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SLIT1 (NM_003061) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC218562
SLIT1 (Myc-DDK-tagged)-Human slit homolog 1 (Drosophila) (SLIT1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$589.00 $1,312.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "SLIT1 or NM_003061"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SLIT1
Synonyms MEGF4; SLIL1; SLIT-1; SLIT3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218562 representing NM_003061
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGACTCCCGGGTGGGGGTCCTCGGCGGGGCCGGTCCGGCCGGAGCTCTGGCTGCTGCTGTGGG
CAGCCGCGTGGCGCCTGGGTGCCTCGGCGTGCCCCGCCCTCTGCACCTGCACCGGAACCACGGTGGACTG
CCACGGCACGGGGCTGCAGGCCATTCCCAAGAATATACCTCGGAACACCGAGCGCCTGGAACTCAATGGC
AACAACATCACTCGGATCCATAAGAATGACTTTGCGGGGCTCAAGCAGCTGCGGGTGCTGCAGCTGATGG
AGAACCAGATTGGAGCAGTGGAACGTGGTGCTTTTGATGACATGAAGGAGCTGGAGCGGCTGCGACTGAA
CCGAAACCAGCTGCACATGTTACCGGAACTGCTGTTCCAGAACAACCAGGCTTTGTCAAGACTGGACTTG
AGTGAGAACGCCATCCAGGCCATCCCCAGGAAAGCTTTTCGGGGAGCTACGGACCTTAAAAATTTACAGC
TGGACAAGAACCAGATCAGCTGCATTGAGGAAGGGGCCTTCCGTGCTCTGCGGGGGCTGGAGGTGCTGAC
CCTGAACAACAACAATATCACCACCATCCCCGTGTCCAGCTTCAACCATATGCCCAAGCTACGGACCTTC
CGCCTGCACTCCAACCACCTGTTTTGCGACTGCCACCTGGCCTGGCTCTCGCAGTGGCTGAGGCAGCGGC
CAACCATCGGGCTCTTCACCCAGTGCTCGGGCCCAGCCAGCCTGCGTGGCCTCAATGTGGCAGAGGTCCA
GAAGAGTGAGTTCAGCTGCTCAGGCCAGGGAGAAGCGGGGCGCGTGCCCACCTGCACCCTGTCCTCCGGC
TCCTGCCCGGCCATGTGCACCTGCAGCAATGGCATCGTGGACTGTCGTGGAAAAGGCCTCACTGCCATCC
CGGCCAACCTGCCCGAGACCATGACGGAGATACGCCTGGAGCTGAACGGCATCAAGTCCATCCCTCCTGG
AGCCTTCTCACCCTACAGAAAGCTACGGAGGATAGACCTGAGCAACAATCAGATCGCTGAGATTGCACCC
GACGCCTTCCAGGGCCTCCGCTCCCTGAACTCGCTGGTCCTCTATGGAAACAAGATCACAGACCTCCCCC
GTGGTGTGTTTGGAGGCCTATACACCCTACAGCTCCTGCTCCTGAATGCCAACAAGATCAACTGCATCCG
GCCCGATGCCTTCCAGGACCTGCAGAACCTCTCACTGCTCTCCCTGTATGACAACAAGATCCAGAGCCTC
GCCAAGGGCACTTTCACCTCCCTGCGGGCCATCCAGACTCTGCACCTGGCGCAGAACCCTTTCATTTGCG
ACTGTAACCTCAAGTGGCTGGCAGACTTCCTGCGCACCAATCCCATCGAGACGAGTGGTGCCCGCTGTGC
CAGTCCCCGGCGCCTCGCCAACAAGCGCATCGGGCAGATCAAGAGCAAGAAGTTCCGGTGCTCAGCCAAA
