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DACH2 (NM_053281) Human Tagged ORF Clone

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SKU
RC219595
DACH2 (Myc-DDK-tagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $558.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DACH2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219595 representing NM_053281
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCTCCGCATCTCCAGTGATCTCTGCAACTTCCAGCGGCGCCGGCGTCCCGGGGGGCTTATTCC
GGGCCGAACCCCTGTACTCGACTCCCAGAGAGCCCCCTCGTCTTACTCCTAATATGATCAATAGTTTCGT
GGTTAATAACCACAGCAACAGTGCCGGAGGCGGCGGCAGGGGCAACACCAACACCAACGAGTGCCGCATG
GTCGACATGCACGGGATGAAGGTGGCTTCGTTCCTGATGGACGGCCAGGAACTGATCTGCCTGCCGCAAG
TCTTTGATCTTTTTCTCAAGCACCTGGTGGGAGGCTTGCACACTGTGTACACCAAGCTGAAGAGACTGGA
TATATCCCCCGTGGTGTGTACTGTTGAGCAGGTCCGGATCCTCCGCGGGCTGGGGGCCATCCAGCCCGGG
GTAAACCGCTGCAAACTCATCACCAGGAAAGACTTCGAAACTTTGTTCACCGATTGCACCAATGCCAGAA
GGAAGAGGCAAATGACAAGAAAACAAGCTGTTAACAGTTCAAGACCCGGCAGGCCCCCTAAGCGTTCTTT
GGGAGTGTTGCAGGAAAATGCCCGCCTTCTGACCCATGCAGTCCCAGGCCTCTTATCGCCAGGACTTATC
ACTCCGACAGGTATAACAGCTGCAGCGATGGCTGAGGCGATGAAACTTCAGAAGATGAAGCTTATGGCTA
TGAACACTCTTCAGGGAAATGGAAGCCAAAATGGGACCGAATCAGAGCCTGATGATCTTAATTCTAACAC
AGGTGGAAGTGAATCCTCCTGGGATAAAGATAAGATGCAGTCTCCATTTGCTGCACCTGGACCCCAACAT
GGAATTGCTCATGCAGCCCTAGCTGGCCAGCCAGGCATTGGGGGTGCTCCAACCCTCAATCCACTGCAGC
AGAACCACCTGCTAACCAATAGACTGGATCTGCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAG
CTTACCTCCTGCATCAGTTGCCATGGCAATGAATCAGATGAACCATCTCAATACTATTGCCAACATGGCT
GCTGCAGCACAGATTCACAGTCCACTCTCCAGAGCTGGTACCTCTGTTATAAAGGAGCGGATCCCAGAGA
GTCCTTCTCCTGCTCCTTCTCTAGAAGAGAATCATCGTCCTGGGAGCCAGACCTCTTCCCACACCAGCAG
CAGTGTGTCCAGCTCTCCCTCTCAGATGGATCATCATTTGGAAAGAATGGAAGAGGTACCAGTTCAAATT
CCAATAATGAAGTCACCCTTGGACAAGATACAGCTGACTCCTGGGCAGGCATTGCCCGCTGGATTCCCTG
GACCATTCATTTTTGCTGATAGTCTGTCCTCCGTGGAGACTCTGTTGACCAACATTCAGGGTCTGCTGAA
AGTTGCTTTGGATAATGCTCGCATCCAGGAGAAGCAGATTCAACAAGAAAAGAAGGAGCTGCGACTGGAG
CTCTATAGAGAGAGAGAAATTAGAGAAAACCTTGAAAGACAACTTGCAGTTGAGCTTCAAAGCAGAACTA
CTATGCAAAAGCGCCTGAAGAAGGAGAAAAAAACCAAGAGAAAATTGCAGGAAGCCTTGGAATTTGAATC
AAAGCGCCGGGAGCAAGTGGAGCAGGCACTTAAGCAAGCCACCACTAGTGACAGTGGCCTGAGGATGTTA
AAAGATACTGGAATTCCAGATATTGAAATAGAAAACAATGGGACTCCTCATGATAGTGCTGCTATGCAAG
GAGGTAACTATTACTGTTTAGAAATGGCACAACAGTTGTATTCAGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219595 representing NM_053281
Red=Cloning site Green=Tags(s)

MAVSASPVISATSSGAGVPGGLFRAEPLYSTPREPPRLTPNMINSFVVNNHSNSAGGGGRGNTNTNECRM
VDMHGMKVASFLMDGQELICLPQVFDLFLKHLVGGLHTVYTKLKRLDISPVVCTVEQVRILRGLGAIQPG
VNRCKLITRKDFETLFTDCTNARRKRQMTRKQAVNSSRPGRPPKRSLGVLQENARLLTHAVPGLLSPGLI
TPTGITAAAMAEAMKLQKMKLMAMNTLQGNGSQNGTESEPDDLNSNTGGSESSWDKDKMQSPFAAPGPQH
GIAHAALAGQPGIGGAPTLNPLQQNHLLTNRLDLPFMMMPHPLLPVSLPPASVAMAMNQMNHLNTIANMA
AAAQIHSPLSRAGTSVIKERIPESPSPAPSLEENHRPGSQTSSHTSSSVSSSPSQMDHHLERMEEVPVQI
PIMKSPLDKIQLTPGQALPAGFPGPFIFADSLSSVETLLTNIQGLLKVALDNARIQEKQIQQEKKELRLE
LYREREIRENLERQLAVELQSRTTMQKRLKKEKKTKRKLQEALEFESKRREQVEQALKQATTSDSGLRML
KDTGIPDIEIENNGTPHDSAAMQGGNYYCLEMAQQLYSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053281
ORF Size 1797 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053281.3
RefSeq Size 2232 bp
RefSeq ORF 1800 bp
Locus ID 117154
UniProt ID Q96NX9, A8K3I1
Cytogenetics Xq21.2
Domains Ski_Sno
Protein Families Transcription Factors
MW 65.1 kDa
Summary This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2008
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"NM_053281" in other vectors (4)
SKU Description Size Price
RC219595L3 Lenti ORF clone of Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 1, Myc-DDK-tagged 10 ug
$858.00
RC219595L4 Lenti ORF clone of Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 1, mGFP tagged 10 ug
$858.00
RG219595 DACH2 (tGFP-tagged) - Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 1 10 ug
$758.00
SC310228 DACH2 (untagged)-Human dachshund homolog 2 (Drosophila) (DACH2), transcript variant 1 10 ug
$558.00

Plasmid Map for RC219595

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219595
Circular map for RC219595

file-alt Citations

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