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BCO2 (NM_031938) Human Tagged ORF Clone

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SKU
RC218635
BCO2 (Myc-DDK-tagged)-Human beta-carotene oxygenase 2 (BCO2), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $539.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BCO2
Synonyms B-DIOX-II; BCDO2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218635 representing NM_031938
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTTTTCGAGTCTTTCTCCATTTTATCAGGAGTCATTCTGCCACTGCAGTGGATTTCCTTCCTGTGA
TGGTGCACCGGCTCCCAGTTTTCAAAAGGTACATGGGAAATACTCCTCAGAAAAAAGCCGTCTTTGGGCA
GTGTCGGGGTCTGCCATGTGTTGCACCGCTGCTGACCACAGTGGAAGAGGCTCCACGGGGCATCTCTGCT
CGAGTCTGGGGACATTTTCCTAAGTGGCTCAATGGCTCTCTACTTCGAATTGGACCTGGGAAATTCGAGT
TTGGGAAGGATAAGTACAATCATTGGTTTGATGGGATGGCGCTGCTTCACCAGTTCAGAATGGCAAAGGG
CACAGTGACATACAGGAGCAAGTTTCTACAGAGTGATACATATAAGGCCAACAGTGCTAAAAACCGAATT
GTGATCTCAGAATTTGGCACACTGGCTCTCCCGGATCCATGCAAGAATGTTTTTGAACGTTTCATGTCCA
GGTTTGAGCTGCCTGGTAAAGCTGCAGCCATGACTGACAATACTAATGTCAACTATGTGCGGTACAAGGG
TGATTACTACCTCTGCACTGAGACCAACTTTATGAATAAAGTGGACATTGAAACTCTGGAAAAAACAGAA
AAGGTAGATTGGAGCAAATTTATTGCTGTGAATGGAGCAACTGCACATCCTCATTATGACCTGGATGGAA
CAGCATACAATATGGGGAACTCCTTTGGGCCATATGGTTTCTCCTATAAGGTTATTCGGGTTCCTCCAGA
GAAGGTGGACCTTGGGGAGACAATCCATGGAGTCCAGGTGATATGTTCTATTGCTTCTACAGAGAAAGGG
AAACCTTCTTACTACCATAGCTTTGGAATGACAAGGAACTATATAATTTTCATTGAACAACCTCTAAAGA
TGAACCTGTGGAAAATTGCCACTTCTAAAATTCGGGGAAAGGCCTTTTCAGATGGGATAAGCTGGGAACC
CCAGTGTAATACGCGGTTTCATGTGGTGGAAAAACGCACTGGACAGCTCCTTCCAGGGAGATACTACAGC
AAACCTTTTGTTACATTTCATCAAATCAATGCCTTTGAGGACCAGGGCTGTGTTATAATTGATTTGTGCT
GTCAAGATAATGGAAGAACCCTAGAAGTTTACCAGTTACAGAATCTCAGGAAGGCTGGGGAAGGGCTTGA
TCAGGTCCATAATTCAGCAGCCAAATCTTTCCCTCGAAGGTTTGTTTTGCCTTTAAATGTCAGTTTGAAT
GCCCCTGAGGGAGACAACCTGAGTCCATTGTCCTATACTTCAGCCAGTGCTGTGAAACAGGCTGATGGAA
CGATCTGGTGCTCTCATGAAAATCTACATCAGGAGGACCTAGAAAAGGAAGGAGGCATTGAATTTCCTCA
GATCTACTATGATCGATTCAGTGGCAAAAAGTATCATTTCTTTTATGGCTGTGGCTTTCGGCATTTAGTG
GGGGATTCTCTGATCAAGGTTGATGTGGTGAATAAGACACTGAAGGTTTGGAGAGAAGATGGCTTTTATC
CCTCAGAACCTGTTTTTGTTCCAGCACCAGGAACCAATGAAGAAGATGGTGGGGTTATTCTTTCTGTGGT
GATCACTCCCAACCAGAATGAAAGCAATTTTATCCTAGTTTTGGATGCCAAGAACTTTGAAGAGCTGGGC
CGAGCAGAGGTACCTGTGCAGATGCCTTATGGGTTCCATGGTACCTTCATACCCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218635 representing NM_031938
Red=Cloning site Green=Tags(s)

MFFRVFLHFIRSHSATAVDFLPVMVHRLPVFKRYMGNTPQKKAVFGQCRGLPCVAPLLTTVEEAPRGISA
RVWGHFPKWLNGSLLRIGPGKFEFGKDKYNHWFDGMALLHQFRMAKGTVTYRSKFLQSDTYKANSAKNRI
VISEFGTLALPDPCKNVFERFMSRFELPGKAAAMTDNTNVNYVRYKGDYYLCTETNFMNKVDIETLEKTE
KVDWSKFIAVNGATAHPHYDLDGTAYNMGNSFGPYGFSYKVIRVPPEKVDLGETIHGVQVICSIASTEKG
KPSYYHSFGMTRNYIIFIEQPLKMNLWKIATSKIRGKAFSDGISWEPQCNTRFHVVEKRTGQLLPGRYYS
KPFVTFHQINAFEDQGCVIIDLCCQDNGRTLEVYQLQNLRKAGEGLDQVHNSAAKSFPRRFVLPLNVSLN
APEGDNLSPLSYTSASAVKQADGTIWCSHENLHQEDLEKEGGIEFPQIYYDRFSGKKYHFFYGCGFRHLV
GDSLIKVDVVNKTLKVWREDGFYPSEPVFVPAPGTNEEDGGVILSVVITPNQNESNFILVLDAKNFEELG
RAEVPVQMPYGFHGTFIPI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031938
ORF Size 1737 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031938.7
RefSeq Size 2900 bp
RefSeq ORF 1740 bp
Locus ID 83875
UniProt ID Q9BYV7, B2RCV8
Cytogenetics 11q23.1
MW 65.5 kDa
Summary This gene encodes an enzyme which oxidizes carotenoids such as beta-carotene during the biosynthesis of vitamin A. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2012
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Documents
Resources
"NM_031938" in other vectors (6)
SKU Description Size Price
RC218635L1 Lenti ORF clone of Human beta-carotene oxygenase 2 (BCO2), transcript variant 1, Myc-DDK-tagged 10 ug
$839.00
RC218635L2 Lenti ORF clone of Human beta-carotene oxygenase 2 (BCO2), transcript variant 1, mGFP tagged 10 ug
$839.00
RC218635L3 Lenti ORF clone of Human beta-carotene oxygenase 2 (BCO2), transcript variant 1, Myc-DDK-tagged 10 ug
$839.00
RC218635L4 Lenti ORF clone of Human beta-carotene oxygenase 2 (BCO2), transcript variant 1, mGFP tagged 10 ug
$839.00
RG218635 BCO2 (tGFP-tagged) - Human beta-carotene oxygenase 2 (BCO2), transcript variant 1 10 ug
$739.00
SC305371 BCO2 (untagged)-Human beta-carotene oxygenase 2 (BCO2), transcript variant 1 10 ug
$594.00

Plasmid Map for RC218635

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC218635
Circular map for RC218635

file-alt Citations

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