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UBXN2B (NM_001077619) Human Tagged ORF Clone

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SKU
RC219137
UBXN2B (Myc-DDK-tagged)-Human UBX domain protein 2B (UBXN2B)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UBXN2B
Synonyms p37
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219137 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGGCGGAGGCCCTGAGCCCGGCGAGCAGGAGAGGAGGTCTTCCGGGCCGCGGCCTCCGAGCG
CGCGGGATTTGCAGTTGGCCTTGGCAGAATTGTATGAAGATGAGGTGAAGTGCAAATCTTCCAAGTCTAA
TAGACCTAAAGCCACAGTCTTCAAGAGCCCACGGACACCACCTCAACGGTTTTACTCAAGTGAACATGAA
TACAGTGGATTAAATATAGTTCGACCTTCAACTGGGAAAATTGTGAATGAACTTTTCAAAGAGGCAAGGG
AACATGGGGCTGTCCCTCTGAATGAAGCCACAAGAGCTTCAGGTGATGATAAATCTAAGTCATTTACAGG
TGGAGGATACAGATTGGGTAGTTCTTTTTGTAAGCGGTCTGAATATATCTATGGAGAAAATCAGCTGCAA
GATGTTCAGATTTTGCTTAAACTGTGGAGCAATGGTTTCAGTTTAGATGATGGAGAATTGAGACCTTACA
ATGAACCAACAAATGCTCAATTTCTGGAGTCTGTTAAGAGAGGAGAGATTCCCCTGGAGCTTCAGCGCCT
TGTTCATGGTGGCCAAGTGAATTTGGATATGGAGGATCATCAGGATCAAGAATACATAAAACCTAGATTG
AGGTTCAAGGCTTTTAGTGGAGAAGGGCAAAAACTTGGAAGCCTTACACCTGAAATAGTCAGTACACCTT
CCTCTCCAGAAGAGGAGGATAAATCAATACTTAATGCAGTTGTTCTTATTGATGATTCAGTGCCAACAAC
AAAAATTCAAATCAGGTTAGCAGATGGGAGTCGTTTGATACAAAGATTCAATAGTACACACAGGATCCTG
GATGTCCGGAACTTTATTGTACAGTCTCGTCCTGAATTTGCGGCTCTTGACTTTATTCTTGTGACTTCAT
TTCCGAATAAAGAGCTAACAGATGAAAGCCTGACACTGCTAGAAGCAGATATTCTTAACACTGTGTTACT
CCAGCAACTAAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219137 protein sequence
Red=Cloning site Green=Tags(s)

MAEGGGPEPGEQERRSSGPRPPSARDLQLALAELYEDEVKCKSSKSNRPKATVFKSPRTPPQRFYSSEHE
YSGLNIVRPSTGKIVNELFKEAREHGAVPLNEATRASGDDKSKSFTGGGYRLGSSFCKRSEYIYGENQLQ
DVQILLKLWSNGFSLDDGELRPYNEPTNAQFLESVKRGEIPLELQRLVHGGQVNLDMEDHQDQEYIKPRL
RFKAFSGEGQKLGSLTPEIVSTPSSPEEEDKSILNAVVLIDDSVPTTKIQIRLADGSRLIQRFNSTHRIL
DVRNFIVQSRPEFAALDFILVTSFPNKELTDESLTLLEADILNTVLLQQLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001077619
ORF Size 993 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001077619.1, NP_001071087.1
RefSeq Size 5088 bp
RefSeq ORF 996 bp
Locus ID 137886
UniProt ID Q14CS0
Cytogenetics 8q12.1
MW 37.1 kDa
Summary Adapter protein required for Golgi and endoplasmic reticulum biogenesis (PubMed:17141156). Involved in Golgi and endoplasmic reticulum maintenance during interphase and in their reassembly at the end of mitosis (PubMed:17141156). The complex formed with VCP has membrane fusion activity; membrane fusion activity requires USO1-GOLGA2 tethering and BET1L (PubMed:17141156). VCPIP1 is also required, but not its deubiquitinating activity (PubMed:17141156). Together with NSFL1C/p47, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807).UniProtKB/Swiss-Prot Function
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Documents
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"NM_001077619" in other vectors (6)
SKU Description Size Price
RC219137L1 Lenti ORF clone of Human UBX domain protein 2B (UBXN2B), Myc-DDK-tagged 10 ug
$600.00
RC219137L2 Lenti ORF clone of Human UBX domain protein 2B (UBXN2B), mGFP tagged 10 ug
$600.00
RC219137L3 Lenti ORF clone of Human UBX domain protein 2B (UBXN2B), Myc-DDK-tagged 10 ug
$600.00
RC219137L4 Lenti ORF clone of Human UBX domain protein 2B (UBXN2B), mGFP tagged 10 ug
$600.00
RG219137 UBXN2B (tGFP-tagged) - Human UBX domain protein 2B (UBXN2B) 10 ug
$500.00
SC315531 UBXN2B (untagged)-Human UBX domain protein 2B (UBXN2B) 10 ug
$330.00

Plasmid Map for RC219137

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219137
Circular map for RC219137

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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