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COX8C (NM_182971) Human Tagged ORF Clone

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SKU
RC219625
COX8C (Myc-DDK-tagged)-Human cytochrome c oxidase subunit VIIIC (COX8C), nuclear gene encoding mitochondrial protein
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COX8C
Synonyms COX8-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219625 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCTCTCCTGCGTGGGCGCTGTCCTGCCCGCCGCCACTACCGCCGCTTGGCCCTGCTCGGCCTGCAGC
CCGCTCCCCGCTTCGCCCACTCGGGGCCCCCGCGCCAGCGGCCCCTGTCTGCCGCGGAAATGGCTGTTGG
ACTTGTGGTGTTTTTTACGACCTTCTTAACACCAGCTGCATATGTGCTAGGCAACCTGAAGCAGTTCAGA
AGGAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219625 protein sequence
Red=Cloning site Green=Tags(s)

MPLLRGRCPARRHYRRLALLGLQPAPRFAHSGPPRQRPLSAAEMAVGLVVFFTTFLTPAAYVLGNLKQFR
RN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_182971
ORF Size 216 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_182971.3
RefSeq Size 531 bp
RefSeq ORF 219 bp
Locus ID 341947
UniProt ID Q7Z4L0
Cytogenetics 14q32.12
Protein Families Transmembrane
Protein Pathways Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
MW 8.1 kDa
Summary This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.UniProtKB/Swiss-Prot Function
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"NM_182971" in other vectors (4)
SKU Description Size Price
RC219625L3 Lenti ORF clone of Human cytochrome c oxidase subunit VIIIC (COX8C), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$450.00
RC219625L4 Lenti ORF clone of Human cytochrome c oxidase subunit VIIIC (COX8C), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$450.00
RG219625 COX8C (tGFP-tagged) - Human cytochrome c oxidase subunit VIIIC (COX8C), nuclear gene encoding mitochondrial protein 10 ug
$489.00
SC307546 COX8C (untagged)-Human cytochrome c oxidase subunit VIIIC (COX8C), nuclear gene encoding mitochondrial protein 10 ug
$165.00

Plasmid Map for RC219625

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219625
Circular map for RC219625

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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