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RTKN (NM_001015055) Human Untagged Clone

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SKU
SC301995
RTKN (untagged)-Human rhotekin (RTKN), transcript variant 1
  $577.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RTKN
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC301995 representing NM_001015055.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTTCTCCCGAAACCACCGGAGCCGGGTCACCGTGGCCAGGGGCTCCGCCCTGGAGATGGAGTTCAAA
CGCGGCCGCTTCCGACTCAGCCTCTTCAGCGACCTGCCCGAGGACACGGAGTTGCAGAGGAAGCTAGAC
CATGAGATCCGGATGAGGGAAGGGGCCTGTAAGCTGCTGGCAGCCTGCTCCCAGCGAGAGCAGGCTCTG
GAGGCCACCAAGAGCCTGCTAGTGTGCAACAGCCGCATCCTCAGCTACATGGGCGAGCTGCAGCGGCGC
AAGGAGGCGCAGGTGCTGGGGAAGACAAGCCGGCGGCCTTCTGACAGTGGCCCGCCCGCTGAGCGCTCC
CCCTGCCGCGGCCGGGTCTGCATCTCTGACCTCCGGATTCCACTCATGTGGAAGGACACAGAATATTTC
AAGAACAAAGGTGACTTGCACCGCTGGGCTGTGTTCCTGCTGCTGCAGCTGGGGGAACACATCCAGGAC
ACAGAGATGATCCTAGTGGACAGGACCCTCACAGACATCTCCTTTCAGAGCAATGTGCTCTTCGCTGAG
GCGGGGCCAGACTTTGAACTGCGGTTAGAGCTGTATGGGGCCTGTGTGGAAGAAGAGGGGGCCCTGACT
GGCGGCCCCAAGAGGCTTGCCACCAAACTCAGCAGCTCCCTGGGCCGCTCCTCAGGGAGGCGTGTCCGG
GCATCGCTGGACAGTGCTGGGGGTTCAGGGAGCAGTCCCATCTTGCTCCCCACCCCAGTTGTTGGTGGT
CCTCGTTACCACCTCTTGGCTCACACCACACTCACCCTGGCAGCAGTGCAAGATGGATTCCGCACACAT
GACCTCACCCTTGCCAGTCATGAGGAGAACCCTGCCTGGCTGCCCCTTTATGGTAGCGTGTGTTGCCGT
CTGGCAGCTCAGCCTCTCTGCATGACTCAGCCCACTGCAAGTGGTACCCTCAGGGTGCAGCAAGCTGGG
GAGATGCAGAACTGGGCACAAGTGCATGGAGTTCTGAAAGGCACAAACCTCTTCTGTTACCGGCAACCT
GAGGATGCAGACACTGGGGAAGAGCCGCTGCTTACTATTGCTGTCAACAAGGAGACTCGAGTCCGGGCA
GGGGAGCTGGACCAGGCTCTAGGACGGCCCTTCACCCTAAGCATCAGTAACCAGTATGGGGATGATGAG
GTGACACACACCCTTCAGACAGAAAGTCGGGAAGCACTGCAGAGCTGGATGGAGGCTCTGTGGCAGCTT
TTCTTTGACATGAGCCAATGGAAGCAGTGCTGTGATGAAATCATGAAAATTGAAACTCCTGCTCCCCGG
AAACCACCCCAAGCACTGGCAAAGCAGGGGTCCTTGTACCATGAGATGGCTATTGAGCCGCTGGATGAC
ATCGCAGCGGTGACAGACATCCTGACCCAGCGGGAGGGCGCAAGGCTGGAGACACCCCCACCCTGGCTG
GCAATGTTTACAGACCAGCCTGCCCTGCCTAACCCCTGCTCGCCTGCCTCAGTGGCCCCAGCCCCAGAC
TGGACCCACCCCCTGCCCTGGGGGAGACCCCGAACCTTTTCCCTGGATGCTGTCCCCCCAGACCACTCC
CCTAGGGCTCGCTCGGTTGCCCCCCTCCCACCTCAGCGATCCCCACGGACCAGAGGCCTCTGCAGCAAA
GGCCAACCTCGCACTTGGCTCCAGTCACCAGTGTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001015055
Insert Size 1692 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001015055.1
RefSeq Size 2208 bp
RefSeq ORF 1692 bp
Locus ID 6242
UniProt ID Q9BST9
Cytogenetics 2p13.1
MW 62.7 kDa
Summary This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) encodes the longest isoform (a).
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Documents
Resources
"NM_001015055" in other vectors (6)
SKU Description Size Price
RC218810 RTKN (Myc-DDK-tagged)-Human rhotekin (RTKN), transcript variant 1 10 ug
$524.00
RC218810L1 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, Myc-DDK-tagged 10 ug
$824.00
RC218810L2 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, mGFP tagged 10 ug
$824.00
RC218810L3 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, Myc-DDK-tagged 10 ug
$824.00
RC218810L4 Lenti ORF clone of Human rhotekin (RTKN), transcript variant 1, mGFP tagged 10 ug
$824.00
RG218810 RTKN (tGFP-tagged) - Human rhotekin (RTKN), transcript variant 1 10 ug
$724.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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