AP3D1 (NM_003938) Human Tagged ORF Clone

SKU RC219366

AP3D1 (Myc-DDK-tagged)-Human adaptor-related protein complex 3, delta 1 subunit (AP3D1), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: AP3D1
• Synonyms: ADTD; hBLVR; HPS10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219366 representing NM_003938; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCCTCAAGATGGTGAAGGGCAGCATCGACCGCATGTTCGACAAGAATCTGCAGGACTTGGTCCGCG; GCATCCGTAACCACAAGGAGGACGAGGCAAAATACATATCTCAGTGCATTGATGAGATCAAGCAGGAGCT; GAAGCAGGACAACATAGCGGTGAAGGCGAACGCGGTCTGCAAGCTGACGTATTTACAGATGTTGGGATAC; GACATCAGCTGGGCCGCCTTCAACATCATAGAAGTGATGAGTGCCTCCAAGTTCACCTTCAAGCGAATTG; GCTACCTCGCTGCTTCCCAGAGCTTTCACGAAGGCACCGACGTCATCATGCTGACCACCAATCAGATCCG; TAAGGACTTGAGCAGCCCCAGCCAGTACGACACAGGTGTTGCACTGACGGGTCTGTCCTGCTTCGTCACC; CCAGACCTTGCCAGAGACCTGGCAAATGACATCATGACACTGATGTCACACACCAAGCCCTACATCAGGA; AGAAGGCTGTGCTGATCATGTACAAGGTGTTCCTGAAGTACCCCGAGTCGCTGCGCCCTGCCTTTCCCCG; GCTGAAGGAGAAGCTGGAGGACCCCGACCCCGGGGTTCAGTCGGCTGCCGTCAATGTCATCTGCGAGCTG; GCCAGACGCAACCCTAAGAACTACCTGTCCCTGGCCCCGCTCTTTTTCAAGCTGATGACGTCCTCCACCA; ACAACTGGGTCCTCATCAAGATCATCAAGCTGTTCGGTGCTCTTACTCCTTTGGAACCGCGGCTGGGCAA; GAAGCTGATCGAGCCCCTCACCAATCTCATCCACAGCACGTCTGCCATGTCTCTCCTCTATGAATGTGTG; AACACCGTGATTGCAGTGCTCATCTCGCTGTCCTCCGGCATGCCCAACCACAGCGCCAGCATCCAGCTTT; GTGTTCAGAAATTAAGGATATTGATCGAGGACTCCGATCAGAACTTGAAGTACCTGGGGCTGCTGGCAAT; GTCCAAGATCCTGAAGACCCACCCCAAGTCCGTGCAGTCCCACAAGGACCTCATCCTGCAGTGCCTGGAC; GACAAGGACGAGTCCATCCGGCTGCGGGCCCTGGACCTGCTCTATGGGATGGTGTCCAAGAAGAACCTGA; TGGAGATCGTGAAGAAGCTGATGACCCACGTAGACAAGGCAGAGGGTACCACCTACCGTGACGAGCTGCT; CACCAAGATCATTGACATCTGCAGCCAGTCCAACTACCAGTACATCACCAACTTCGAGTGGTACATCAGC; ATCCTGGTGGAGCTGACCCGGCTGGAGGGCACACGGCACGGCCACCTCATCGCCGCCCAAATGCTGGACG; TGGCCATCCGCGTGAAGGCCATCCGCAAGTTCGCCGTGTCCCAGATGTCTGCGCTGCTTGACAGTGCACA; CCTGCTGGCCAGCAGCACCCAGCGGAACGGGATCTGTGAGGTGCTGTACGCTGCCGCCTGGATCTGCGGG; GAGTTCTCAGAGCATCTGCAGGAACCACACCACACTTTGGAGGCCATGCTGCGGCCCAGAGTCACCACGC; TGCCAGGCCACATCCAGGCCGTGTATGTGCAGAACGTGGTCAAGCTCTACGCCTCCATCCTGCAGCAGAA; GGAGCAGGCCGGGGAGGCAGAGGGCGCTCAGGCCGTCACCCAGCTCATGGTGGACCGGCTGCCCCAGTTT; GTGCAGAGCGCAGACCTGGAGGTGCAGGAGCGGGCGTCCTGCATCCTGCAGCTGGTCAAGCACATCCAGA; AGCTTCAGGCCAAGGACGTGCCTGTGGCAGAGGAGGTCAGCGCTCTCTTTGCTGGGGAGCTGAACCCAGT; GGCCCCCAAGGCCCAGAAGAAGGTTCCAGTCCCCGAAGGCCTGGACCTGGACGCCTGGATCAATGAGCCA; CTCTCGGACAGCGAGTCAGAGGACGAGAGGCCCAGGGCCGTCTTCCACGAGGAGGAGCAGCGGCGTCCCA; AGCACCGGCCGTCGGAGGCGGACGAGGAAGAGCTGGCTCGGCGCCGAGAGGCCCGGAAGCAGGAGCAGGC; CAACAACCCCTTCTACATCAAGAGCTCGCCATCGCCACAGAAGCGGTACCAGGACACCCCGGGCGTGGAG; CACATTCCCGTGGTGCAGATTGACCTCTCCGTCCCCTTGAAGGTTCCAGGGCTGCCTATGTCAGATCAGT; ATGTGAAGCTGGAGGAGGAGCGGCGGCACCGGCAGAAGCTGGAGAAGGACAAGAGGAGGAAAAAGAGGAA; GGAGAAGGAGAAGAAGGGCAAGCGCCGCCACAGCTCGCTGCCCACGGAGAGCGACGAGGACATCGCCCCT; GCCCAGCAGGTGGACATCGTCACAGAGGAGATGCCTGAGAATGCTCTGCCCAGCGACGAGGATGACAAAG; ACCCCAACGACCCCTACAGGGCTCTGGATATTGACCTGGATAAGCCCTTAGCCGACAGCGAGAAACTGCC; TATTCAGAAACACAGAAACACCGAGACCTCAAAATCCCCTGAGAAGGACGTTCCCATGGTAGAAAAGAAG; AGCAAGAAACCCAAGAAGAAAGAGAAAAAACACAAAGAGAAAGAGAGAGACAAGGAGAAGAAGAAGGAGA; AGGAGAAGAAGAAATCTCCCAAGCCTAAGAAGAAGAAGCACAGGAAGGAGAAGGAGGAGCGGACCAAAGG; CAAGAAGAAGTCCAAGAAGCAGCCTCCAGGCAGCGAGGAGGCAGCGGGGGAGCCGGTGCAGAATGGCGCG; CCAGAGGAGGAGCAGCTCCCGCCTGAGTCCAGCTACTCCCTCCTCGCTGAAAATTCCTATGTTAAAATGA; CCTGTGACATCCGGGGCAGTCTGCAGGAGGACAGCCAGGTCACTGTGGCCATCGTGCTGGAGAACAGGAG; CAGCAGCATCCTCAAGGGCATGGAGCTCAGCGTGCTGGACTCACTCAATGCCAGGATGGCCCGGCCGCAG; GGCTCCTCCGTCCACGATGGCGTCCCCGTGCCTTTCCAGCTGCCCCCAGGCGTCTCCAACGAAGCCCAGT; ATGTGTTCACCATCCAGAGCATCGTCATGGCGCAGAAGCTCAAGGGGACCCTGTCCTTCATTGCCAAGAA; TGACGAGGGTGCGACCCACGAGAAGCTGGACTTCAGGCTGCACTTCAGCTGCAGCTCCTACTTGATCACC; ACTCCCTGCTACAGTGACGCCTTTGCTAAGTTGCTGGAGTCTGGGGACTTGAGCATGAGCTCAATCAAAG; TCGATGGCATTCGGATGTCCTTCCAGAATCTTCTGGCGAAGATCTGTTTTCACCACCATTTTTCCGTTGT; GGAGCGAGTGGACTCCTGCGCCTCCATGTACAGCCGCTCCATCCAGGGCCACCATGTCTGCCTCCTGGTG; AAAAAGGGTGAGAACTCTGTCTCAGTCGACGGGAAGTGCAGTGACTCCACGCTACTGAGCAACTTGTTAG; AAGAGATGAAGGCGACGCTGGCCAAGTGT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219366 representing NM_003938; Red=Cloning site