LDHAL6A (NM_144972) Human Tagged ORF Clone

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SKU
RC219052
LDHAL6A (Myc-DDK-tagged)-Human lactate dehydrogenase A-like 6A (LDHAL6A), transcript variant 1
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LDHAL6A
Synonyms LDH6A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219052 representing NM_144972
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACTATCAAGAGTGAACTTATTAAGAATTTCGCGGAAGAGGAGGCCATTCATCACAATAAGATCT
CCATTGTAGGAACTGGATCGGTTGGTGTGGCTTGTGCTATCAGCATCTTATTAAAAGGTTTGAGTGATGA
ACTTGTCCTTGTGGATGTTGATGAAGGCAAACTGAAGGGTGAGACAATGGATCTTCAACATGGCAGCCCT
TTTATGAAAATGCCAAATATTGTCTCCAGCAAAGATTACCTGGTCACTGCAAACTCCAATCTAGTGATTA
TCACAGCAGGTGCACGCCAGAAAAAAGGAGAAACACGCCTTGATTTAGTCCAGCGAAATGTATCCATCTT
TAAATTAATGATTCCCAATATTACCCAGTACAGTCCTCACTGCAAACTGCTTATTGTTACTAATCCAGTG
GATATCTTAACTTATGTAGCCTGGAAGTTGAGTGGATTTCCCAAAAACCGTGTTATTGGAAGTGGTTGTA
ATCTGGACTCTGCTCGTTTTCGTTACTTTATTGGGCAAAGGCTTGGCATCCACTCTGAAAGCTGTCATGG
GCTGATCCTTGGAGAGCATGGCGACTCAAGTGTTCCTGTGTGGAGTGGTGTGAACATTGCTGGCGTCCCT
CTGAAGGATCTGAACCCAGATATAGGAACTGATAAAGATCCTGAGCAGTGGGAAAATGTCCACAAAAAAG
TGATTTCCAGTGGCTATGAGATGGTCAAAATGAAAGGTTATACTTCTTGGGGCATTAGCCTATCTGTAGC
TGATTTAACAGAAAGTATTTTGAAGAATCTTAGGAGAGTGCATCCAGTTTCTACCCTAAGTAAGGGCCTC
TATGGAATAAATGAAGACATATTCCTTAGTGTCCCATGTATCCTGGGAGAGAATGGTATCACAGACCTCA
TAAAAGTAAAACTGACTCTTGAAGAGGAGGCCTGCTTGCAAAAGAGTGCAGAAACACTTTGGGAAATTCA
GAAGGAGCTCAAGCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC219052 representing NM_144972
Red=Cloning site Green=Tags(s)

MATIKSELIKNFAEEEAIHHNKISIVGTGSVGVACAISILLKGLSDELVLVDVDEGKLKGETMDLQHGSP
FMKMPNIVSSKDYLVTANSNLVIITAGARQKKGETRLDLVQRNVSIFKLMIPNITQYSPHCKLLIVTNPV
DILTYVAWKLSGFPKNRVIGSGCNLDSARFRYFIGQRLGIHSESCHGLILGEHGDSSVPVWSGVNIAGVP
LKDLNPDIGTDKDPEQWENVHKKVISSGYEMVKMKGYTSWGISLSVADLTESILKNLRRVHPVSTLSKGL
YGINEDIFLSVPCILGENGITDLIKVKLTLEEEACLQKSAETLWEIQKELKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_144972
ORF Size 996 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_144972.5
RefSeq Size 2520 bp
RefSeq ORF 999 bp
Locus ID 160287
UniProt ID Q6ZMR3
Cytogenetics 11p15.1
Protein Pathways Cysteine and methionine metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
MW 36.3 kDa
Summary Displays an lactate dehydrogenase activity. Significantly increases the transcriptional activity of JUN, when overexpressed.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_144972" in other vectors (4)
SKU Description Size Price
RC219052L3 Lenti ORF clone of Human lactate dehydrogenase A-like 6A (LDHAL6A), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC219052L4 Lenti ORF clone of Human lactate dehydrogenase A-like 6A (LDHAL6A), transcript variant 1, mGFP tagged 10 ug
$600.00
RG219052 LDHAL6A (tGFP-tagged) - Human lactate dehydrogenase A-like 6A (LDHAL6A), transcript variant 1 10 ug
$500.00
SC306200 LDHAL6A (untagged)-Human lactate dehydrogenase A-like 6A (LDHAL6A), transcript variant 1 10 ug
$330.00

Plasmid Map for RC219052

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Cloning scheme for RC219052

Circular map for RC219052

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