GAGCAGTACTTCATTCCAGGCACGGAGGATTACCAGCTGAACAGCGAGTGCAACAGCGACGTGGTCTGTC
CCCACAAGTGCCGCTGTGAGGCCAACGTGGTGGAGTGCTCCAGCCTGAAGCTCACCAAGATCCCTGAGCG
CATCCCCCAGTCCACGGCAGAACTGCGATTGAATAACAATGAGATTTCCATCCTGGAGGCCACTGGGATG
TTTAAAAAACTTACACATCTGAAGAAAATCAATCTGAGCAACAACAAGGTGTCAGAAATTGAAGATGGGG
CCTTCGAGGGCGCAGCCTCTGTGAGCGAGCTGCACCTAACTGCCAACCAGCTGGAGTCCATCCGGAGCGG
CATGTTCCGGGGTCTGGATGGCTTGAGGACCCTAATGCTGCGGAACAACCGCATCAGCTGCATCCACAAC
GACAGCTTCACGGGCCTGCGCAACGTCCGGCTCCTCTCGCTCTACGACAACCAGATCACCACCGTATCCC
CAGGAGCCTTCGACACCCTCCAGTCCCTCTCCACACTGAATCTCCTGGCCAACCCTTTCAACTGCAACTG
CCAGCTGGCCTGGCTAGGAGGCTGGCTACGGAAGCGCAAGATCGTGACGGGGAACCCGCGATGCCAGAAC
CCTGACTTTTTGCGGCAGATTCCCCTGCAGGACGTGGCCTTCCCTGATTTCAGGTGTGAGGAAGGCCAGG
AGGAGGGGGGCTGCCTGCCCCGCCCACAGTGCCCACAGGAGTGCGCCTGCCTGGACACCGTGGTCCGATG
CAGCAACAAGCACCTGCGGGCCCTGCCCAAGGGCATTCCCAAGAATGTCACAGAACTCTATTTGGACGGG
AACCAGTTCACGCTGGTTCCGGGACAGCTGTCTACCTTCAAGTACCTGCAGCTCGTGGACCTGAGCAACA
ACAAGATCAGTTCCTTAAGCAATTCCTCCTTCACCAACATGAGCCAGCTGACCACTCTGATCCTCAGCTA
CAATGCCCTGCAGTGCATCCCGCCTTTGGCCTTCCAGGGACTCCGCTCCCTGCGCCTGCTGTCTCTCCAC
GGCAATGACATCTCCACCCTCCAAGAGGGCATCTTTGCAGACGTGACCTCCCTGTCTCACCTGGCCATTG
GTGCCAACCCCCTATACTGTGACTGCCACCTCCGCTGGCTGTCCAGCTGGGTGAAGACTGGCTACAAGGA
ACCGGGCATTGCTCGTTGTGCTGGGCCCCAGGACATGGAGGGCAAGCTGCTCCTCACCACGCCTGCCAAG
AAGTTTGAATGCCAAGGTCCTCCAACGCTGGCTGTCCAGGCCAAGTGTGATCTCTGCTTGTCCAGTCCGT
GCCAGAACCAGGGCACCTGCCACAACGACCCCCTTGAGGTGTACAGGTGCGCCTGCCCCAGCGGCTATAA
GGGTCGAGACTGTGAGGTGTCCCTGGACAGCTGTTCCAGTGGCCCCTGTGAAAATGGGGGCACCTGCCAT
GCACAGGAGGGCGAGGATGCCCCGTTCACGTGCTCCTGTCCCACCGGCTTTGAAGGACCAACCTGTGGGG
TGAACACAGATGACTGTGTGGATCATGCCTGTGCCAATGGGGGCGTCTGTGTGGATGGTGTGGGCAACTA
CACCTGCCAGTGCCCCCTGCAGTATGAGGGAAAGGCCTGTGAGCAGCTGGTGGACTTGTGCTCTCCGGAT
CTGAACCCATGTCAACACGAGGCCCAGTGTGTGGGCACCCCGGATGGGCCCAGGTGTGAGTGCATGCCAG
GTTATGCAGGTGACAACTGCAGTGAGAACCAGGATGACTGCAGGGACCACCGCTGCCAGAATGGGGCCCA
GTGTATGGATGAAGTCAACAGCTACTCCTGCCTCTGTGCTGAGGGCTACAGTGGACAGCTCTGTGAGATC
CCTCCCCATCTGCCTGCCCCCAAGAGCCCCTGTGAGGGGACTGAGTGCCAGAATGGGGCCAACTGTGTGG
ACCAGGGCAACAGGCCTGTGTGCCAGTGCCTCCCAGGCTTCGGTGGCCCTGAGTGTGAGAAGTTGCTCAG
