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TMEM185B (NR_000034) Human Tagged ORF Clone

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SKU
RC219480
TMEM185B (Myc-DDK-tagged)-Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMEM185B
Synonyms FAM11B; FLJ20979
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219480 representing NR_000034
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCCAGGGGCCTGTTCCAGGACTTCAACCCCAGTAAGTTTCTCATCTACACCTGCCTGCTGCTCT
TCTCGGTGCTGCTGCCCCTCCGCCTGGACGGCATCATCCAATGGAGCTACTGGGCCGTCTTTGCCCCCAT
ATGGCTGTGGAAGCTTCTAGTCGTCGCAGGCGCCTCCGTGGGCGCGGGCGTTTGGGCCCGCAACCCTCGC
TACCGCACCGAGGGAGAGGCCTGTGTGGAGTTCAAAGCCATGCTGATCGCTGTGGGCATCCACCTGCTGC
TGCTCATGTTCGAAGTCCTGGTCTGCGACAGGGTGGAGAGGGGCACCCACTTCTGGCTGCTGGTCTTCAT
GCCTCTCTTCTTCGTGTCCCCCGTGTCCGTGGCTGCCTGCGTCTGGGGCTTTCGACACGATAGGTCGCTG
GAGCTGGAGATCCTGTGCTCGGTCAACATCCTGCAGTTCATCTTCATCGCCCTAAAGCTGGACAGGATTA
TTCACTGGCCGTGGCTGGTGGTGTTTGTGCCCCTGTGGATCCTCATGTCGTTCCTTTGCCTGGTCGTCCT
CTATTACATCGTCTGGTCCCTCCTGTTCCTGCGGTCCCTGGATGTGGTTGCCGAGCAGCGGAGAACACAC
GTGACCATGGCTATCAGTTGGATAACGATTGTCGTGCCTCTGCTCACTTTTGAGGTCCTGCTGGTTCACA
GATTGGATGGCCACAATACATTCTCCTACGTCTCCATATTTGTCCCCCTTTGGCTTTCCTTACTAACTTT
AATGGCCACAACATTTAGGCGAAAGGGGGGCAATCATTGGTGGTTTGGCATTCGCAGAGACTTCTGTCAG
TTTCTGCTTGAAATTTTCCCATTTTTAAGAGAATATGGGAACATTTCATATGATCTCCATCACGAAGATA
GTGAAGATGCTGAAGAAACATCAGTTCCAGAAGCTCCGAAAATTGCTCCAATATTTGGAAAGAAGGCCAG
AGTAGTTATAACCCAGAGCCCTGGGAAATACGTTCCCCCCCCTCCCAAGTTAAATATTGATATGCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219480 representing NR_000034
Red=Cloning site Green=Tags(s)

MNPRGLFQDFNPSKFLIYTCLLLFSVLLPLRLDGIIQWSYWAVFAPIWLWKLLVVAGASVGAGVWARNPR
YRTEGEACVEFKAMLIAVGIHLLLLMFEVLVCDRVERGTHFWLLVFMPLFFVSPVSVAACVWGFRHDRSL
ELEILCSVNILQFIFIALKLDRIIHWPWLVVFVPLWILMSFLCLVVLYYIVWSLLFLRSLDVVAEQRRTH
VTMAISWITIVVPLLTFEVLLVHRLDGHNTFSYVSIFVPLWLSLLTLMATTFRRKGGNHWWFGIRRDFCQ
FLLEIFPFLREYGNISYDLHHEDSEDAEETSVPEAPKIAPIFGKKARVVITQSPGKYVPPPPKLNIDMP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NR_000034
ORF Size 1047 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NR_000034.1
RefSeq Size 2131 bp
RefSeq ORF 1049 bp
Locus ID 79134
Cytogenetics 2q14.2
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"NR_000034" in other vectors (4)
SKU Description Size Price
RC219480L3 Lenti ORF clone of Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA, Myc-DDK-tagged 10 ug
$757.00
RC219480L4 Lenti ORF clone of Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA, mGFP tagged 10 ug
$757.00
RG219480 TMEM185B (tGFP-tagged) - Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA 10 ug
$657.00
SC126776 TMEM185B (untagged)-Human transmembrane protein 185B (pseudogene) (TMEM185B), non-coding RNA 10 ug
$300.00

Plasmid Map for RC219480

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219480
Circular map for RC219480

file-alt Citations

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