GSC2 (NM_005315) Human Tagged ORF Clone

  • TrueORF®
SKU
RC218631
GSC2 (Myc-DDK-tagged)-Human goosecoid homeobox 2 (GSC2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GSC2
Synonyms GSCL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218631 representing NM_005315
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCAGCGGCTGGGGGCGCGGCGAGCCGCCGGGGTGCCGGGCGGCCCTGCCCCTTCTCCATCGAGC
ACATCCTCTCCAGCCTGCCCGAGCGGAGCCTCCCGGCCCGGGCCGCCTGCCCACCGCAGCCCGCCGGTCG
CCAGAGCCCCGCGAAGCCAGAGGAGCCCGGGGCGCCCGAGGCTGCGCCCTGCGCCTGCTGCTGCTGCTGC
GGCCCCCGCGCGGCGCCCTGCGGGCCCCCAGAGGCGGCCGCCGGGCTGGGCGCTCGTCTGGCGTGGCCGC
TGAGGCTGGGACCGGCGGTGCCCTTGTCTCTGGGTGCGCCAGCCGGAGGTTCCGGGGCGCTCCCGGGCGC
GGTCGGCCCGGGTTCGCAGCGGCGCACGAGGCGCCACCGCACCATCTTCAGCGAAGAGCAGCTGCAGGCG
CTCGAGGCGCTTTTCGTGCAGAACCAGTATCCTGACGTGAGTACGCGCGAGCGCCTGGCCGGCCGCATCC
GCCTTCGCGAGGAGCGCGTGGAGGTCTGGTTCAAGAACCGCCGGGCCAAATGGCGACACCAGAAGCGCGC
GTCGGCTTCCGCGAGGCTCCTGCCCGGCGTCAAGAAGTCCCCGAAGGGGAGCTGC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218631 representing NM_005315
Red=Cloning site Green=Tags(s)

MAAAAGGAASRRGAGRPCPFSIEHILSSLPERSLPARAACPPQPAGRQSPAKPEEPGAPEAAPCACCCCC
GPRAAPCGPPEAAAGLGARLAWPLRLGPAVPLSLGAPAGGSGALPGAVGPGSQRRTRRHRTIFSEEQLQA
LEALFVQNQYPDVSTRERLAGRIRLREERVEVWFKNRRAKWRHQKRASASARLLPGVKKSPKGSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005315
ORF Size 615 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005315.2
RefSeq Size 618 bp
RefSeq ORF 618 bp
Locus ID 2928
UniProt ID O15499
Cytogenetics 22q11.21
MW 21.4 kDa
Summary Goosecoidlike (GSCL), a homeodomain-containing gene, resides in the critical region for VCFS/DGS on 22q11. Velocardiofacial syndrome (VCFS) is a developmental disorder characterized by conotruncal heart defects, craniofacial anomalies, and learning disabilities. VCFS is phenotypically related to DiGeorge syndrome (DGS) and both syndromes are associated with hemizygous 22q11 deletions. Because many of the tissues and structures affected in VCFS/DGS derive from the pharyngeal arches of the developing embryo, it is believed that haploinsufficiency of a gene involved in embryonic development may be responsible for its etiology. The gene is expressed in a limited number of adult tissues, as well as in early human development. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005315" in other vectors (4)
SKU Description Size Price
RC218631L3 Lenti ORF clone of Human goosecoid homeobox 2 (GSC2), Myc-DDK-tagged 10 ug
$600.00
RC218631L4 Lenti ORF clone of Human goosecoid homeobox 2 (GSC2), mGFP tagged 10 ug
$600.00
RG218631 GSC2 (tGFP-tagged) - Human goosecoid homeobox 2 (GSC2) 10 ug
$500.00
SC303614 GSC2 (untagged)-Human goosecoid homeobox 2 (GSC2) 10 ug
$330.00

Plasmid Map for RC218631

Download sequence for

Cloning scheme for RC218631

Circular map for RC218631

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