BSPRY (NM_017688) Human Tagged ORF Clone

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SKU
RC218642
BSPRY (Myc-DDK-tagged)-Human B-box and SPRY domain containing (BSPRY)
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BSPRY
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218642 representing NM_017688
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCCGAGGGCGCGGAGCCGGGGCCGGGGTCCGGGTCCGGGCCCGGGCCGGGGCCACTCTGCCCCG
AACACGGCCAGGCTCTGAGCTGGTTCTGCGGCTCCGAGCGACGGCCCGTGTGCGCCGCCTGCGCGGGGTT
GGGCGGTCGCTGCCGGGGGCACCGCATCCGCCGGGCGGAGGAGCGCGCCGAGGAGCTGCGGAACAAGATT
GTGGACCAGTGTGAGAGGCTGCAGTTACAGAGTGCTGCCATCACCAAGTATGTGGCGGACGTCCTGCCGG
GGAAGAATCAAAGAGCAGTGAGCATGGCCAGTGCAGCGAGGGAACTGGTTATCCAGCGGTTGAGTCTGGT
GAGGAGTCTTTGCGAGAGCGAGGAGCAGCGGTTACTGGAACAGGTGCATGGCGAAGAGGAGCGGGCCCAC
CAGAGCATCCTGACACAGCGGGTGCACTGGGCCGAGGCGCTGCAGAAACTTGACACCATCCGCACTGGCC
TGGTGGGCATGCTTACTCACCTGGATGACCTCCAGCTGATTCAGAAGGAGCAAGAGATTTTCGAGAGGAC
CGAAGAAGCAGAGGGCATTTTGGATCCCCAGGAGTCGGAAATGTTAAACTTTAATGAGAAGTGCACTCGG
AGCCCACTACTGACCCAACTCTGGGCAACGGCGGTTCTTGGGTCTCTCTCAGGCACAGAGGACATACGGA
TCGATGAGAGGACAGTCAGCCCCTTCCTGCAATTGTCAGATGATCGAAAGACCCTGACCTTCAGCACCAA
GAAGTCAAAGGCCTGTGCAGATGGCCCGGAGCGCTTCGACCACTGGCCCAATGCCCTGGCTGCCACCTCC
TTCCAGAATGGGCTCCATGCCTGGATGGTGAATGTCCAGAACAGTTGTGCCTATAAGGTGGGCGTGGCTT
CAGGCCACCTGCCCCGCAAGGGTTCTGGCAGTGACTGCCGTCTGGGCCACAATGCCTTCTCCTGGGTCTT
CTCTCGCTATGATCAGGAGTTTCGTTTCTCACACAATGGGCAGCACGAGCCCCTGGGGCTGCTGCGGGGC
CCAGCCCAGCTGGGTGTAGTGCTGGACTTGCAGGTTCAGGAGCTGCTCTTCTATGAGCCAGCCTCCGGCA
CAGTGCTCTGTGCCCATCATGTGTCCTTCCCGGGGCCCCTCTTCCCAGTCTTTGCTGTGGCCGATCAGAC
CATTTCTATCGTCCGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218642 representing NM_017688
Red=Cloning site Green=Tags(s)

MSAEGAEPGPGSGSGPGPGPLCPEHGQALSWFCGSERRPVCAACAGLGGRCRGHRIRRAEERAEELRNKI
VDQCERLQLQSAAITKYVADVLPGKNQRAVSMASAARELVIQRLSLVRSLCESEEQRLLEQVHGEEERAH
QSILTQRVHWAEALQKLDTIRTGLVGMLTHLDDLQLIQKEQEIFERTEEAEGILDPQESEMLNFNEKCTR
SPLLTQLWATAVLGSLSGTEDIRIDERTVSPFLQLSDDRKTLTFSTKKSKACADGPERFDHWPNALAATS
FQNGLHAWMVNVQNSCAYKVGVASGHLPRKGSGSDCRLGHNAFSWVFSRYDQEFRFSHNGQHEPLGLLRG
PAQLGVVLDLQVQELLFYEPASGTVLCAHHVSFPGPLFPVFAVADQTISIVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017688
ORF Size 1206 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017688.3
RefSeq Size 2354 bp
RefSeq ORF 1209 bp
Locus ID 54836
UniProt ID Q5W0U4
Cytogenetics 9q32
Domains PRY, SPRY
Protein Families Druggable Genome
MW 44.2 kDa
Summary May regulate epithelial calcium transport by inhibiting TRPV5 activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_017688" in other vectors (7)
SKU Description Size Price
RC218642L1 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), Myc-DDK-tagged 10 ug
$757.00
RC218642L2 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), mGFP tagged 10 ug
$757.00
RC218642L3 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), Myc-DDK-tagged 10 ug
$757.00
RC218642L4 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), mGFP tagged 10 ug
$757.00
RG218642 BSPRY (tGFP-tagged) - Human B-box and SPRY domain containing (BSPRY) 10 ug
$657.00
SC114049 BSPRY (untagged)-Human B-box and SPRY domain containing (BSPRY) 10 ug
$300.00
SC317637 BSPRY (untagged)-Human B-box and SPRY domain containing (BSPRY) 10 ug
$503.00

Plasmid Map for RC218642

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Cloning scheme for RC218642

Circular map for RC218642

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