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PDE5A (NM_033430) Human Tagged ORF Clone

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SKU
RC218974
PDE5A (Myc-DDK-tagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $763.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PDE5A
Synonyms CGB-PDE; CN5A; PDE5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218974 representing NM_033430
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGCCCTTTGGAGACAAAACAAGAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCC
CTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGC
AGATAACAGTGTCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCC
ATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTC
TAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATAT
TTCTAGTCATTTGGATGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCT
GACCGCTATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTG
ATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCAT
TGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAAT
GCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGG
AAGAGGTTGTTGGTGTAGCCCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGA
TGAAAAGGACTTTGCTGCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACT
TCACTGCTGGAGAACAAGAGAAATCAGGTGCTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAAT
CATTAGAAGTAATTTTGAAGAAAATAGCTGCCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCAT
TTTCATAGTGGATGAAGATTGCTCCGATTCTTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAA
AAATCATCTGATACATTAACAAGGGAACATGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCA
AAAATACTATGGAACCACTTAATATCCCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAA
TACAGGAAATGTAAACCAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAAT
AAAGTTATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACC
GAAATGACGAACAGTTTCTGGAAGCTTTTGTCATCTTTTGTGGCTTGGGTATCCAGAACACGCAGATGTA
TGAAGCAGTGGAGAGAGCCATGGCCAAGCAAATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCA
GCAGAGGAAGAAACAAGAGAGCTACAGTCGTTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAA
TTACTGACTTTAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTT
TACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTT
AAGAAGAATTATCGGAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGT
TTGCTGCTCTAAAAGCAGGCAAAATTCAGAACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGC
TGCACTAAGCCACGATTTGGATCACCGTGGTGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTT
GCCCAGCTTTACTGCCATTCAATCATGGAACACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTC
CAGGCAATCAGATTCTCAGTGGCCTCTCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGC
TATTTTAGCTACAGACCTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAAT
CAATTCAATTTGGAAGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTT
CTGCAATTACAAAACCCTGGCCTATTCAACAACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCA
AGGAGACAGAGAGAGAAAAGAACTCAACATAGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAA
ATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGT
CAGAGGACTGTTTCCCTTTACTAGATGGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACA
GCAGGAGAAGATGCTGATTAATGGGGAAAGCGGCCAGGCCAAGCGGAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218974 protein sequence
Red=Cloning site Green=Tags(s)

MLPFGDKTREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSVPGTPTRKISASEFDRPLRP
IVVKDSEGTVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISA
DRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFN
AEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYET
SLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELE
KSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKN
KVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASA
AEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSV
KKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPL
AQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKN
QFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNK
IPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_033430
ORF Size 2499 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_033430.1
RefSeq Size 6836 bp
RefSeq ORF 2502 bp
Locus ID 8654
UniProt ID O76074
Cytogenetics 4q26
Protein Families Druggable Genome
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
MW 94.6 kDa
Summary This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. provided by RefSeq, Jul 2008
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"NM_033430" in other vectors (6)
SKU Description Size Price
RC218974L1 Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,063.00
RC218974L2 Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, mGFP tagged 10 ug
$1,063.00
RC218974L3 Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, Myc-DDK-tagged 10 ug
$1,063.00
RC218974L4 Lenti ORF clone of Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2, mGFP tagged 10 ug
$1,063.00
RG218974 PDE5A (tGFP-tagged) - Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2 10 ug
$789.00 $963.00
SC120663 PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2 10 ug
$764.00

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