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FRMD3 (NM_174938) Human Tagged ORF Clone

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SKU
RC219021
FRMD3 (Myc-DDK-tagged)-Human FERM domain containing 3 (FRMD3)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $556.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FRMD3
Synonyms 4.1O; EPB41L4O; EPB41LO; P410
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219021 representing NM_174938
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCGCCTCCTGCCACTGTGTGCCGAGAGGCAGGAGGACCATGAAAATGATCCACTTTCGGAGCTCCA
GCGTCAAATCGCTCAGCCAGGAGATGAGATGCACCATCCGGCTGCTGGACGACTCGGAGATCTCCTGCCA
CATCCAGAGGGAAACCAAAGGGCAGTTTCTCATTGACCACATCTGCAACTACTACAGCCTGCTGGAGAAG
GACTACTTTGGCATTCGCTATGTGGACCCAGAGAAGCAAAGGCACTGGCTTGAACCTAACAAGTCCATCT
TCAAGCAAATGAAAACTCATCCACCATACACCATGTGCTTTAGAGTGAAATTCTACCCACATGAACCCTT
GAAGATTAAAGAAGAGCTCACAAGATACCTTTTATACCTTCAGATTAAAAGGGACATTTTTCATGGCCGC
CTGCTGTGCTCCTTTTCTGATGCTGCCTACCTGGGTGCCTGTATTGTTCAAGCTGAGCTTGGTGATTACG
ATCCTGATGAGCATCCTGAGAATTACATCAGTGAGTTTGAGATTTTCCCCAAGCAGTCACAGAAGCTGGA
AAGAAAAATAGTGGAAATTCATAAAAATGAACTCAGGGGGCAGAGCCCACCAGTTGCTGAATTTAACTTG
CTCCTGAAAGCTCACACTTTGGAAACCTACGGGGTGGATCCTCACCCATGCAAGGATTCAACAGGCACAA
CAACATTTTTAGGATTCACAGCTGCAGGCTTTGTGGTCTTTCAGGGAAATAAGAGAATCCATTTGATAAA
ATGGCCAGATGTCTGCAAATTGAAGTTTGAAGGGAAGACATTTTATGTGATTGGCACCCAGAAGGAGAAA
AAAGCCATGTTGGCATTCCATACTTCAACACCAGCTGCCTGCAAACATCTTTGGAAGTGTGGAGTGGAAA
ACCAGGCCTTTTATAAGTATGCAAAATCCAGTCAGATCAAGACTGTATCAAGCAGCAAGATATTTTTTAA
AGGAAGTAGATTTCGATATAGTGGGAAAGTTGCCAAAGAGGTGGTGGAGGCCAGTTCCAAGATCCAGAGG
GAGCCTCCTGAGGTGCACAGAGCCAACATTACTCAGAGCCGCAGTTCCCACTCCTTGAACAAACAGCTCA
TCATTAACATGGAACCCCTGCAGCCCCTGCTTCCTTCCCCCAGCGAGCAAGAAGAAGAACTTCCTCTGGG
TGAGGGTGTTCCATTGCCTAAAGAGGAGAACATTTCTGCTCCCTTGATCTCCAGCTCCCCAGTGAAGGCA
GCCCGGGAGTATGAAGATCCCCCTAGTGAAGAGGAAGATAAAATAAAAGAAGAACCTTTAACCATCTCTG
AACTAGTGTACAACCCAAGTGCCAGCCTGCTCCCCACCCCTGTGGATGACGATGAGATTGACATGCTCTT
TGACTGTCCTTCTAGGCTTGAGTTGGAAAGAGAAGACACAGATTCATTTGAGGATCTGGAAGCAGATGAA
AACGCCTTTTTGATTGCTGAAGAAGAGGAGCTGAAGGAGGCTCGCCGTGCTTTGTCGTGGAGCTATGACA
TTCTGACTGGCCATATTCGGGTGAACCCACTGGTCAAGAGTTTTTCCAGGCTCCTTGTGGTGGGCCTGGG
ACTGCTGCTCTTTGTATTTCCCCTGCTCCTCCTCCTTTTGGAGTCAGGTATTGATCTCTCCTTCTTATGC
GAAATCCGCCAGACACCAGAGTTTGAGCAGTTTCACTATGAATACTACTGTCCCCTCAAGGAGTGGGTGG
CTGGGAAAGTCCACCTCATCCTCTACATGCTGGGTTGCTCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC219021 representing NM_174938
Red=Cloning site Green=Tags(s)

MFASCHCVPRGRRTMKMIHFRSSSVKSLSQEMRCTIRLLDDSEISCHIQRETKGQFLIDHICNYYSLLEK
DYFGIRYVDPEKQRHWLEPNKSIFKQMKTHPPYTMCFRVKFYPHEPLKIKEELTRYLLYLQIKRDIFHGR
LLCSFSDAAYLGACIVQAELGDYDPDEHPENYISEFEIFPKQSQKLERKIVEIHKNELRGQSPPVAEFNL
LLKAHTLETYGVDPHPCKDSTGTTTFLGFTAAGFVVFQGNKRIHLIKWPDVCKLKFEGKTFYVIGTQKEK
KAMLAFHTSTPAACKHLWKCGVENQAFYKYAKSSQIKTVSSSKIFFKGSRFRYSGKVAKEVVEASSKIQR
EPPEVHRANITQSRSSHSLNKQLIINMEPLQPLLPSPSEQEEELPLGEGVPLPKEENISAPLISSSPVKA
AREYEDPPSEEEDKIKEEPLTISELVYNPSASLLPTPVDDDEIDMLFDCPSRLELEREDTDSFEDLEADE
NAFLIAEEEELKEARRALSWSYDILTGHIRVNPLVKSFSRLLVVGLGLLLFVFPLLLLLLESGIDLSFLC
EIRQTPEFEQFHYEYYCPLKEWVAGKVHLILYMLGCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_174938
ORF Size 1791 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_174938.6
RefSeq Size 2529 bp
RefSeq ORF 1794 bp
Locus ID 257019
UniProt ID A2A2Y4
Cytogenetics 9q21.32
Protein Families Transmembrane
MW 68.6 kDa
Summary The protein encoded by this gene is a single pass membrane protein primarily found in ovaries. A similar protein in erythrocytes helps determine the shape of red blood cells, but the function of the encoded protein has not been determined. There is some evidence that this is a tumor suppressor gene, and there is also evidence linking defects in this gene to susceptibility to diabetic nephropathy in type 1 diabetes. Several transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011
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Documents
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"NM_174938" in other vectors (6)
SKU Description Size Price
RC219021L1 Lenti-ORF clone of FRMD3 (Myc-DDK-tagged)-Human FERM domain containing 3 (FRMD3) 10 ug
$856.00
RC219021L2 Lenti-ORF clone of FRMD3 (mGFP-tagged)-Human FERM domain containing 3 (FRMD3) 10 ug
$856.00
RC219021L3 Lenti-ORF clone of FRMD3 (Myc-DDK-tagged)-Human FERM domain containing 3 (FRMD3) 10 ug
$856.00
RC219021L4 Lenti-ORF clone of FRMD3 (mGFP-tagged)-Human FERM domain containing 3 (FRMD3) 10 ug
$856.00
RG219021 FRMD3 (tGFP-tagged) - Human FERM domain containing 3 (FRMD3) 10 ug
$756.00
SC318030 FRMD3 (untagged)-Human FERM domain containing 3 (FRMD3) 10 ug
$612.00

Plasmid Map for RC219021

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC219021
Circular map for RC219021

file-alt Citations

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