RBIS (NM_001099671) Human Tagged ORF Clone

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SKU
RC218877
C8orf59 (Myc-DDK-tagged)-Human chromosome 8 open reading frame 59 (C8orf59), transcript variant 2
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RBIS
Synonyms C8orf59
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218877 representing NM_001099671
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAGAACAAATTAAGAGGGCCGAAGTCCAGGAATGTATTTCACATAGCCAGCCAAAAAAACTTTA
AGGCTAAAAACAAAGCAAAACCAGTTACCACTAATCTTAAGAAGATAAACATTATGAATGAGGAAAAAGT
TAACAGAGTAAATAAAGCTTTTGTAAATGTACAAAAGGAACTTGCACATTTCGCAAAAAGCATTTCACTT
GAACCTCTGCAGAAAGAACTGATTCCTCAGCAGCGTCATGAAAGCAAACCAGTTAATGTTGATGAAGCTA
CAAGATTAATGGCTCTGTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218877 representing NM_001099671
Red=Cloning site Green=Tags(s)

MAKNKLRGPKSRNVFHIASQKNFKAKNKAKPVTTNLKKINIMNEEKVNRVNKAFVNVQKELAHFAKSISL
EPLQKELIPQQRHESKPVNVDEATRLMALL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001099671
ORF Size 300 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001099671.2, NP_001093141.1
RefSeq Size 1047 bp
RefSeq ORF 303 bp
Locus ID 401466
UniProt ID Q8N0T1, A0A024R838
Cytogenetics 8q21.2
MW 11.3 kDa
Summary Trans-acting factor in ribosome biogenesis required for efficient 40S and 60S subunit production.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001099671" in other vectors (4)
SKU Description Size Price
RC218877L3 Lenti ORF clone of Human chromosome 8 open reading frame 59 (C8orf59), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RC218877L4 Lenti ORF clone of Human chromosome 8 open reading frame 59 (C8orf59), transcript variant 2, mGFP tagged 10 ug
$450.00
RG218877 C8orf59 (tGFP-tagged) - Human chromosome 8 open reading frame 59 (C8orf59), transcript variant 2 10 ug
$350.00
SC316639 C8orf59 (untagged)-Human chromosome 8 open reading frame 59 (C8orf59), transcript variant 2 10 ug
$165.00

Plasmid Map for RC218877

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Cloning scheme for RC218877

Circular map for RC218877

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