GSC2 (NM_005315) Human Untagged Clone

SKU
SC303614
GSC2 (untagged)-Human goosecoid homeobox 2 (GSC2)
  $330.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GSC2
Synonyms GSCL
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC303614 representing NM_005315.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGGCAGCGGCTGGGGGCGCGGCGAGCCGCCGGGGTGCCGGGCGGCCCTGCCCCTTCTCCATCGAG
CACATCCTCTCCAGCCTGCCCGAGCGGAGCCTCCCGGCCCGGGCCGCCTGCCCACCGCAGCCCGCCGGT
CGCCAGAGCCCCGCGAAGCCAGAGGAGCCCGGGGCGCCCGAGGCTGCGCCCTGCGCCTGCTGCTGCTGC
TGCGGCCCCCGCGCGGCGCCCTGCGGGCCCCCAGAGGCGGCCGCCGGGCTGGGCGCTCGTCTGGCGTGG
CCGCTGAGGCTGGGACCGGCGGTGCCCTTGTCTCTGGGTGCGCCAGCCGGAGGTTCCGGGGCGCTCCCG
GGCGCGGTCGGCCCGGGTTCGCAGCGGCGCACGAGGCGCCACCGCACCATCTTCAGCGAAGAGCAGCTG
CAGGCGCTCGAGGCGCTTTTCGTGCAGAACCAGTATCCTGACGTGAGTACGCGCGAGCGCCTGGCCGGC
CGCATCCGCCTTCGCGAGGAGCGCGTGGAGGTCTGGTTCAAGAACCGCCGGGCCAAATGGCGACACCAG
AAGCGCGCGTCGGCTTCCGCGAGGCTCCTGCCCGGCGTCAAGAAGTCCCCGAAGGGGAGCTGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_005315
Insert Size 618 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005315.1
RefSeq Size 618 bp
RefSeq ORF 618 bp
Locus ID 2928
UniProt ID O15499
Cytogenetics 22q11.21
MW 21.5 kDa
Summary Goosecoidlike (GSCL), a homeodomain-containing gene, resides in the critical region for VCFS/DGS on 22q11. Velocardiofacial syndrome (VCFS) is a developmental disorder characterized by conotruncal heart defects, craniofacial anomalies, and learning disabilities. VCFS is phenotypically related to DiGeorge syndrome (DGS) and both syndromes are associated with hemizygous 22q11 deletions. Because many of the tissues and structures affected in VCFS/DGS derive from the pharyngeal arches of the developing embryo, it is believed that haploinsufficiency of a gene involved in embryonic development may be responsible for its etiology. The gene is expressed in a limited number of adult tissues, as well as in early human development. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_005315" in other vectors (4)
SKU Description Size Price
RC218631 GSC2 (Myc-DDK-tagged)-Human goosecoid homeobox 2 (GSC2) 10 ug
$289.00 $300.00
RC218631L3 Lenti ORF clone of Human goosecoid homeobox 2 (GSC2), Myc-DDK-tagged 10 ug
$600.00
RC218631L4 Lenti ORF clone of Human goosecoid homeobox 2 (GSC2), mGFP tagged 10 ug
$600.00
RG218631 GSC2 (tGFP-tagged) - Human goosecoid homeobox 2 (GSC2) 10 ug
$500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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