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CDC14A (NM_003672) Human Tagged ORF Clone

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SKU
RC219324
CDC14A (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $553.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CDC14A
Synonyms cdc14; DFNB32; DFNB35; DFNB105; hCDC14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219324 representing NM_003672
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTACTT
TAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGCTGGTCTATGAAAA
TTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGATATTGCTGCAAACTAAACAAGAAA
CTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGCACTACACCTGTTTTGACCAACGGAAAAGAGCAA
ATGCAGCATTTTTGATAGGTGCCTATGCAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGC
ACTCCTGTCTGGCTCAAACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAAT
CTCACCATTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGACAT
TTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGATTGTTCCAGGAAA
ATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATGGTTATCCTCTTCACGCCCCTGAA
GCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTGCAGTTGTGAGGCTAAACAAAAAGATTTATGAGG
CAAAGCGCTTCACAGACGCTGGCTTCGAGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGA
CAACATCGTGCGAAGGTTCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCT
GGTCTTGGAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTGAAA
TAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACTTCCTGGAAGAAAA
ACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGAAAAATCGACCATCCAGTGAAGGA
AGTATTAATAAAATTCTTTCTGGCCTAGATGATATGTCTATTGGTGGAAATCTTTCAAAAACACAAAACA
TGGAACGATTTGGAGAGGATAACTTAGAAGATGATGATGTGGAAATGAAAAATGGTATAACCCAGGGAGA
CAAACTACGTGCCTTAAAAAGTCAGAGACAGCCACGTACCTCACCATCCTGTGCATTTAGGTCAGATGAT
ACAAAAGGACATCCAAGAGCAGTGTCCCAGCCTTTCAGATTAAGTTCATCCCTGCAAGGATCTGCAGTTA
CTTTGAAGACATCAAAAATGGCACTGTCCCCTTCAGCAACGGCCAAGAGGATCAACAGAACTTCTTTGTC
TTCGGGTGCCACTGTAAGAAGCTTTTCCATAAACTCCCGGCTAGCCAGTTCTCTAGGGAACTTGAATGCT
GCAACAGATGATCCAGAGAACAAAAAGACCTCCTCATCCTCTAAGGCAGGCTTCACAGCCAGCCCGTTTA
CCAACCTCTTGAATGGCAGCTCCCAGCCAACTACCAGAAATTACCCTGAGCTCAACAATAATCAGTACAA
CAGAAGCAGCAACAGCAACGGGGGCAACCTGAACAGCCCCCCAGGCCCCCACAGCGCCAAGACAGAGGAG
CACACCACCATCCTCCGACCCTCCTACACCGGGCTTTCTTCTTCTTCAGCGAGATTCCTGAGCCGTTCTA
TCCCTTCCCTTCAGTCTGAATATGTTCATTAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219324 representing NM_003672
Red=Cloning site Green=Tags(s)

MAAESGELIGACEFMKDRLYFATLRNRPKSTVNTHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK
LKSYSLSRKKIVHYTCFDQRKRANAAFLIGAYAVIYLKKTPEEAYRALLSGSNPPYLPFRDASFGNCTYN
LTILDCLQGIRKGLQHGFFDFETFDVDEYEHYERVENGDFNWIVPGKFLAFSGPHPKSKIENGYPLHAPE
AYFPYFKKHNVTAVVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNIVRRFLNICENTEGAIAVHCKA
GLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLEEKQASLWVQGDIFRSKLKNRPSSEG
SINKILSGLDDMSIGGNLSKTQNMERFGEDNLEDDDVEMKNGITQGDKLRALKSQRQPRTSPSCAFRSDD
TKGHPRAVSQPFRLSSSLQGSAVTLKTSKMALSPSATAKRINRTSLSSGATVRSFSINSRLASSLGNLNA
ATDDPENKKTSSSSKAGFTASPFTNLLNGSSQPTTRNYPELNNNQYNRSSNSNGGNLNSPPGPHSAKTEE
HTTILRPSYTGLSSSSARFLSRSIPSLQSEYVHY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003672
ORF Size 1782 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003672.4
RefSeq Size 4262 bp
RefSeq ORF 1785 bp
Locus ID 8556
UniProt ID Q9UNH5, Q59EF4
Cytogenetics 1p21.2
Domains DSPc, PTPc_motif, Y_phosphatase
Protein Families Druggable Genome, Phosphatase
Protein Pathways Cell cycle
MW 66.4 kDa
Summary The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2008
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Documents
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"NM_003672" in other vectors (4)
SKU Description Size Price
RC219324L3 Lenti-ORF clone of CDC14A (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 1 10 ug
$853.00
RC219324L4 Lenti-ORF clone of CDC14A (mGFP-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 1 10 ug
$853.00
RG219324 CDC14A (tGFP-tagged) - Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 1 10 ug
$753.00
SC314134 CDC14A (untagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 1 10 ug
$609.00

Plasmid Map for RC219324

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219324
Circular map for RC219324

file-alt Citations

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