BSPRY (NM_017688) Human Untagged Clone

SKU
SC317637
BSPRY (untagged)-Human B-box and SPRY domain containing (BSPRY)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol BSPRY
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC317637 representing NM_017688.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGTCCGCCGAGGGCGCGGAGCCGGGGCCGGGGTCCGGGTCCGGGCCCGGGCCGGGGCCACTCTGCCCC
GAACACGGCCAGGCTCTGAGCTGGTTCTGCGGCTCCGAGCGACGGCCCGTGTGCGCCGCCTGCGCGGGG
TTGGGCGGTCGCTGCCGGGGGCACCGCATCCGCCGGGCGGAGGAGCGCGCCGAGGAGCTGCGGAACAAG
ATTGTGGACCAGTGTGAGAGGCTGCAGTTACAGAGTGCTGCCATCACCAAGTATGTGGCGGACGTCCTG
CCGGGGAAGAATCAAAGAGCAGTGAGCATGGCCAGTGCAGCGAGGGAACTGGTTATCCAGCGGTTGAGT
CTGGTGAGGAGTCTTTGCGAGAGCGAGGAGCAGCGGTTACTGGAACAGGTGCATGGCGAAGAGGAGCGG
GCCCACCAGAGCATCCTGACACAGCGGGTGCACTGGGCCGAGGCGCTGCAGAAACTTGACACCATCCGC
ACTGGCCTGGTGGGCATGCTTACTCACCTGGATGACCTCCAGCTGATTCAGAAGGAGCAAGAGATTTTC
GAGAGGACCGAAGAAGCAGAGGGCATTTTGGATCCCCAGGAGTCGGAAATGTTAAACTTTAATGAGAAG
TGCACTCGGAGCCCACTACTGACCCAACTCTGGGCAACGGCGGTTCTTGGGTCTCTCTCAGGCACAGAG
GACATACGGATCGATGAGAGGACAGTCAGCCCCTTCCTGCAATTGTCAGATGATCGAAAGACCCTGACC
TTCAGCACCAAGAAGTCAAAGGCCTGTGCAGATGGCCCGGAGCGCTTCGACCACTGGCCCAATGCCCTG
GCTGCCACCTCCTTCCAGAATGGGCTCCATGCCTGGATGGTGAATGTCCAGAACAGTTGTGCCTATAAG
GTGGGCGTGGCTTCAGGCCACCTGCCCCGCAAGGGTTCTGGCAGTGACTGCCGTCTGGGCCACAATGCC
TTCTCCTGGGTCTTCTCTCGCTATGATCAGGAGTTTCGTTTCTCACACAATGGGCAGCACGAGCCCCTG
GGGCTGCTGCGGGGCCCAGCCCAGCTGGGTGTAGTGCTGGACTTGCAGGTTCAGGAGCTGCTCTTCTAT
GAGCCAGCCTCCGGCACAGTGCTCTGTGCCCATCATGTGTCCTTCCCGGGGCCCCTCTTCCCAGTCTTT
GCTGTGGCCGATCAGACCATTTCTATCGTCCGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_017688
Insert Size 1209 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017688.2
RefSeq Size 2354 bp
RefSeq ORF 1209 bp
Locus ID 54836
UniProt ID Q5W0U4
Cytogenetics 9q32
Domains PRY, SPRY
Protein Families Druggable Genome
MW 44.4 kDa
Summary May regulate epithelial calcium transport by inhibiting TRPV5 activity.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1.
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Documents
Resources
"NM_017688" in other vectors (7)
SKU Description Size Price
RC218642 BSPRY (Myc-DDK-tagged)-Human B-box and SPRY domain containing (BSPRY) 10 ug
$457.00
RC218642L1 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), Myc-DDK-tagged 10 ug
$757.00
RC218642L2 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), mGFP tagged 10 ug
$757.00
RC218642L3 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), Myc-DDK-tagged 10 ug
$757.00
RC218642L4 Lenti ORF clone of Human B-box and SPRY domain containing (BSPRY), mGFP tagged 10 ug
$757.00
RG218642 BSPRY (tGFP-tagged) - Human B-box and SPRY domain containing (BSPRY) 10 ug
$657.00
SC114049 BSPRY (untagged)-Human B-box and SPRY domain containing (BSPRY) 10 ug
$300.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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