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AMN (NM_030943) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC219248
AMN (Myc-DDK-tagged)-Human amnionless homolog (mouse) (AMN)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "AMN or NM_030943"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol AMN
Synonyms amnionless; IGS2; PRO1028
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219248 representing NM_030943
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGGGCGTCCTGGGCCGGGTCCTGCTGTGGCTGCAGCTCTGCGCACTGACCCAGGCGGTC
TCCAAACTCTGGGTCCCCAACACGGACTTCGACGTCGCAGCCAACTGGAGCCAGAACCGG
ACCCCGTGCGCCGGCGGCGCCGTTGAGTTCCCGGCGGACAAGATGGTGTCAGTCCTGGTG
CAAGAAGGTCACGCCGTCTCAGACATGCTCCTGCCGCTGGATGGGGAACTCGTCCTGGCT
TCAGGAGCCGGATTCGGCGTCTCAGACGTGGGCTCGCACCTGGACTGTGGCGCGGGCGAA
CCTGCCGTCTTCCGCGACTCTGACCGCTTCTCCTGGCATGACCCGCACCTGTGGCGCTCT
GGGGACGAGGCACCTGGCCTCTTCTTCGTGGACGCCGAGCGCGTGCCCTGCCGCCACGAC
GACGTCTTCTTTCCGCCTAGTGCCTCCTTCCGCGTGGGGCTCGGCCCTGGCGCTAGCCCC
GTGCGTGTCCGCAGCATCTCGGCTCTGGGCCGGACGTTCACGCGCGACGAGGACCTGGCT
GTTTTCCTGGCGTCCCGCGCGGGCCGCCTACGCTTCCACGGGCCGGGCGCGCTGAGCGTG
GGCCCCGAGGACTGCGCGGACCCGTCGGGCTGCGTCTGCGGCAACGCGGAGGCGCAGCCG
TGGATCTGCGCGGCCCTGCTCCAGCCCCTGGGCGGCCGCTGCCCCCAGGCCGCCTGCCAC
AGCGCCCTCCGGCCCCAGGGGCAGTGCTGTGACCTCTGTGGAGCCGTTGTGTTGCTGACC
CACGGCCCCGCATTTGACCTGGAGCGGTACCGGGCGCGGATACTGGACACCTTCCTGGGT
CTGCCTCAGTACCACGGGCTGCAGGTGGCCGTGTCCAAGGTGCCACGCTCGTCCCGGCTC
CGTGAGGCCGATACGGAGATCCAGGTGGTGCTGGTGGAGAATGGGCCCGAGACAGGCGGA
GCGGGGCGGCTGGCCCGGGCCCTCCTGGCGGACGTCGCCGAGAACGGCGAGGCCCTCGGC
GTCCTGGAGGCGACCATGCGGGAGTCGGGCGCACACGTCTGGGGCAGCTCCGCGGCTGGG
CTGGCGGGCGGCGTGGCGGCTGCCGTGCTGCTGGCGCTGCTGGTCCTGCTGGTGGCGCCG
CCGCTGCTGCGCCGCGCGGGGAGGCTCAGGTGGAGGAGGCACGAGGCGGCGGCCCCGGCT
GGAGCGCCCCTCGGCTTCCGCAACCCGGTGTTCGACGTGACGGCCTCCGAGGAGCTGCCC
CTGCCGCGGCGGCTCAGCCTGGTTCCGAAGGCGGCCGCAGACAGCACCAGCCACAGTTAC
TTCGTCAACCCTCTGTTCGCCGGGGCCGAGGCCGAGGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGAT
GACGACGATAAGGTTTAAA
Protein Sequence
>RC219248 protein sequence
Blue=ORF Red=Cloning site Green=Tag(s)

MGVLGRVLLWLQLCALTQAVSKLWVPNTDFDVAANWSQNRTPCAGGAVEFPADKMVSVLV
QEGHAVSDMLLPLDGELVLASGAGFGVSDVGSHLDCGAGEPAVFRDSDRFSWHDPHLWRS
GDEAPGLFFVDAERVPCRHDDVFFPPSASFRVGLGPGASPVRVRSISALGRTFTRDEDLA
VFLASRAGRLRFHGPGALSVGPEDCADPSGCVCGNAEAQPWICAALLQPLGGRCPQAACH
SALRPQGQCCDLCGAVVLLTHGPAFDLERYRARILDTFLGLPQYHGLQVAVSKVPRSSRL
READTEIQVVLVENGPETGGAGRLARALLADVAENGEALGVLEATMRESGAHVWGSSAAG
LAGGVAAAVLLALLVLLVAPPLLRRAGRLRWRRHEAAAPAGAPLGFRNPVFDVTASEELP
LPRRLSLVPKAAADSTSHSYFVNPLFAGAEAEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030943
ORF Size 1359 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030943.4
RefSeq Size 1896 bp
RefSeq ORF 1362 bp
Locus ID 81693
UniProt ID Q9BXJ7
Cytogenetics 14q32.32
Protein Families Druggable Genome, Transmembrane
MW 45.6 kDa
Summary The protein encoded by this gene is a type I transmembrane protein. It is thought to modulate bone morphogenetic protein (BMP) receptor function by serving as an accessory or coreceptor, and thus facilitates or hinders BMP binding. It is known that the mouse AMN gene is expressed in the extraembryonic visceral endoderm layer during gastrulation, but it is found to be mutated in amnionless mouse. The encoded protein has sequence similarity to short gastrulation (Sog) and procollagen IIA proteins in Drosophila. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_030943" in other vectors (6)
SKU Description Size Price
RC219248L1 Lenti ORF clone of Human amnionless homolog (mouse) (AMN), Myc-DDK-tagged 10 ug
$757.00
RC219248L2 Lenti ORF clone of Human amnionless homolog (mouse) (AMN), mGFP tagged 10 ug
$757.00
RC219248L3 Lenti ORF clone of Human amnionless homolog (mouse) (AMN), Myc-DDK-tagged 10 ug
$757.00
RC219248L4 Lenti ORF clone of Human amnionless homolog (mouse) (AMN), mGFP tagged 10 ug
$757.00
RG219248 AMN (tGFP-tagged) - Human amnionless homolog (mouse) (AMN) 10 ug
$489.00 $657.00
SC305303 AMN (untagged)-Human amnionless homolog (mouse) (AMN) 10 ug
$457.00

Plasmid Map for RC219248

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219248
Circular map for RC219248

file-alt Citations

Amnionless-mediated glycosylation is crucial for cell surface targeting of cubilin in renal and intestinal cells
Udagawa, Harita, Miura et al
Sci Rep (2018) 8 (1), 2351 DOI: 10.1038/s41598-018-20731-4
AI Summary: OriGene products contributed to this study by providing expression vectors, specifically plasmids containing full-length human amnionless cDNA with a C-terminal mycDDK tag, which were used to analyze the role of amnionless in cubilin membrane targeting.
Snippet: microscope. Images were processed using Adobe Photoshop CS6. Expression vectors Plasmids containing full length cDNA for wild-type human amnionless with the C-terminal mycDDK tag were purchased from OriGene Technologies (Rockville, MD). The mini-cubilin plasmid coding region of rat cubilin including 1–2784 bp cDNA (1–928AA) was amplified by PCR using the following specific primer sets: 5′
FigureFigureFigure
Showing 1-1 of 1 papers.
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