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CHIT1 (NM_003465) Human Tagged ORF Clone

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SKU
RC219169
CHIT1 (Myc-DDK-tagged)-Human chitinase 1 (chitotriosidase) (CHIT1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CHIT1
Synonyms CHI3; CHIT; CHITD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219169 representing NM_003465
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCGGTCTGTGGCCTGGGCAGGTTTCATGGTCCTGCTGATGATCCCATGGGGCTCTGCTGCAAAAC
TGGTCTGCTACTTCACCAACTGGGCCCAGTACAGACAGGGGGAGGCTCGCTTCCTGCCCAAGGACTTGGA
CCCCAGCCTTTGCACCCACCTCATCTACGCCTTCGCTGGCATGACCAACCACCAGCTGAGCACCACTGAG
TGGAATGACGAGACTCTCTACCAGGAGTTCAATGGCCTGAAGAAGATGAATCCCAAGCTGAAGACCCTGT
TAGCCATCGGAGGCTGGAATTTCGGCACTCAGAAGTTCACAGATATGGTAGCCACGGCCAACAACCGTCA
GACCTTTGTCAACTCGGCCATCAGGTTTCTGCGCAAATACAGCTTTGACGGCCTTGACCTTGACTGGGAG
TACCCAGGAAGCCAGGGGAGCCCTGCCGTAGACAAGGAGCGCTTCACAACCCTGGTACAGGACTTGGCCA
ATGCCTTCCAGCAGGAAGCCCAGACCTCAGGGAAGGAACGCCTTCTTCTGAGTGCAGCGGTTCCAGCTGG
GCAGACCTATGTGGATGCTGGATACGAGGTGGACAAAATCGCCCAGAACCTGGATTTTGTCAACCTTATG
GCCTACGACTTCCATGGCTCTTGGGAGAAGGTCACGGGACATAACAGCCCCCTCTACAAGAGGCAAGAAG
AGAGTGGTGCAGCAGCCAGCCTCAACGTGGATGCTGCTGTGCAACAGTGGCTGCAGAAGGGGACCCCTGC
CAGCAAGCTGATCCTTGGCATGCCTACCTACGGACGCTCCTTCACACTGGCCTCCTCATCAGACACCAGA
GTGGGGGCCCCAGCCACAGGGTCTGGCACTCCAGGCCCCTTCACCAAGGAAGGAGGGATGCTGGCCTACT
ATGAAGTCTGCTCCTGGAAGGGGGCCACCAAACAGAGAATCCAGGATCAGAAGGTGCCCTACATCTTCCG
GGACAACCAGTGGGTGGGCTTTGATGATGTGGAGAGCTTCAAAACCAAGGTCAGCTATCTGAAGCAGAAG
GGACTGGGCGGGGCCATGGTCTGGGCACTGGACTTAGATGACTTTGCCGGCTTCTCCTGCAACCAGGGCC
GATACCCCCTCATCCAGACGCTACGGCAGGAACTGAGTCTTCCATACTTGCCTTCAGGCACCCCAGAGCT
TGAAGTTCCAAAACCAGGTCAGCCCTCTGAACCTGAGCATGGCCCCAGCCCTGGACAAGACACGTTCTGC
CAGGGCAAAGCTGATGGGCTCTATCCCAATCCTCGGGAACGGTCCAGCTTCTACAGCTGTGCAGCGGGGC
GGCTGTTCCAGCAAAGCTGCCCGACAGGCCTGGTGTTCAGCAACTCCTGCAAATGCTGCACCTGGAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219169 representing NM_003465
Red=Cloning site Green=Tags(s)

MVRSVAWAGFMVLLMIPWGSAAKLVCYFTNWAQYRQGEARFLPKDLDPSLCTHLIYAFAGMTNHQLSTTE
WNDETLYQEFNGLKKMNPKLKTLLAIGGWNFGTQKFTDMVATANNRQTFVNSAIRFLRKYSFDGLDLDWE
YPGSQGSPAVDKERFTTLVQDLANAFQQEAQTSGKERLLLSAAVPAGQTYVDAGYEVDKIAQNLDFVNLM
AYDFHGSWEKVTGHNSPLYKRQEESGAAASLNVDAAVQQWLQKGTPASKLILGMPTYGRSFTLASSSDTR
VGAPATGSGTPGPFTKEGGMLAYYEVCSWKGATKQRIQDQKVPYIFRDNQWVGFDDVESFKTKVSYLKQK
GLGGAMVWALDLDDFAGFSCNQGRYPLIQTLRQELSLPYLPSGTPELEVPKPGQPSEPEHGPSPGQDTFC
QGKADGLYPNPRERSSFYSCAAGRLFQQSCPTGLVFSNSCKCCTWN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_003465
ORF Size 1398 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003465.3
RefSeq Size 1633 bp
RefSeq ORF 1401 bp
Locus ID 1118
UniProt ID Q13231
Cytogenetics 1q32.1
Domains CBM_14, Glyco_18
Protein Families Secreted Protein, Transmembrane
Protein Pathways Amino sugar and nucleotide sugar metabolism
MW 51.5 kDa
Summary Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. provided by RefSeq, Jan 2012
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Documents
Resources
"NM_003465" in other vectors (6)
SKU Description Size Price
RC219169L1 Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), Myc-DDK-tagged 10 ug
$757.00
RC219169L2 Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), mGFP tagged 10 ug
$757.00
RC219169L3 Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), Myc-DDK-tagged 10 ug
$757.00
RC219169L4 Lenti ORF clone of Human chitinase 1 (chitotriosidase) (CHIT1), mGFP tagged 10 ug
$757.00
RG219169 CHIT1 (tGFP-tagged) - Human chitinase 1 (chitotriosidase) (CHIT1) 10 ug
$489.00 $657.00
SC117950 CHIT1 (untagged)-Human chitinase 1 (chitotriosidase) (CHIT1) 10 ug
$457.00

Plasmid Map for RC219169

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219169
Circular map for RC219169

file-alt Citations

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