TGTCAACTTTGTGGATCGGGACACTTACCTGCAGTTCACTGACCTGCAAAACTGGCCACGGGCCAACATC
ACGTTGCAGGTCTCCACGGCAGAGGACAATGGGATCCTTCTGTACAACGGGGACAACGACCACATTGCAG
TTGAGCTGTACCAGGGCCATGTGCGTGTCAGCTACGACCCAGGCAGCTACCCCAGCTCTGCCATCTACAG
TGCTGAGACGATCAACGATGGGCAATTCCACACCGTTGAGCTGGTTGCCTTTGACCAGATGGTGAATCTC
TCCATTGATGGCGGGAGCCCCATGACCATGGACAACTTTGGCAAACATTACACGCTCAACAGCGAGGCGC
CACTCTATGTGGGAGGGATGCCCGTGGATGTCAACTCAGCTGCCTTCCGCCTGTGGCAGATCCTCAACGG
CACCGGCTTCCACGGTTGCATCCGAAACCTGTACATCAACAACGAGCTGCAGGACTTCACCAAGACGCAG
ATGAAGCCAGGCGTGGTGCCAGGCTGCGAACCCTGCCGCAAGCTCTACTGCCTGCATGGCATCTGCCAGC
CCAATGCCACCCCAGGGCCCATGTGCCACTGCGAGGCTGGCTGGGTGGGCCTGCACTGTGACCAGCCCGC
TGACGGCCCCTGCCATGGCCACAAGTGTGTCCATGGGCAATGCGTGCCCCTCGACGCTCTTTCCTACAGC
TGCCAGTGCCAGGATGGGTACTCGGGGGCACTGTGCAACCAGGCCGGGGCCCTGGCAGAGCCCTGCAGAG
GCCTGCAGTGCCTGCATGGCCACTGCCAGGCCTCAGGCACCAAGGGGGCACACTGTGTGTGTGACCCCGG
CTTTTCGGGCGAGCTGTGTGAGCAAGAGTCCGAGTGCCGGGGGGACCCTGTCCGGGACTTTCACCAGGTC
CAGAGGGGCTATGCCATCTGCCAGACCACGCGCCCCCTGTCATGGGTGGAGTGCCGGGGCTCGTGCCCAG
GCCAGGGCTGCTGCCAGGGCCTTCGGCTGAAGCGGAGGAAGTTCACCTTTGAGTGCAGCGATGGGACCTC
TTTTGCCGAGGAGGTGGAAAAGCCCACCAAGTGTGGCTGTGCCCTCTGCGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC218562 protein sequence
Red=Cloning site Green=Tags(s)

MALTPGWGSSAGPVRPELWLLLWAAAWRLGASACPALCTCTGTTVDCHGTGLQAIPKNIPRNTERLELNG
NNITRIHKNDFAGLKQLRVLQLMENQIGAVERGAFDDMKELERLRLNRNQLHMLPELLFQNNQALSRLDL
SENAIQAIPRKAFRGATDLKNLQLDKNQISCIEEGAFRALRGLEVLTLNNNNITTIPVSSFNHMPKLRTF
RLHSNHLFCDCHLAWLSQWLRQRPTIGLFTQCSGPASLRGLNVAEVQKSEFSCSGQGEAGRVPTCTLSSG
SCPAMCTCSNGIVDCRGKGLTAIPANLPETMTEIRLELNGIKSIPPGAFSPYRKLRRIDLSNNQIAEIAP
DAFQGLRSLNSLVLYGNKITDLPRGVFGGLYTLQLLLLNANKINCIRPDAFQDLQNLSLLSLYDNKIQSL
AKGTFTSLRAIQTLHLAQNPFICDCNLKWLADFLRTNPIETSGARCASPRRLANKRIGQIKSKKFRCSAK
EQYFIPGTEDYQLNSECNSDVVCPHKCRCEANVVECSSLKLTKIPERIPQSTAELRLNNNEISILEATGM
FKKLTHLKKINLSNNKVSEIEDGAFEGAASVSELHLTANQLESIRSGMFRGLDGLRTLMLRNNRISCIHN
DSFTGLRNVRLLSLYDNQITTVSPGAFDTLQSLSTLNLLANPFNCNCQLAWLGGWLRKRKIVTGNPRCQN
PDFLRQIPLQDVAFPDFRCEEGQEEGGCLPRPQCPQECACLDTVVRCSNKHLRALPKGIPKNVTELYLDG
