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HES7 (NM_032580) Human Tagged ORF Clone

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SKU
RC219701
HES7 (Myc-DDK-tagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HES7
Synonyms bHLHb37; SCDO4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219701 representing NM_032580
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCACCCGGGATCGAGCTGAGAATAGGGACGGCCCCAAGATGCTCAAGCCGCTTGTGGAGAAGCGGC
GCCGGGACCGCATCAACCGCAGCCTGGAAGAGCTGAGGCTGCTGCTGCTGGAGCGGACCCGGGACCAGAA
CCTCCGGAACCCGAAGCTGGAGAAAGCGGAGATATTGGAGTTCGCCGTGGGCTACTTGAGGGAGCGAAGC
CGGGTGGAGCCCCCGGGGGTTCCCCGGTCCCCAGTCCAGGACGCCGAGGCGCTCGCCAGCTGCTACTTGT
CCGGTTTCCGCGAGTGCCTGCTTCGCTTGGCGGCCTTCGCGCACGACGCCAGCCCGGCCGCCCGCGCCCA
GCTCTTCTCCGCGCTGCACGGCTATCTGCGCCCCAAACCGCCCCGGCCCAAGCCGGTAGATCCGAGGCCT
CCAGCGCCGCGCCCATCCCTGGACCCCGCCGCACCGGCCCTTGGCCCTGCGCTGCACCAGCGCCCCCCAG
TGCACCAGGGCCACCCTAGCCCGCGCTGCGCATGGTCCCCATCCCTCTGCTCCCCGCGCGCCGGGGATTC
TGGCGCGCCGGCGCCCCTCACCGGACTGCTGCCGCCGCCACCGCCGCCTCACAGACAAGACGGGGCGCCC
AAGGCCCCGCTGCCCCCGCCGCCCGCTTTCTGGAGACCTTGGCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219701 representing NM_032580
Red=Cloning site Green=Tags(s)

MVTRDRAENRDGPKMLKPLVEKRRRDRINRSLEELRLLLLERTRDQNLRNPKLEKAEILEFAVGYLRERS
RVEPPGVPRSPVQDAEALASCYLSGFRECLLRLAAFAHDASPAARAQLFSALHGYLRPKPPRPKPVDPRP
PAPRPSLDPAAPALGPALHQRPPVHQGHPSPRCAWSPSLCSPRAGDSGAPAPLTGLLPPPPPPHRQDGAP
KAPLPPPPAFWRPWP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_032580
ORF Size 675 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_032580.4
RefSeq Size 678 bp
RefSeq ORF 678 bp
Locus ID 84667
UniProt ID F8VPC9, Q9BYE0
Cytogenetics 17p13.1
MW 24.7 kDa
Summary This gene encodes a member of the hairy and enhancer of split family of bHLH transcription factors. The mouse ortholog of this gene is regulated by Notch signaling. The protein functions as a transcriptional repressor, and is implicated in correct patterning of the axial skeleton. A mutation in this gene has been shown to result in spondylocostal dysostosis. Multiple transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Sep 2009
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"NM_032580" in other vectors (6)
SKU Description Size Price
RC219701L1 Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC219701L2 Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, mGFP tagged 10 ug
$600.00
RC219701L3 Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC219701L4 Lenti ORF clone of Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2, mGFP tagged 10 ug
$600.00
RG219701 HES7 (tGFP-tagged) - Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2 10 ug
$489.00 $500.00
SC305482 HES7 (untagged)-Human hairy and enhancer of split 7 (Drosophila) (HES7), transcript variant 2 10 ug
$300.00

Plasmid Map for RC219701

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219701
Circular map for RC219701

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