size 2253 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_206840.2
RefSeq Size 2866 bp
RefSeq ORF 2253 bp
Locus ID 4931
UniProt ID O15381
Cytogenetics 1q42.11
MW 82.7 kDa
Summary This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) superfamily. Multiple transcript variants encoding different isoforms have been found for this gene. Two encoded proteins, described as major and minor isoforms, have been localized to distinct regions of the nucleus. The largest encoded protein (major isoform) has been localized to the nucleolus and shown to participate in ribosome biosynthesis (PMID: 15469983, 16782053), while the minor isoform has been localized to the nucleoplasmin. provided by RefSeq, Aug 2011
Transcript Variant: This variant (2) lacks an alternate exon and uses a downstream start codon compared to variant 1. The resulting protein (isoform 2, also known as NVL.1 or the minor isoform) is shorter and has a distinct N-terminus compared to isoform 1.
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Documents
Resources
"NM_206840" in other vectors (6)
SKU Description Size Price
RC219929 NVL (Myc-DDK-tagged)-Human nuclear VCP-like (NVL), transcript variant 2 10 ug
$687.00
RC219929L1 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, Myc-DDK-tagged 10 ug
$987.00
RC219929L2 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, mGFP tagged 10 ug
$987.00
RC219929L3 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, Myc-DDK-tagged 10 ug
$987.00
RC219929L4 Lenti ORF clone of Human nuclear VCP-like (NVL), transcript variant 2, mGFP tagged 10 ug
$987.00
RG219929 NVL (tGFP-tagged) - Human nuclear VCP-like (NVL), transcript variant 2 10 ug
$887.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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