NQFTLVPGQLSTFKYLQLVDLSNNKISSLSNSSFTNMSQLTTLILSYNALQCIPPLAFQGLRSLRLLSLH
GNDISTLQEGIFADVTSLSHLAIGANPLYCDCHLRWLSSWVKTGYKEPGIARCAGPQDMEGKLLLTTPAK
KFECQGPPTLAVQAKCDLCLSSPCQNQGTCHNDPLEVYRCACPSGYKGRDCEVSLDSCSSGPCENGGTCH
AQEGEDAPFTCSCPTGFEGPTCGVNTDDCVDHACANGGVCVDGVGNYTCQCPLQYEGKACEQLVDLCSPD
LNPCQHEAQCVGTPDGPRCECMPGYAGDNCSENQDDCRDHRCQNGAQCMDEVNSYSCLCAEGYSGQLCEI
PPHLPAPKSPCEGTECQNGANCVDQGNRPVCQCLPGFGGPECEKLLSVNFVDRDTYLQFTDLQNWPRANI
TLQVSTAEDNGILLYNGDNDHIAVELYQGHVRVSYDPGSYPSSAIYSAETINDGQFHTVELVAFDQMVNL
SIDGGSPMTMDNFGKHYTLNSEAPLYVGGMPVDVNSAAFRLWQILNGTGFHGCIRNLYINNELQDFTKTQ
MKPGVVPGCEPCRKLYCLHGICQPNATPGPMCHCEAGWVGLHCDQPADGPCHGHKCVHGQCVPLDALSYS
CQCQDGYSGALCNQAGALAEPCRGLQCLHGHCQASGTKGAHCVCDPGFSGELCEQESECRGDPVRDFHQV
QRGYAICQTTRPLSWVECRGSCPGQGCCQGLRLKRRKFTFECSDGTSFAEEVEKPTKCGCALCA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_003061
ORF Size 4602 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003061.1, NP_003052.1
RefSeq Size 5094 bp
RefSeq ORF 4605 bp
Locus ID 6585
UniProt ID O75093
Cytogenetics 10q24.1
Protein Families Druggable Genome
Protein Pathways Axon guidance
MW 167.7 kDa
Summary Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.UniProtKB/Swiss-Prot Function
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Documents
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"NM_003061" in other vectors (6)
SKU Description Size Price
RC218562L1 Lenti-ORF clone of SLIT1 (Myc-DDK-tagged)-Human slit homolog 1 (Drosophila) (SLIT1) 10 ug
$1,612.00
RC218562L2 Lenti-ORF clone of SLIT1 (mGFP-tagged)-Human slit homolog 1 (Drosophila) (SLIT1) 10 ug
$1,612.00
RC218562L3 Lenti-ORF clone of SLIT1 (Myc-DDK-tagged)-Human slit homolog 1 (Drosophila) (SLIT1) 10 ug
$1,612.00
RC218562L4 Lenti-ORF clone of SLIT1 (mGFP-tagged)-Human slit homolog 1 (Drosophila) (SLIT1) 10 ug
$1,612.00
RG218562 SLIT1 (tGFP-tagged) - Human slit homolog 1 (Drosophila) (SLIT1) 10 ug
$1,512.00
SC303271 SLIT1 (untagged)-Human slit homolog 1 (Drosophila) (SLIT1) 10 ug
$1,444.00

file-alt Citations

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