VPS37C (NM_017966) Human Tagged ORF Clone

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SKU
RC219699
VPS37C (Myc-DDK-tagged)-Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol VPS37C
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219699 representing NM_017966
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACGCTGAAGGATAAGACCCTGCAGGAGCTGGAGGAGTTGCAGAATGACTCGGAGGCGATTGACC
AGCTGGCCCTGGAGTCCCCTGAGGTCCAGGACCTACAGCTGGAACGGGAGATGGCACTGGCCACCAACCG
GAGCCTGGCAGAGCGGAACTTGGAGTTCCAGGGTCCCCTGGAGATCAGCCGCTCAAACCTCTCGGATAGA
TACCAGGAGCTCCGGAAGCTCGTGGAGCGGTGCCAGGAGCAGAAGGCAAAGCTGGAGAAATTTTCTTCAG
CACTGCAGCCAGGGACCTTGTTAGACCTTCTGCAGGTGGAAGGCATGAAGATCGAAGAAGAGTCCGAGGC
CATGGCTGAGAAGTTCCTGGAGGGCGAGGTGCCCCTGGAAACGTTCCTGGAGAATTTTTCCTCCATGAGG
ATGCTGTCCCACCTGCGCCGGGTTCGCGTGGAAAAGCTCCAGGAAGTGGTGAGGAAGCCCAGGGCTTCCC
AGGAGCTGGCCGGCGATGCCCCTCCACCCCGTCCACCACCCCCGGTGCGCCCAGTCCCCCAGGGAACACC
CCCTGTGGTTGAAGAGCAGCCGCAGCCACCATTAGCCATGCCTCCCTACCCTTTGCCCTACAGCCCATCC
CCCAGCCTGCCTGTGGGCCCCACTGCCCATGGAGCCCTGCCACCGGCCCCTTTCCCAGTAGTGTCCCAGC
CCTCCTTCTACAGCGGGCCTCTGGGCCCCACTTACCCGGCAGCCCAGCTTGGACCCAGGGGTGCTGCGGG
TTACTCCTGGTCCCCACAGAGGAGCATGCCACCCCGGCCGGGCTATCCTGGGACCCCAATGGGTGCCTCT
GGGCCTGGGTACCCCTTGCGGGGAGGCAGGGCCCCCAGTCCTGGTTATCCTCAACAGTCCCCATACCCCG
CAACAGGAGGAAAACCTCCCTACCCAATACAGCCTCAGCTCCCCAGCTTTCCAGGCCAGCCCCAGCCCTC
AGTGCCCCTGCAGCCCCCTTATCCCCCCGGGCCCGCCCCTCCCTATGGGTTCCCACCACCGCCGGGGCCT
GCCTGGCCTGGGTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC219699 representing NM_017966
Red=Cloning site Green=Tags(s)

METLKDKTLQELEELQNDSEAIDQLALESPEVQDLQLEREMALATNRSLAERNLEFQGPLEISRSNLSDR
YQELRKLVERCQEQKAKLEKFSSALQPGTLLDLLQVEGMKIEEESEAMAEKFLEGEVPLETFLENFSSMR
MLSHLRRVRVEKLQEVVRKPRASQELAGDAPPPRPPPPVRPVPQGTPPVVEEQPQPPLAMPPYPLPYSPS
PSLPVGPTAHGALPPAPFPVVSQPSFYSGPLGPTYPAAQLGPRGAAGYSWSPQRSMPPRPGYPGTPMGAS
GPGYPLRGGRAPSPGYPQQSPYPATGGKPPYPIQPQLPSFPGQPQPSVPLQPPYPPGPAPPYGFPPPPGP
AWPGY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017966
ORF Size 1065 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017966.5
RefSeq Size 2711 bp
RefSeq ORF 1068 bp
Locus ID 55048
UniProt ID A5D8V6
Cytogenetics 11q12.2
Protein Pathways Endocytosis
MW 38.5 kDa
Summary VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 PubMed 15509564).supplied by OMIM, Mar 2008
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Documents
Resources
"NM_017966" in other vectors (5)
SKU Description Size Price
RC219699L3 Lenti ORF clone of Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C), Myc-DDK-tagged 10 ug
$757.00
RC219699L4 Lenti ORF clone of Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C), mGFP tagged 10 ug
$757.00
RG219699 VPS37C (tGFP-tagged) - Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C) 10 ug
$657.00
SC113816 VPS37C (untagged)-Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C) 10 ug
$150.00
SC317562 VPS37C (untagged)-Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C) 10 ug
$503.00

Plasmid Map for RC219699

Download sequence for

Cloning scheme for RC219699

Circular map for RC219699

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