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RTKN (NM_001015055) Human Tagged ORF Clone

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SKU
RC218810
RTKN (Myc-DDK-tagged)-Human rhotekin (RTKN), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $524.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RTKN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218810 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCTCCCGAAACCACCGGAGCCGGGTCACCGTGGCCAGGGGCTCCGCCCTGGAGATGGAGTTCAAAC
GCGGCCGCTTCCGACTCAGCCTCTTCAGCGACCTGCCCGAGGACACGGAGTTGCAGAGGAAGCTAGACCA
TGAGATCCGGATGAGGGAAGGGGCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCGAGAGCAGGCTCTGGAG
GCCACCAAGAGCCTGCTAGTGTGCAACAGCCGCATCCTCAGCTACATGGGCGAGCTGCAGCGGCGCAAGG
AGGCGCAGGTGCTGGGGAAGACAAGCCGGCGGCCTTCTGACAGTGGCCCGCCCGCTGAGCGCTCCCCCTG
CCGCGGCCGGGTCTGCATCTCTGACCTCCGGATTCCACTCATGTGGAAGGACACAGAATATTTCAAGAAC
AAAGGTGACTTGCACCGCTGGGCTGTGTTCCTGCTGCTGCAGCTGGGGGAACACATCCAGGACACAGAGA
TGATCCTAGTGGACAGGACCCTCACAGACATCTCCTTTCAGAGCAATGTGCTCTTCGCTGAGGCGGGGCC
AGACTTTGAACTGCGGTTAGAGCTGTATGGGGCCTGTGTGGAAGAAGAGGGGGCCCTGACTGGCGGCCCC
AAGAGGCTTGCCACCAAACTCAGCAGCTCCCTGGGCCGCTCCTCAGGGAGGCGTGTCCGGGCATCGCTGG
ACAGTGCTGGGGGTTCAGGGAGCAGTCCCATCTTGCTCCCCACCCCAGTTGTTGGTGGTCCTCGTTACCA
CCTCTTGGCTCACACCACACTCACCCTGGCAGCAGTGCAAGATGGATTCCGCACACATGACCTCACCCTT
GCCAGTCATGAGGAGAACCCTGCCTGGCTGCCCCTTTATGGTAGCGTGTGTTGCCGTCTGGCAGCTCAGC
CTCTCTGCATGACTCAGCCCACTGCAAGTGGTACCCTCAGGGTGCAGCAAGCTGGGGAGATGCAGAACTG
GGCACAAGTGCATGGAGTTCTGAAAGGCACAAACCTCTTCTGTTACCGGCAACCTGAGGATGCAGACACT
GGGGAAGAGCCGCTGCTTACTATTGCTGTCAACAAGGAGACTCGAGTCCGGGCAGGGGAGCTGGACCAGG
CTCTAGGACGGCCCTTCACCCTAAGCATCAGTAACCAGTATGGGGATGATGAGGTGACACACACCCTTCA
GACAGAAAGTCGGGAAGCACTGCAGAGCTGGATGGAGGCTCTGTGGCAGCTTTTCTTTGACATGAGCCAA
TGGAAGCAGTGCTGTGATGAAATCATGAAAATTGAAACTCCTGCTCCCCGGAAACCACCCCAAGCACTGG
CAAAGCAGGGGTCCTTGTACCATGAGATGGCTATTGAGCCGCTGGATGACATCGCAGCGGTGACAGACAT
CCTGACCCAGCGGGAGGGCGCAAGGCTGGAGACACCCCCACCCTGGCTGGCAATGTTTACAGACCAGCCT
GCCCTGCCTAACCCCTGCTCGCCTGCCTCAGTGGCCCCAGCCCCAGACTGGACCCACCCCCTGCCCTGGG
GGAGACCCCGAACCTTTTCCCTGGATGCTGTCCCCCCAGACCACTCCCCTAGGGCTCGCTCGGTTGCCCC
CCTCCCACCTCAGCGATCCCCACGGACCAGAGGCCTCTGCAGCAAAGGCCAACCTCGCACTTGGCTCCAG
TCACCAGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218810 protein sequence
Red=Cloning site Green=Tags(s)

MFSRNHRSRVTVARGSALEMEFKRGRFRLSLFSDLPEDTELQRKLDHEIRMREGACKLLAACSQREQALE
ATKSLLVCNSRILSYMGELQRRKEAQVLGKTSRRPSDSGPPAERSPCRGRVCISDLRIPLMWKDTEYFKN
KGDLHRWAVFLLLQLGEHIQDTEMILVDRTLTDISFQSNVLFAEAGPDFELRLELYGACVEEEGALTGGP
KRLATKLSSSLGRSSGRRVRASLDSAGGSGSSPILLPTPVVGGPRYHLLAHTTLTLAAVQDGFRTHDLTL
ASHEENPAWLPLYGSVCCRLAAQPLCMTQPTASGTLRVQQAGEMQNWAQVHGVLKGTNLFCYRQPEDADT
GEEPLLTIAVNKETRVRAGELDQALGRPFTLSISNQYGDDEVTHTLQTESREALQSWMEALWQLFFDMSQ
WKQCCDEIMKIETPAPRKPPQALAKQGSLYHEMAIEPLDDIAAVTDILTQREGARLETPPPWLAMFTDQP
ALPNPCSPASVAPAPDWTHPLPWGRPRTFSLDAVPPDHSPRARSVAPLPPQRSPRTRGLCSKGQPRTWLQ
SPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001015055
ORF Size 1689 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001015055.2
RefSeq Size 2208 bp
RefSeq ORF 1692 bp
Locus ID 6242
UniProt ID Q9BST9
Cytogenetics 2p13.1
MW 62.7 kDa
Summary This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001015055" in other vectors (6)
SKU Description Size Price
RC218810L1 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, Myc-DDK-tagged 10 ug
$824.00
RC218810L2 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, mGFP tagged 10 ug
$824.00
RC218810L3 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, Myc-DDK-tagged 10 ug
$824.00
RC218810L4 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, mGFP tagged 10 ug
$824.00
RG218810 RTKN (tGFP-tagged) - Human rhotekin (RTKN), transcript variant 1 10 ug
$724.00
SC301995 RTKN (untagged)-Human rhotekin (RTKN), transcript variant 1 10 ug
$577.00

Plasmid Map for RC218810

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218810
Circular map for RC218810

file-alt Citations

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