PROP1 (NM_006261) Human Tagged ORF Clone

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SKU
RC218746
PROP1 (Myc-DDK-tagged)-Human PROP paired-like homeobox 1 (PROP1)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PROP1
Synonyms CPHD2; PROP-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218746 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGCAGAAAGGAGGCGCCAGGCTGAGAAGCCAAAGAAGGGGCGAGTCGGCAGCAGCCTGTTGCCTG
AGAGACACCCGGCCACTGGGACCCCGACCACCACGGTGGACTCGAGTGCTCCACCCTGCAGAAGGCTCCC
TGGTGCAGGAGGGGGGAGATCAAGGTTCTCCCCGCAAGGAGGACAGAGGGGCCGCCCGCACTCCCGGCGC
CGCCACCGCACCACCTTCAGCCCAGTGCAGTTGGAACAGCTGGAGTCAGCCTTTGGGAGGAACCAGTACC
CCGACATCTGGGCCCGAGAGAGTCTTGCCCGGGACACTGGCCTCAGTGAGGCCCGAATCCAGGTCTGGTT
CCAGAACCGCAGAGCTAAGCAACGGAAGCAAGAGCGCTCACTGCTTCAGCCTCTGGCCCATCTGTCTCCT
GCCGCCTTTTCCAGCTTCTTGCCAGAGTCCACTGCTTGCCCCTATTCTTACGCAGCACCACCACCACCAG
TGACCTGCTTCCCTCACCCCTACAGCCATGCCCTCCCTTCCCAGCCCTCCACAGGAGGCGCCTTTGCTTT
GTCACACCAGTCTGAGGACTGGTACCCTACCTTGCACCCAGCCCCTGCCGGCCATCTGCCCTGCCCCCCA
CCCCCTCCCATGCTCCCCCTCAGCCTTGAGCCATCCAAGTCCTGGAAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218746 protein sequence
Red=Cloning site Green=Tags(s)

MEAERRRQAEKPKKGRVGSSLLPERHPATGTPTTTVDSSAPPCRRLPGAGGGRSRFSPQGGQRGRPHSRR
RHRTTFSPVQLEQLESAFGRNQYPDIWARESLARDTGLSEARIQVWFQNRRAKQRKQERSLLQPLAHLSP
AAFSSFLPESTACPYSYAAPPPPVTCFPHPYSHALPSQPSTGGAFALSHQSEDWYPTLHPAPAGHLPCPP
PPPMLPLSLEPSKSWN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006261
ORF Size 678 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006261.4, NP_006252.3
RefSeq Size 1464 bp
RefSeq ORF 681 bp
Locus ID 5626
UniProt ID O75360, A0A0G2JQ02
Cytogenetics 5q35.3
Protein Families Druggable Genome, Transcription Factors
MW 25 kDa
Summary This gene encodes a paired-like homeodomain transcription factor in the developing pituitary gland. Expression occurs prior to and is required for expression of pou domain transcription factor 1, which is responsible for pituitary development and hormone expression. Mutations in this gene have been associated with combined pituitary hormone deficiency-2 as well as deficiencies in luteinizing hormone, follicle-stimulating hormone, growth hormone, prolactin, and thyroid-stimulating hormone. provided by RefSeq, Sep 2011
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Documents
Resources
"NM_006261" in other vectors (4)
SKU Description Size Price
RC218746L3 Lenti ORF clone of Human PROP paired-like homeobox 1 (PROP1), Myc-DDK-tagged 10 ug
$750.00
RC218746L4 Lenti ORF clone of Human PROP paired-like homeobox 1 (PROP1), mGFP tagged 10 ug
$750.00
RG218746 PROP1 (tGFP-tagged) - Human PROP paired-like homeobox 1 (PROP1) 10 ug
$650.00
SC303762 PROP1 (untagged)-Human PROP paired-like homeobox 1 (PROP1) 10 ug
$480.00

Plasmid Map for RC218746

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Cloning scheme for RC218746

Circular map for RC218746

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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