NEU2 (NM_005383) Human Tagged ORF Clone

  • Product Brand Image
SKU
RC219858
NEU2 (Myc-DDK-tagged)-Human sialidase 2 (cytosolic sialidase) (NEU2)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NEU2
Synonyms SIAL2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219858 representing NM_005383
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCCCTTCCTGTCCTGCAGAAGGAGAGCGTGTTCCAGTCGGGAGCCCATGCCTACAGAATCCCTG
CCCTGCTCTACCTGCCTGGGCAGCAGTCCCTGCTGGCCTTCGCGGAACAGCGGGCAAGCAAGAAGGATGA
GCACGCAGAGCTGATTGTCCTGCGCAGAGGAGACTACGACGCACCCACCCACCAGGTTCAGTGGCAAGCT
CAGGAGGTGGTGGCCCAGGCCCGGCTGGATGGCCACCGGTCCATGAACCCATGCCCCTTGTATGACGCGC
AGACGGGGACCCTCTTCCTCTTCTTCATTGCCATCCCTGGGCAAGTCACGGAGCAACAGCAGCTGCAGAC
CAGGGCCAATGTGACGCGGCTGTGCCAAGTCACCAGCACTGACCACGGGAGGACCTGGAGCTCCCCCAGA
GACCTCACTGATGCGGCCATCGGCCCAGCCTACCGGGAGTGGTCCACCTTTGCAGTGGGCCCGGGGCATT
GTTTGCAGCTTAACGACAGGGCCCGGAGCCTGGTGGTGCCCGCCTACGCCTACCGGAAACTTCACCCCAT
CCAAAGGCCGATCCCCTCTGCCTTCTGCTTCCTCAGCCATGACCATGGGCGCACGTGGGCGCGAGGGCAC
TTTGTGGCCCAGGACACCCTGGAGTGCCAGGTGGCCGAAGTCGAGACTGGGGAGCAGAGGGTGGTGACCC
TCAACGCGAGAAGCCACCTCCGAGCCAGGGTCCAGGCCCAGAGCACCAATGACGGGCTTGATTTCCAGGA
GTCTCAGCTGGTGAAGAAGCTGGTGGAGCCGCCGCCCCAGGGCTGCCAGGGGAGCGTCATCAGCTTCCCC
AGCCCCCGCTCGGGGCCTGGCTCCCCAGCCCAGTGGCTGCTCTACACTCACCCCACACACTCCTGGCAGA
GGGCCGACCTGGGTGCCTACCTCAACCCGCGACCTCCAGCCCCTGAGGCCTGGTCAGAGCCGGTACTGCT
GGCCAAGGGCAGCTGTGCCTACTCAGACCTCCAGAGCATGGGCACCGGCCCTGATGGGTCCCCCTTGTTT
GGGTGTCTGTACGAAGCCAATGATTACGAGGAGATTGTCTTTCTCATGTTCACCCTGAAGCAAGCCTTCC
CAGCTGAGTACCTGCCTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC219858 representing NM_005383
Red=Cloning site Green=Tags(s)

MASLPVLQKESVFQSGAHAYRIPALLYLPGQQSLLAFAEQRASKKDEHAELIVLRRGDYDAPTHQVQWQA
QEVVAQARLDGHRSMNPCPLYDAQTGTLFLFFIAIPGQVTEQQQLQTRANVTRLCQVTSTDHGRTWSSPR
DLTDAAIGPAYREWSTFAVGPGHCLQLNDRARSLVVPAYAYRKLHPIQRPIPSAFCFLSHDHGRTWARGH
FVAQDTLECQVAEVETGEQRVVTLNARSHLRARVQAQSTNDGLDFQESQLVKKLVEPPPQGCQGSVISFP
SPRSGPGSPAQWLLYTHPTHSWQRADLGAYLNPRPPAPEAWSEPVLLAKGSCAYSDLQSMGTGPDGSPLF
GCLYEANDYEEIVFLMFTLKQAFPAEYLPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005383
ORF Size 1140 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005383.1, NP_005374.1
RefSeq Size 1143 bp
RefSeq ORF 1143 bp
Locus ID 4759
UniProt ID Q9Y3R4
Cytogenetics 2q37.1
Protein Pathways Other glycan degradation, Sphingolipid metabolism
MW 42.1 kDa
Summary This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. provided by RefSeq, Jul 2008
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_005383" in other vectors (6)
SKU Description Size Price
RC219858L1 Lenti ORF clone of Human sialidase 2 (cytosolic sialidase) (NEU2), Myc-DDK-tagged 10 ug
$757.00
RC219858L2 Lenti ORF clone of Human sialidase 2 (cytosolic sialidase) (NEU2), mGFP tagged 10 ug
$757.00
RC219858L3 Lenti ORF clone of Human sialidase 2 (cytosolic sialidase) (NEU2), Myc-DDK-tagged 10 ug
$757.00
RC219858L4 Lenti ORF clone of Human sialidase 2 (cytosolic sialidase) (NEU2), mGFP tagged 10 ug
$757.00
RG219858 NEU2 (tGFP-tagged) - Human sialidase 2 (cytosolic sialidase) (NEU2) 10 ug
$489.00 $657.00
SC303632 NEU2 (untagged)-Human sialidase 2 (cytosolic sialidase) (NEU2) 10 ug
$457.00

Plasmid Map for RC219858

Download sequence for

Cloning scheme for RC219858

Circular map for RC219858

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products