GAB1 (NM_002039) Human Tagged ORF Clone

SKU
RC219005
GAB1 (Myc-DDK-tagged)-Human GRB2-associated binding protein 1 (GAB1), transcript variant 2
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GAB1
Synonyms DFNB26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC219005 representing NM_002039
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGTGGTGAAGTGGTCTGCTCCGGATGGCTCCGCAAGTCCCCCCCGGAGAAAAAGTTGAAGCGTT
ATGCATGGAAGAGGAGATGGTTCGTGTTACGCAGTGGCCGTTTAACTGGAGATCCAGATGTTTTGGAATA
TTACAAAAATGATCATGCCAAGAAGCCTATTCGTATTATTGATTTAAATTTATGTCAACAAGTAGATGCT
GGATTGACATTTAACAAAAAAGAGTTTGAAAACAGCTACATTTTTGATATCAACACTATTGACCGGATTT
TCTACTTGGTAGCAGACAGCGAGGAGGAGATGAATAAGTGGGTTCGTTGTATTTGTGACATCTGTGGGTT
TAATCCAACAGAAGAAGATCCTGTGAAGCCACCTGGCAGCTCTTTACAAGCACCAGCTGATTTACCTTTA
GCTATAAATACAGCACCACCATCCACCCAGGCAGATTCATCCTCTGCTACTCTACCTCCTCCATATCAGC
TAATCAATGTTCCACCACACCTGGAAACTCTTGGCATTCAGGAGGATCCTCAAGACTACCTGTTGCTCAT
CAACTGTCAAAGCAAGAAGCCCGAACCCACCAGAACGCATGCTGATTCTGCAAAATCCACCTCTTCTGAA
ACAGACTGCAATGATAACGTCCCTTCTCATAAAAATCCTGCTTCCTCCCAGAGCAAACATGGAATGAATG
GCTTTTTTCAGCAGCAAATGATATACGACTCTCCACCTTCACGTGCCCCATCTGCTTCAGTTGACTCCAG
CCTTTATAACCTGCCCAGGAGTTATTCCCATGATGTTTTACCAAAGGTGTCTCCATCAAGTACTGAAGCA
GATGGAGAACTCTATGTTTTTAATACCCCATCTGGGACATCGAGTGTAGAGACTCAAATGAGGCATGTAT
CTATTAGTTATGACATTCCTCCAACACCTGGTAATACTTATCAGATTCCACGAACATTTCCAGAAGGAAC
CTTGGGACAGACATCAAAGCTAGACACTATTCCAGATATTCCTCCACCTCGGCCACCGAAACCACATCCA
GCTCATGACCGATCTCCTGTGGAAACGTGTAGTATCCCACGCACCGCCTCAGACACTGACAGTAGTTACT
GTATCCCTACAGCAGGGATGTCGCCTTCACGTAGTAATACCATTTCCACTGTGGATTTAAACAAATTGCG
AAAAGATGCTAGTTCTCAAGACTGCTATGATATTCCACGAGCATTTCCAAGTGATAGATCTAGTTCACTT
GAAGGCTTCCATAACCACTTTAAAGTCAAAAATGTGTTGACAGTGGGAAGTGTTTCAAGTGAAGAACTGG
ATGAAAATTACGTCCCAATGAATCCCAATTCACCACCACGACAACATTCCAGCAGTTTTACAGAACCAAT
TCAGGAAGCAAATTATGTGCCAATGACTCCAGGAACATTTGATTTTTCCTCATTTGGAATGCAAGTTCCT
CCTCCTGCTCATATGGGCTTCAGGTCCAGCCCAAAAACCCCTCCCAGAAGGCCAGTTCCTGTTGCAGACT
GTGAACCACCCCCCGTGGATAGGAACCTCAAGCCAGACAGAAAAGTCAAGCCAGCGCCTTTAGAAATAAA
ACCTTTGCCAGAATGGGAAGAATTACAAGCCCCAGTTAGATCTCCCATCACTAGGAGTTTTGCTCGAGAC
TCTTCCAGGTTTCCCATGTCCCCCCGACCAGATTCAGTGCATAGCACAACTTCAAGCAGTGACTCACACG
ACAGTGAAGAGAATTATGTTCCCATGAACCCAAACCTGTCCAGTGAAGACCCAAATCTCTTTGGCAGTAA
CAGTCTTGATGGAGGAAGCAGCCCTATGATCAAGCCCAAAGGAGACAAACAGGTGGAATACTTAGATCTC
GACTTAGATTCTGGGAAATCCACACCACCACGTAAGCAAAAGAGCAGTGGCTCAGGCAGCAGTGTAGCAG
ATGAGAGAGTGGATTATGTTGTTGTTGACCAACAGAAGACCTTGGCTCTAAAGAGTACCCGGGAAGCCTG
GACAGATGGGAGACAGTCCACAGAATCAGAAACGCCAGCGAAGAGTGTGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC219005 representing NM_002039
Red=Cloning site Green=Tags(s)

MSGGEVVCSGWLRKSPPEKKLKRYAWKRRWFVLRSGRLTGDPDVLEYYKNDHAKKPIRIIDLNLCQQVDA
GLTFNKKEFENSYIFDINTIDRIFYLVADSEEEMNKWVRCICDICGFNPTEEDPVKPPGSSLQAPADLPL
AINTAPPSTQADSSSATLPPPYQLINVPPHLETLGIQEDPQDYLLLINCQSKKPEPTRTHADSAKSTSSE
TDCNDNVPSHKNPASSQSKHGMNGFFQQQMIYDSPPSRAPSASVDSSLYNLPRSYSHDVLPKVSPSSTEA
DGELYVFNTPSGTSSVETQMRHVSISYDIPPTPGNTYQIPRTFPEGTLGQTSKLDTIPDIPPPRPPKPHP
AHDRSPVETCSIPRTASDTDSSYCIPTAGMSPSRSNTISTVDLNKLRKDASSQDCYDIPRAFPSDRSSSL
EGFHNHFKVKNVLTVGSVSSEELDENYVPMNPNSPPRQHSSSFTEPIQEANYVPMTPGTFDFSSFGMQVP
PPAHMGFRSSPKTPPRRPVPVADCEPPPVDRNLKPDRKVKPAPLEIKPLPEWEELQAPVRSPITRSFARD
SSRFPMSPRPDSVHSTTSSSDSHDSEENYVPMNPNLSSEDPNLFGSNSLDGGSSPMIKPKGDKQVEYLDL
DLDSGKSTPPRKQKSSGSGSSVADERVDYVVVDQQKTLALKSTREAWTDGRQSTESETPAKSVK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_002039
ORF Size 2082 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002039.4
RefSeq Size 3323 bp
RefSeq ORF 2085 bp
Locus ID 2549
UniProt ID Q13480
Cytogenetics 4q31.21
Domains PH
Protein Families Druggable Genome
Protein Pathways ErbB signaling pathway, Neurotrophin signaling pathway, Renal cell carcinoma
MW 76.4 kDa
Summary The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
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SKU Description Size Price
RC219005L1 Lenti ORF clone of Human GRB2-associated binding protein 1 (GAB1), transcript variant 2, Myc-DDK-tagged 10 ug
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RC219005L3 Lenti ORF clone of Human GRB2-associated binding protein 1 (GAB1), transcript variant 2, Myc-DDK-tagged 10 ug
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RC219005L4 Lenti ORF clone of Human GRB2-associated binding protein 1 (GAB1), transcript variant 2, mGFP tagged 10 ug
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Citations

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