QRFP (NM_198180) Human Tagged ORF Clone

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SKU
RC218501
QRFP (Myc-DDK-tagged)-Human pyroglutamylated RFamide peptide (QRFP)
  $150.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol QRFP
Synonyms 26RFa; P518
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC218501 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAAGGCCTTACCCCCTGATCTACTTCCTCTTCCTGCCGCTGGGCGCCTGCTTCCCTCTACTGGACA
GAAGAGAGCCCACAGACGCCATGGGTGGCCTCGGAGCTGGAGAACGCTGGGCCGACCTGGCCATGGGGCC
CCGACCCCACTCCGTGTGGGGTTCCTCTCGGTGGCTGAGAGCTTCACAGCCACAGGCCCTGCTTGTCATA
GCCAGGGGGCTGCAGACATCGGGCAGAGAGCATGCTGGCTGCAGATTCCGCTTCGGGAGGCAGGACGAAG
GCAGTGAGGCCACCGGCTTCCTCCCTGCTGCGGGGGAGAAGACCAGCGGCCCGTTAGGGAACCTGGCTGA
GGAGCTCAATGGCTACAGCAGGAAGAAAGGCGGCTTCAGCTTCCGCTTCGGTCGGCGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC218501 protein sequence
Red=Cloning site Green=Tags(s)

MVRPYPLIYFLFLPLGACFPLLDRREPTDAMGGLGAGERWADLAMGPRPHSVWGSSRWLRASQPQALLVI
ARGLQTSGREHAGCRFRFGRQDEGSEATGFLPAAGEKTSGPLGNLAEELNGYSRKKGGFSFRFGRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_198180
ORF Size 408 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_198180.3
RefSeq Size 411 bp
RefSeq ORF 411 bp
Locus ID 347148
UniProt ID P83859
Cytogenetics 9q34.12
MW 14.9 kDa
Summary This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. The encoded products are members of the RFamide family of neuropeptides, characterized by their common protein C-terminus consisting of an arginine (R) and an amidated phenylalanine (F). These products include the neuropeptides 26RFa and the N-terminally extended form, 43RFa. Both of these neuropeptides bind to the pyroglutamylated RFamide peptide receptor (QRFPR) and may regulate blood pressure, reproduction and food intake in rodents. provided by RefSeq, Jul 2015
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Documents
Resources
"NM_198180" in other vectors (4)
SKU Description Size Price
RC218501L3 Lenti ORF clone of Human pyroglutamylated RFamide peptide (QRFP), Myc-DDK-tagged 10 ug
$450.00
RC218501L4 Lenti ORF clone of Human pyroglutamylated RFamide peptide (QRFP), mGFP tagged 10 ug
$450.00
RG218501 QRFP (tGFP-tagged) - Human pyroglutamylated RFamide peptide (QRFP) 10 ug
$350.00
SC307641 QRFP (untagged)-Human pyroglutamylated RFamide peptide (QRFP) 10 ug
$165.00

Plasmid Map for RC218501

Download sequence for

Cloning scheme for RC218501

Circular map for RC218501

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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