OSTA (SLC51A) (NM_152672) Human Tagged ORF Clone

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SKU
RC219313
SLC51A (Myc-DDK-tagged)-Human organic solute transporter alpha (OSTalpha)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol OSTA
Synonyms OSTA; OSTalpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219313 representing NM_152672
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGGGCAGGACCCAGATAAAGCTTGACCCCAGGTACACAGCAGATCTTCTGGAGGTGCTGAAGA
CCAATTACGGCATCCCCTCCGCCTGCTTCTCTCAGCCTCCCACAGCAGCCCAACTCCTGAGAGCCCTGGG
CCCTGTGGAACTTGCCCTCACTAGCATCCTGACCTTGCTGGCGCTGGGCTCCATTGCCATCTTCCTGGAG
GATGCCGTCTACCTGTACAAGAACACCCTTTGCCCCATCAAGAGGCGGACTCTGCTCTGGAAGAGCTCGG
CACCCACGGTGGTGTCTGTGCTGTGCTGCTTTGGTCTCTGGATCCCTCGTTCCCTGGTGCTGGTGGAAAT
GACCATCACCTCGTTTTATGCCGTGTGCTTTTACCTGCTGATGCTGGTCATGGTGGAAGGCTTTGGGGGG
AAGGAGGCAGTGCTGAGGACGCTGAGGGACACCCCGATGATGGTCCACACAGGCCCCTGCTGCTGCTGCT
GCCCCTGCTGTCCACGGCTGCTGCTCACCAGGAAGAAGCTTCAGCTGCTGATGTTGGGCCCTTTCCAATA
CGCCTTCTTGAAGATAACGCTGACCCTGGTGGGCCTGTTTCTCATCCCCGACGGCATCTATGACCCAGCA
GACATTTCTGAGGGGAGCACAGCTCTATGGATCAACACTTTCCTCGGCGTGTCCACACTGCTGGCTCTCT
GGACCCTGGGCATCATTTCCCGTCAAGCCAGGCTACACCTGGGTGAGCAGAACATGGGAGCCAAATTTGC
TCTGTTCCAGGTTCTCCTCATCCTGACTGCCCTACAGCCCTCCATCTTCTCAGTCTTGGCCAACGGTGGG
CAGATTGCTTGTTCGCCTCCCTATTCCTCTAAAACCAGGTCTCAAGTGATGAATTGCCACCTCCTCATAC
TGGAGACTTTTCTAATGACTGTGCTGACACGAATGTACTACCGAAGGAAAGACCACAAGGTTGGGTATGA
AACTTTCTCTTCTCCAGACCTGGACTTGAACCTCAAAGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC219313 representing NM_152672
Red=Cloning site Green=Tags(s)

MEPGRTQIKLDPRYTADLLEVLKTNYGIPSACFSQPPTAAQLLRALGPVELALTSILTLLALGSIAIFLE
DAVYLYKNTLCPIKRRTLLWKSSAPTVVSVLCCFGLWIPRSLVLVEMTITSFYAVCFYLLMLVMVEGFGG
KEAVLRTLRDTPMMVHTGPCCCCCPCCPRLLLTRKKLQLLMLGPFQYAFLKITLTLVGLFLIPDGIYDPA
DISEGSTALWINTFLGVSTLLALWTLGIISRQARLHLGEQNMGAKFALFQVLLILTALQPSIFSVLANGG
QIACSPPYSSKTRSQVMNCHLLILETFLMTVLTRMYYRRKDHKVGYETFSSPDLDLNLKA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152672
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152672.6
RefSeq Size 1476 bp
RefSeq ORF 1023 bp
Locus ID 200931
UniProt ID Q86UW1
Cytogenetics 3q29
Protein Families Transmembrane
MW 37.6 kDa
Summary Essential component of the Ost-alpha/Ost-beta complex, a heterodimer that acts as the intestinal basolateral transporter responsible for bile acid export from enterocytes into portal blood. Efficiently transports the major species of bile acids.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_152672" in other vectors (6)
SKU Description Size Price
RC219313L1 Lenti ORF clone of Human organic solute transporter alpha (OSTalpha), Myc-DDK-tagged 10 ug
$757.00
RC219313L2 Lenti ORF clone of Human organic solute transporter alpha (OSTalpha), mGFP tagged 10 ug
$757.00
RC219313L3 Lenti ORF clone of Human organic solute transporter alpha (OSTalpha), Myc-DDK-tagged 10 ug
$757.00
RC219313L4 Lenti ORF clone of Human organic solute transporter alpha (OSTalpha), mGFP tagged 10 ug
$757.00
RG219313 SLC51A (tGFP-tagged) - Human organic solute transporter alpha (OSTalpha) 10 ug
$489.00 $657.00
SC100623 SLC51A (untagged)-Human organic solute transporter alpha (OSTalpha) 10 ug
$457.00

Plasmid Map for RC219313

Download sequence for

Cloning scheme for RC219313

Circular map for RC219313

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