Green=Tags(s); ; MALKMVKGSIDRMFDKNLQDLVRGIRNHKEDEAKYISQCIDEIKQELKQDNIAVKANAVCKLTYLQMLGY; DISWAAFNIIEVMSASKFTFKRIGYLAASQSFHEGTDVIMLTTNQIRKDLSSPSQYDTGVALTGLSCFVT; PDLARDLANDIMTLMSHTKPYIRKKAVLIMYKVFLKYPESLRPAFPRLKEKLEDPDPGVQSAAVNVICEL; ARRNPKNYLSLAPLFFKLMTSSTNNWVLIKIIKLFGALTPLEPRLGKKLIEPLTNLIHSTSAMSLLYECV; NTVIAVLISLSSGMPNHSASIQLCVQKLRILIEDSDQNLKYLGLLAMSKILKTHPKSVQSHKDLILQCLD; DKDESIRLRALDLLYGMVSKKNLMEIVKKLMTHVDKAEGTTYRDELLTKIIDICSQSNYQYITNFEWYIS; ILVELTRLEGTRHGHLIAAQMLDVAIRVKAIRKFAVSQMSALLDSAHLLASSTQRNGICEVLYAAAWICG; EFSEHLQEPHHTLEAMLRPRVTTLPGHIQAVYVQNVVKLYASILQQKEQAGEAEGAQAVTQLMVDRLPQF; VQSADLEVQERASCILQLVKHIQKLQAKDVPVAEEVSALFAGELNPVAPKAQKKVPVPEGLDLDAWINEP; LSDSESEDERPRAVFHEEEQRRPKHRPSEADEEELARRREARKQEQANNPFYIKSSPSPQKRYQDTPGVE; HIPVVQIDLSVPLKVPGLPMSDQYVKLEEERRHRQKLEKDKRRKKRKEKEKKGKRRHSSLPTESDEDIAP; AQQVDIVTEEMPENALPSDEDDKDPNDPYRALDIDLDKPLADSEKLPIQKHRNTETSKSPEKDVPMVEKK; SKKPKKKEKKHKEKERDKEKKKEKEKKKSPKPKKKKHRKEKEERTKGKKKSKKQPPGSEEAAGEPVQNGA; PEEEQLPPESSYSLLAENSYVKMTCDIRGSLQEDSQVTVAIVLENRSSSILKGMELSVLDSLNARMARPQ; GSSVHDGVPVPFQLPPGVSNEAQYVFTIQSIVMAQKLKGTLSFIAKNDEGATHEKLDFRLHFSCSSYLIT; TPCYSDAFAKLLESGDLSMSSIKVDGIRMSFQNLLAKICFHHHFSVVERVDSCASMYSRSIQGHHVCLLV; KKGENSVSVDGKCSDSTLLSNLLEEMKATLAKC; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003938
• ORF Size: 3459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003938.8
• RefSeq Size: 4863 bp
• RefSeq ORF: 3462 bp
• Locus ID: 8943
• UniProt ID: O14617
• Cytogenetics: 19p13.3
• Domains: Adaptin_N
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 130 kDa
• Summary: The protein encoded by this gene is a subunit of the AP3 adaptor-like complex, which is not clathrin-associated, but is associated with the golgi region, as well as more peripheral structures. The AP-3 complex facilitates the budding of vesicles from the golgi membrane, and may be directly involved in trafficking to lysosomes. This subunit is implicated in intracellular biogenesis and trafficking of pigment granules, and possibly platelet dense granules and neurotransmitter vesicles. Defects in this gene are a cause of a new type of Hermansky-Pudlak syndrome. [provided by RefSeq, Feb 2017]